Estabelecimento de um patossistema modelo e análise da interação molecular planta-patógeno entre Eucalyptus grandis e Puccinia psidii Winter por meio da técnica de RNA-Seq.

Leite, Thiago Falda

doi:10.11606/t.11.2012.tde-30052012-104104

Cited by 3 publications

(9 citation statements)

References 164 publications

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“…After 72 h.p.i., A. psidii was found only in Eucalyptus grandis and Eucalyptus phaeotricha (susceptible species). Our data is consistent with that of Xavier et al (2001) and Leite (2012). The authors compared the A. psidii infection process in two contrasting genotypes of Eucalyptus grandis and verified that fungal germination, appressoria formation and penetration occur within 12 h.p.i., independent of the genotype.…”

Section: Discussionsupporting

confidence: 90%

The Eucalyptus Cuticular Waxes Contribute in Preformed Defense Against Austropuccinia psidii

et al. 2019

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Austropuccinia psidii, the causal agent of myrtle rust, is a biotrophic pathogen whose growth and development depends on the host tissues. The uredospores of A. psidii infect Eucalyptus by engaging in close contact with the host surface and interacting with the leaf cuticle that provides important chemical and physical signals to trigger the infection process. In this study, the cuticular waxes of Eucalyptus spp. were analyzed to determine their composition or structure and correlation with susceptibility/resistance to A. psidii. Twenty-one Eucalyptus spp. in the field were classified as resistant or susceptible. The resistance/susceptibility level of six Eucalyptus spp. were validated in controlled conditions using qPCR, revealing that the pathogen can germinate on the eucalyptus surface of some species without multiplying in the host. CG-TOF-MS analysis detected 26 compounds in the Eucalyptus spp. cuticle and led to the discovery of the role of hexadecanoic acid in the susceptibility of Eucalyptus grandis and Eucalyptus phaeotricha to A. psidii. We characterized the epicuticular wax morphology of the six previously selected Eucalyptus spp. using scanning electron microscopy and observed different behavior in A. psidii germination during host infection. It was found a correlation of epicuticular morphology on the resistance to A. psidii. However, in this study, we provide the first report of considerable interspecific variation in Eucalyptus spp. on the susceptibility to A. psidii and its correlation with cuticular waxes chemical compounds that seem to play a synergistic role as a preformed defense mechanism.

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Section: Discussionsupporting

confidence: 90%

The Eucalyptus Cuticular Waxes Contribute in Preformed Defense Against Austropuccinia psidii

et al. 2019

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“…De 3 a 9 dias essas manchas se tornam pústulas cheias de urediniósporos que crescem em diâmetro com o tempo, tendo agora uma cor amarelo bastante intensa (XAVIER et al, 2001;LEITE, 2012).…”

Section: Sintomatologiaunclassified

“…A resistência do E. grandis e seus híbridos à P. psidii vem sendo explorada para a obtenção de genótipos superiores desde que a doença se mostrou um problema nas plantações comerciais há mais de 70 anos (JOFFILY, 1944), e alguns poucos trabalhos moleculares objetivaram buscar as bases de resistência dessa espécie contra o patógeno MOON et al, 2007;LEITE, 2012). No trabalho de Leite (2012) criou-se um sistema modelo para o estudo da ferrugem, do qual a presente tese é continuação, e o conhecimento gerado naquele estudo somado à publicação do genoma do E. grandis em 2014 fornecem bases sólidas para as análises de proteômica e metabolômica discutidas neste trabalho.…”

Section: Metabolômica -Modo Negativounclassified

Análise de metabólitos e proteínas totais em folhas de <i>Eucalyptus grandis</i> durante a infecção por <i>Puccinia psidii</i>

