Estabelecimento e capacidade infectiva de Gigaspora margarita e Glomus clarum em solo sob erosão

Santos, Anselmo Lúcio dos; Souza, Francisco Adriano de; Berbara, Ricardo Luís Louro; Guerra, José Guilherme Marinho

doi:10.1590/s0102-33062000000200001

Cited by 10 publications

(10 citation statements)

References 20 publications

(11 reference statements)

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“…The viability of AMF propagules in the soil can decrease in accordance with the disturbance to which they are subjected, thus reducing their infective capacity (Trejo;Barois;Sangabriel-Conde, 2016). However, the infective capacity of the AMF species is not related to propagule density (Caproni et al, 2003;Santos et al, 2000), since it infective capacity was not correlated with spore density in the present study. In contrast, dormancy of the spores varies considerably among species (Juge et al, 2002).…”

Section: Inoculum Potentialcontrasting

confidence: 75%

Arbuscular mycorrhizal fungal communities in an iron mining area and its surroundings: Inoculum potential, density, and diversity of spores related to soil properties

et al. 2017

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Arbuscular mycorrhizal fungi (AMF) interact symbiotically with most plant species, facilitating revegetation of areas under rehabilitation. The aim of this study was to evaluate the inoculum potential, density, and diversity of AMF spores in five environments, as well as the relation of species with soil properties. Soil samples were collected in five environments in a mining area and its surroundings in the Quadrilátero Ferrífero, Minas Gerais (Brazil): tailings piles in rehabilitation with grass, canga, Cerrado, native forest, and eucalyptus plantation; these samples were subjected to chemical and physical analyses. Spores were directly extracted from field samples and from trap cultures (TCs) established in two locations in the Southeast and South regions of Brazil for taxonomic identification of the species. Species richness, the Shannon diversity index (H'), and equitability were determined. Principal component analysis (PCA) was used to identify soil properties that most influenced AMF occurrence. Spore density showed no significant difference among the environments. A total of 59 AMF species were found. This is the first report of the occurrence of Acaulospora nivalis and Acaulospora alpina in Brazil. Higher H' and species richness in the field were found in tailings piles and lower in canga. Canga showed higher inoculum potential. The development of TCs in two locations allowed a wider diversity of AMF species to be captured. Environments of the Quadrilátero Ferrífero are hotspots of AMF diversity, and the soil pH and exchangeable S and P contents are the properties that best explain the distribution of AMF species.

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Section: Inoculum Potentialcontrasting

confidence: 75%

Arbuscular mycorrhizal fungal communities in an iron mining area and its surroundings: Inoculum potential, density, and diversity of spores related to soil properties

et al. 2017

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“…Ela apresentou alto NMP de propágulos infectivos e alto número de esporos em todas as áre-as em estudo. Thonson et al (1994) Santos et al (2000). Quando se considera a presença ou ausência das espécies nas áreas em estudo (2, 4, 6, 12 anos de idade e floresta primária), S. erythropa infectou as plantas somente no segundo período de exposição e somente em uma área (Tabela 2), indicando propágulos pouco infectivos ou dormentes.…”

Section: Resultsunclassified

“…A contagem direta de esporos indica a diversidade potencial da população de FMA, ativa e inativa (dormente ou já em senescência). Os dois bioensaios, quando abordados conjuntamente, conforme no presente trabalho, fornecem informações sobre a densidade de propágulos de cada espécie e a agilidade em colonizar (capacidade infectiva) as raízes (Allen, 1991;Santos et al, 2000).…”

Section: Introductionunclassified

Capacidade infectiva de fungos micorrízicos arbusculares em áreas reflorestadas após mineração de bauxita no Pará

