EST analysis of gene expression in the tentacle of <i>Cyanea capillata</i>

Yang, Yan-Bin; Cun, Shujian; Xie, Xiaojin; Lin, Jianghai; Wei, Jianwen; Yang, Wenli; Mou, Chunyan; Yu, Cuiling; Ye, Lanting; Yang, Lu; Fu, Zhiyan; Xu, Anlong

doi:10.1016/s0014-5793(03)00164-9

Cited by 32 publications

(21 citation statements)

References 48 publications

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“…In bilaterians, protease inhibitors restrict their activity according to a tight balance that prevents cellular defects as autodigestion (Witt et al, 2000), abnormal cell migration and metastasis. Related protease inhibitors were identified in cnidarians (Tschesche et al, 1987;Holstein et al, 1992;Yang et al, 2003). Amongst those, the sea anemone elastase inhibitor and the hydra antistatin act as serine protease inhibitors, the former one encoding a Kazal domain (KD), initially characterized in pancreatic tissue as trypsin inhibitor (Rawlings et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Silencing of the hydra serine protease inhibitorKazal1gene mimics the humanSPINK1pancreatic phenotype

Chera

Rosa

Miljkovic‐Licina

et al. 2006

Journal of Cell Science

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In hydra, the endodermal epithelial cells carry out the digestive function together with the gland cells that produce zymogens and express the evolutionarily conserved gene Kazal1. To assess the hydra Kazal1 function, we silenced gene expression through double-stranded RNA feeding. A progressive Kazal1 silencing affected homeostatic conditions as evidenced by the low budding rate and the induced animal death. Concomitantly, a dramatic disorganization followed by a massive death of gland cells was observed, whereas the cytoplasm of digestive cells became highly vacuolated. The presence of mitochondria and late endosomes within those vacuoles assigned them as autophagosomes. The enhanced Kazal1 expression in regenerating tips was strongly diminished in Kazal1(-) hydra, and the amputation stress led to an immediate disorganization of the gland cells, vacuolization of the digestive cells and death after prolonged silencing. This first cellular phenotype resulting from a gene knock-down in cnidarians suggests that the Kazal1 serine-protease-inhibitor activity is required to prevent excessive autophagy in intact hydra and to exert a cytoprotective function to survive the amputation stress. Interestingly, these functions parallel the pancreatic autophagy phenotype observed upon mutation within the Kazal domain of the SPINK1 and SPINK3 genes in human and mice, respectively

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Section: Introductionmentioning

confidence: 99%

Silencing of the hydra serine protease inhibitorKazal1gene mimics the humanSPINK1pancreatic phenotype

Chera

Rosa

Miljkovic‐Licina

et al. 2006

Journal of Cell Science

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“…Nowadays, the EST-based strategy is commonly employed for identifying expressed genes in species of interest [48,49]. This approach has been successfully used to compile a lists of genes expressed in venom's glands of a wide range of animals [50][51][52][53].…”

Section: Transcriptomics Of Lonomia Obliqua Bristle Extractmentioning

confidence: 99%

Toxins from Lonomia obliqua — Recombinant Production and Molecular Approach

Chudzinski-Tavassi¹,

Alvarez-Flores²,

Carrijo-Carvalho³

et al. 2013

An Integrated View of the Molecular Recognition and Toxinology - From Analytical Procedures to Biomedical Applications

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“…Nowadays, the EST-based strategy is commonly used for identifying a large number of genes in species of interest [96,97] . This approach has been suc-cessfully used to generate lists of genes expressed in different venom glands to improve knowledge about toxin diversity in the venom [98][99][100][101] . The identification of genes expressed in cells of a tissue is a basic step to provide essential information about gene function and tissue physiology.…”

Section: Molecular Approachmentioning

confidence: 99%

New Insight into the Mechanism of <i>Lonomia obliqua</i> Envenoming: Toxin Involvement and Molecular Approach

Alvarez-Flores

Zannin²,

Chudzinski-Tavassi³

2010

Pathophysiol Haemos Thromb

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Despite the nearly worldwide distribution of Lepidoptera, there are few species with clear documentation of adverse reactions in humans. Most syndromes caused by Lepidoptera are consequences of direct contact with the hairs or setae of caterpillars. In most instances, the adverse effects caused by moth and caterpillars are self-limited and the treatment is based on the removal of hairs, application of topical antipruritics and, in some cases, the use of oral antihistamines. However, in the case of envenoming by South American Lonomiaobliqua caterpillars, the antilonomic serum produced at Instituto Butantan in Brazil is the only effective treatment to re-establish the physiological coagulation parameters in poisoned patients and to abolish the complications seen in severe cases (e.g. consumptive coagulopathy, intracerebral hemorrhage, and acute renal failure). Many studies have been carried out to understand the pathophysiological mechanism of envenoming by L. obliqua. Several toxic principles were found in bristle extract and the hemolymph, probably related to the envenoming. An interesting fact is that some toxins from the venom usually have more than one function. With the advent of molecular biology techniques it has become possible to analyze these processes at a molecular level, thus giving rise to hypotheses on the molecular basis of envenomation. This review contributes to enhance our understanding of the dramatic alterations that hemorrhagic syndrome causes in patients, current treatment, and the diversity of the molecules involved in this pathology.

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EST analysis of gene expression in the tentacle of Cyanea capillata

Cited by 32 publications

References 48 publications

Silencing of the hydra serine protease inhibitorKazal1gene mimics the humanSPINK1pancreatic phenotype

Silencing of the hydra serine protease inhibitorKazal1gene mimics the humanSPINK1pancreatic phenotype

Toxins from Lonomia obliqua — Recombinant Production and Molecular Approach

New Insight into the Mechanism of <i>Lonomia obliqua</i> Envenoming: Toxin Involvement and Molecular Approach

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