“…Dye reduction tests (Rao and Murthy 1986) seemed to be the first choice, but attempts to apply this principle to selective counts could not be found in the literature. On the other hand, nitrate reduction, also having a long standing use in clinical (Sleigh, 1965, Monteverde andNosanchuk, 198 1, Oneson andGroschel, 198.5, Wilkins et al, 1985) and diary microbiology (Crawley and Twomey, 1969), could be made specific (Mossel, 1964;Crawley and Twomey, 1969). Bomar (1983) suggested the use of an additional glucose dissimilation test to also include in nonspecific counts bacteria failing to reduce nitrate.…”