Establishing a novel automated magnetic bead-based method for the extraction of DNA from a variety of forensic samples

Witt, Sebastian; Neumann, J.R.; Zierdt, H.; Gébel, Gabriella; Röscheisen, Christiane

doi:10.1016/j.fsigen.2012.01.002

Cited by 54 publications

(19 citation statements)

References 8 publications

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“…63 (B) Pillar-shaped magnets are used for automated transportation, washing, and detection of DNA biomarkers. 51 working principle of the magnetic separator (B); a, metal plate with the reaction tubes containing magnetic particles suspended in buffer solution; b, and c, for the washing steps, the metal plate with the magnets can be moved from one side of the tube to the other; d, carriers for sample lysates; e, carriers for disposable tips; f, waste container; g, magnetic separator; h, carriers for DNA eluates; i, barcode reader, j, liquid handling arm and k, computer. …”

Section: Figure 4 Automated Immunomagnetic Devicesmentioning

confidence: 99%

“…[48][49][50] In addition to such disk magnets, pillar-shaped magnets and microfluidic devices that use micropumps are also being used to transport, wash, and detect desired biomarkers (ie, DNA, C-reactive protein). 51,52 The general direction of such automated devices is towards the assembly of a portable device. Along these lines, Son and Yoon combined quantum dot-coated magnetic particles with a microreactor chip to detect biomarkers of interest.…”

Section: Automationmentioning

confidence: 99%

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Immunomagnetic nanoparticle-based assays for detection of biomarkers

Park¹,

Hwang²,

Lee³

2013

IJN

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The emergence of biomarkers as key players in the paradigm shift towards preventative medicine underscores the need for their detection and quantification. Advances made in the field of nanotechnology have played a crucial role in achieving these needs, and have contributed to recent advances in the field of medicine. Nanoparticle-based immunomagnetic assays, in particular, offer numerous advantages that utilize the unique physical properties of magnetic nanoparticles. In this review, we focus on recent developments and trends with regards to immunomagnetic assays used for detection of biomarkers. The various immunomagnetic assays are categorized into the following: particle-based multiplexing, signal control, microfluidics, microarray, and automation. Herein, we analyze each category and discuss their advantages and disadvantages.

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Section: Figure 4 Automated Immunomagnetic Devicesmentioning

confidence: 99%

Section: Automationmentioning

confidence: 99%

Immunomagnetic nanoparticle-based assays for detection of biomarkers

Park¹,

Hwang²,

Lee³

2013

IJN

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“…Paramagnetic-bead based extraction method of DNA from body fluids such as semen, blood, vaginal, and buccal cells has been established and resulted in a cost-efficient and high-throughput DNA extraction method that can be used for PCR-based molecular Pathology Section testing [21][22][23][24]. The present work explores and evaluates the effectiveness of a superparamagnetic-bead based method (LGC Genomic, Germany) in extracting DNA from DBS.…”

Section: Introductionmentioning

confidence: 99%

“…Manual protocols include the extraction of DNA with Tris-EDTA buffer, methanol, and Chelex-100 [16][17][18][19]. In addition, commercially available kits for DNA extraction from DBS are available [11,20], but their cost could limit their use for large scale-screening programs.Paramagnetic-bead based extraction method of DNA from body fluids such as semen, blood, vaginal, and buccal cells has been established and resulted in a cost-efficient and high-throughput DNA extraction method that can be used for PCR-based molecular Pathology Section testing [21][22][23][24]. The present work explores and evaluates the effectiveness of a superparamagnetic-bead based method (LGC Genomic, Germany) in extracting DNA from DBS.…”

mentioning

confidence: 99%

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR

Sirdah¹

2014

JCDR

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IntrOductIOnDried blood samples spotted on filter papers, introduced by Robert Guthrie in 1963, have been widely used in neonatal screening programs for the early identification of congenital disorders and presymptomatic management of affected neonates [1][2][3]. The DBS were first used in the early 1970s in the neonatal screening for phenylketonuria (PKU) which provided a simple, inexpensive and unique method for heel-stick blood collection from neonates onto a special cotton fiber filter-paper which is still known as a "PKU card" or "Guthrie card" [4,5]. Since then, DBS have been collected routinely from babies not only for PKU screening but also for other biochemical screenings associated with congenital, inherited, and infectious disorders [6][7][8]. The success of these DBS as an easy and cost-effective method for collecting, archiving, and transporting blood specimens has inspired researchers to explore better DNA extraction and PCR-based molecular testing methods for these samples [9][10][11][12]. The DBS sampling is an effective alternative for lymphocyte pellets because DBS sampling requires only a few droplets of whole blood, not necessarily venous blood, and can be directly spotted on Guthrie cards or filter papers [13][14][15].The commonly used methods and techniques for extracting genomic DNA from DBS are varied in terms of extraction principle, reagents used, and quantity and quality of the extracted DNA. Manual protocols include the extraction of DNA with Tris-EDTA buffer, methanol, and Chelex-100 [16][17][18][19]. In addition, commercially available kits for DNA extraction from DBS are available [11,20], but their cost could limit their use for large scale-screening programs.Paramagnetic-bead based extraction method of DNA from body fluids such as semen, blood, vaginal, and buccal cells has been established and resulted in a cost-efficient and high-throughput DNA extraction method that can be used for PCR-based molecular Pathology Section testing [21][22][23][24]. The present work explores and evaluates the effectiveness of a superparamagnetic-bead based method (LGC Genomic, Germany) in extracting DNA from DBS. MAterIAls And MethOdsWhole blood samples collected from 16 apparently healthy subjects and two ß-thalassemia patients were used for spotting onto filter papers, direct DNA extraction and quantitative and qualitative evaluations. spotting onto filter papersFrom each sample four 100 µl applications of whole blood were spotted onto Ahlstrom 226 grad newborn screening filter papers (ID Biological Systems, Greenville, SC, USA), and were dried at ambient temperature (28-30 0 C) for one week. dnA extractionDNA extraction from whole blood and DBS were performed using the superparamagnetic-bead based DNA extraction kit manufactured by LGC Genomic, formerly AGOWA, Germany. The extraction was carried out according to the manufacturer's instruction for whole blood and with some modifications for extraction from DBS. The standard superparamagnetic-bead based DNA extraction protocol includes four princi...

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“…A recent study noted the loss of DNA extracts in a major criminal investigation because the chemicals present in a presumptive test for blood prevented DNA from binding to these beads [97]. this releases the DNA molecules bound to the beads [140]. Since CST method does not employ ethanol, organic solvents, or chaotropic salts there is less chance for carryover of inhibitory elements into the DNA extract.…”

Section: B4 Solid-phase Extractionsmentioning

confidence: 99%

A Novel Method for Rapid and Selective Extraction of Male DNA from Rape Kits using Alkaline Lysis and Pressure Cycling Technology (PCT)

Nori¹

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Establishing a novel automated magnetic bead-based method for the extraction of DNA from a variety of forensic samples

Cited by 54 publications

References 8 publications

Immunomagnetic nanoparticle-based assays for detection of biomarkers

Immunomagnetic nanoparticle-based assays for detection of biomarkers

Superparamagnetic-bead Based Method: An Effective DNA Extraction from Dried Blood Spots (DBS) for Diagnostic PCR

A Novel Method for Rapid and Selective Extraction of Male DNA from Rape Kits using Alkaline Lysis and Pressure Cycling Technology (PCT)

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