Caproni

Franco

Berbara

et al. 2003

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a capacidade infectiva das espécies de fungos micorrízicos arbusculares (FMA) e relacioná-la com o número mais provável (NMP) de propágulos infectivos e número de esporos extraídos diretamente do campo. Amostras de solo foram coletadas em áreas degradadas pela mineração de bauxita com cobertura de 2, 6, 12 e 16 anos após revegetação e em uma área de floresta primária, em Porto Trombetas, PA. Os esporos de FMA foram extraídos e identificados taxonomicamente por suas características morfológicas. A maioria das espécies apresentou comportamento diferente nas áreas em estudo. Glomus macrocarpum foi a que apresentou infectividade mais rápida e alto potencial infectivo, nos solos das cinco áreas estudadas. Esta espécie também apresentou alto NMP de propágulos e alto número de esporos em todas as áreas estudadas. A capacidade infectiva das espécies não está relacionada com a densidade de propágulos. As espécies de FMA possuem diferentes graus de tolerância às condições de solo e se comportaram de maneira diferente de acordo com a idade da revegetação.Termos para indexação: propágulo, esporo, densidade, Amazônia. Infective capacity of arbuscular mycorrhizal fungi in reforested areas after bauxite mining in the Pará State, BrazilAbstract -The objective of this work was to evaluate the infective capacity of arbuscular mycorrhizal fungi (AMF) species and relate it to the most probable number (MPN) of infective propagules and number of spores extracted directly from the field. Soil samples were taken from areas degraded by bauxite mining, 2, 6, 12 and 16 years after reforestation and from an area of primary forest. The spores were extracted and morphologically identified. Most of the species had different behavior for the areas of study. Glomus macrocarpum showed fast infectivity in soils with high infective potential, independently of the soil origin. This species also showed high MPN values of infective propagules and high number of spores in all areas. The infective capacity of the species did not relate to the density of infective propagules. AMF species have different degrees of tolerance to the soil conditions and they behaved in different ways, according to the age of the revegetation.

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“…The genus Gigaspora represents an ecologically and economically (4,42) important group within the Glomeromycota, and numerous studies have been dedicated to identify species within this genus and to study their ecology. The taxonomy of the genus Gigaspora has recently been revised by morphological (5), fatty acid methyl ester (6), molecular (3), and combined (32) approaches.…”

mentioning

confidence: 99%

“…Furthermore, little is known about the establishment, distribution, diversity, and competitiveness of Gigaspora spp. in the field (4,42).…”

mentioning

confidence: 99%

PCR-Denaturing Gradient Gel Electrophoresis Profiling of Inter- and Intraspecies 18S rRNA Gene Sequence Heterogeneity Is an Accurate and Sensitive Method To Assess Species Diversity of Arbuscular Mycorrhizal Fungi of the GenusGigaspora

Souza

Kowalchuk

Leeflang

et al. 2004

Appl Environ Microbiol

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Despite the importance of arbuscular mycorrhizal fungi in the majority of terrestrial ecosystems, their ecology, genetics, and evolution are poorly understood, partly due to difficulties associated with detecting and identifying species. We explored the inter-and intraspecies variations of the 18S rRNA genes of the genus Gigaspora to assess the use of this marker for the discrimination of Gigaspora isolates and of Gigasporaceae populations from environmental samples. Screening of 48 Gigaspora isolates by PCR-denaturing gradient gel electrophoresis (DGGE) revealed that the V3-V4 region of the 18S rRNA gene contained insufficient variation to discriminate between different Gigaspora species. In contrast, the patterns of 18S ribosomal DNA (rDNA) heterogeneity within the V9 region of this marker could be used for reliable identification of all recognized species within this genus. PCR-DGGE patterns provided insight into some putative misidentifications and could be used to differentiate geographic isolates of G. albida, G. gigantea, and G. margarita but not G. rosea. Two major clusters were apparent based upon PCR-DGGE ribotype patterns, one containing G. albida, G. candida, G. ramisporophora, and G. rosea and the other containing G. decipiens and G. margarita. Dissection of the DGGE patterns by cloning, DGGE screening, and sequencing confirmed these groupings and revealed that some ribotypes were shared across species boundaries. Of the 48 isolates examined, only two displayed any spore-to-spore variation, and these exceptions may be indicative of coisolation of more than one species or subspecies within these cultures. Two Brazilian agricultural soils were also analyzed with a Gigasporaceaespecific nested PCR approach, revealing a dominance of G. margarita within this family.

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Estabelecimento e capacidade infectiva de Gigaspora margarita e Glomus clarum em solo sob erosão

Cited by 10 publications

References 20 publications

Arbuscular mycorrhizal fungal communities in an iron mining area and its surroundings: Inoculum potential, density, and diversity of spores related to soil properties

Arbuscular mycorrhizal fungal communities in an iron mining area and its surroundings: Inoculum potential, density, and diversity of spores related to soil properties

Capacidade infectiva de fungos micorrízicos arbusculares em áreas reflorestadas após mineração de bauxita no Pará

PCR-Denaturing Gradient Gel Electrophoresis Profiling of Inter- and Intraspecies 18S rRNA Gene Sequence Heterogeneity Is an Accurate and Sensitive Method To Assess Species Diversity of Arbuscular Mycorrhizal Fungi of the GenusGigaspora

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