Marques¹

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Analysis of total metabolites and proteins in Eucalyptus grandis leaves during the infection by Puccinia psidiiThe molecular mechanisms involved in the plant resistance against pathogens is a well-discussed theme in the academy, overall objecting to diminish worldwide plantation yield losses caused by diseases. Many pathogen-host models were proposed and developed prioritizing model plants and fast growing crops with short life cycles. However, long life cycle species need to cope with the attack of bacteria, fungi and virus throughout many years, or at least until its reproduction period, being unable, meanwhile, to escape the attack of these microorganisms through genetic recombination and mutation. Therefore, as an alternative to the usual models, the present work studied a different pair of antagonists: Eucalyptus grandis and Puccinia psidii. Despite the contribution of plant breeding programs, the E. grandis X P. psidii pathosystem is still poorly described in the molecular level, with few studies about processes and molecules that confer resistance to the plants. Thus, in order to better understand the E. grandis X P. psidii relationship, this project aimed to study the proteome and metabolome changes that occur on leaf tissues of resistant and susceptible plants infected by the pathogen, with the aid of the liquid chromatography coupled to mass spectrometry technique. The results show that the resistant plants notice the presence of the pathogen shortly after being infected, producing immunity related proteins such as HSP90, ILITYHIA, LRR Kinase, NB-ARC disease resistance protein. This perception triggers the production of cell wall and oxidative burst proteins, also changing the primary and secondary metabolism. On the other hand, susceptible plants have its metabolism subverted, producing proteins responsible for the cell wall loosening, favoring P. psidii nutrient uptake, growth and spread. Metabolite biomarkers, Immune response biomarker molecules and infection signals triggered by P. psidii on E. grandis are also proposed on this work.

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“…Observing the germination of spores at 3 hai, apressorium development at 6 hai with penetration starting at 12hai in both resistant and susceptible genotypes. Xavier et al (2001) observed haustorium development in susceptible (18hai) and resistant (24hai), while Leite (2012) observed only in the susceptible genotype at 24hai. Analysing structural aspects, Bini (2016) and Dos Santos et al (2019) characterized wax composition in different genotypes and their involvement in defenses of Eucalyptus spp.…”

Section: Introductionmentioning

confidence: 99%

“…Aiming to characterize the mechanisms involved in disease progression in resistant and susceptible Eucalyptus grandis genotypes infected by A. psidii., Leite (2012) and Xavier et al (2001) analysed initial hours of infection, within 24 hours after inoculation (hai). Observing the germination of spores at 3 hai, apressorium development at 6 hai with penetration starting at 12hai in both resistant and susceptible genotypes.…”

Section: Introductionmentioning

confidence: 99%

Coexpression network analysis of proteins and metabolites time-course of two contrasting >i<Eucalyptus grandis>/i< reponses to >i<Austropuccinia psidii>/i<

Pinheiro¹

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Coexpression network analysis of proteins and metabolites time-course of two contrasting Eucalyptus grandis responses to Austropuccinia psidiiThe development of omics studies as genomics, transcriptomics, proteomics and metabolomics improved greatly our understanding of plant responses to different stresses, including biotic stress, providing insights on mechanisms that determine plant immune system success or pathogen invasion. However, molecular changes orchestrated by trees species in response to fungal infestation and how it is affecting disease outcome involving susceptibility or resistance responses, especially involving primary metabolism reconfigurations, remains poorly understood. Here, a 24h time-course of resistant and susceptible genotypes of Eucalyptus grandis were inoculated and mock-inoculated with Austropuccinia psidii, a biotrophic fungus responsible for rust disease in Eucalyptus, in order to characterize proteins and metabolites changes and divergences among the two selected genotypes. GC-MS based metabolomics and label-free proteomic analysis, were integrated using WGCNA package (Weighted Gene Co-Expression Analysis), to contrast resistant and susceptible responses in Eucalyptus grandis infected with Austropuccinia psidii. Integrated analysis revealed temporal divergences of protein-metabolites modules providing information of differences in photosynthesis and ROS generation, metabolism of lipids, spores germination, metabolism of amino acids, cell wall reinforcement, phytoalexins and defense responses. Our findings provide insights in key molecular mechanisms that underly the interactions between Eucalyptus grandis and Austropuccinia psidii and important features for enhancing molecular breeding efforts.

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Estabelecimento de um patossistema modelo e análise da interação molecular planta-patógeno entre Eucalyptus grandis e Puccinia psidii Winter por meio da técnica de RNA-Seq.

Cited by 3 publications

References 164 publications

The Eucalyptus Cuticular Waxes Contribute in Preformed Defense Against Austropuccinia psidii

The Eucalyptus Cuticular Waxes Contribute in Preformed Defense Against Austropuccinia psidii

Análise de metabólitos e proteínas totais em folhas de <i>Eucalyptus grandis</i> durante a infecção por <i>Puccinia psidii</i>

Coexpression network analysis of proteins and metabolites time-course of two contrasting >i<Eucalyptus grandis>/i< reponses to >i<Austropuccinia psidii>/i<

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