Essentials of Seed Testing

Justice, Oren L.

doi:10.1016/b978-0-12-395605-7.50011-9

Cited by 16 publications

(10 citation statements)

References 8 publications

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“…Viability was estimated on a sample of 100 seeds using a 0.5% solution of 2,3,5 tri-phenyl tetrazolium salt (Copeland, 1976). Seed water content was estimated according to Justice (1972), using 10 samples of 20 seeds. A sample of 200 seeds was used to estimate their average fresh weight.…”

Section: Plant Materialsmentioning

confidence: 99%

Temperature-dependent germination and endo-beta -mannanase activity in sesame seeds

Carvalho

Borghetti

Buckeridge

et al. 2001

Rev. Bras. Fisiol. Veg.

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The effects of temperature on germination and endo-beta-mannanase activity in seeds of Sesamum indicum was investigated. The minimum germination temperature (Tmin) lies between 12.8°C and 13.2°C while the maximum temperature (Tmax) is located between 45.5°C and 46°C. Germinabilities are statistically not different from estimated viability (88%) between 18.8°C and 43.2°C. The Mann-Whitney test indicated the interval 31.9°C to 35.1°C as the optimum temperature (Topt) range for germination rate. When seeds incubated at temperatures at or below the Tmin and close to or above the Tmax were transferred to 30°C, those incubated at lower temperatures achieved high germinability. On the other hand, the higher the pre-incubation temperature above Tmax, the lower the germinability achieved near Topt. Seed endosperm cell wall was found to contain mannose as the main monosaccharide. An increase in endo-beta-mannanase activity in the micropylar endosperm prior to seed germination was observed only at supra-optimum temperature.

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Section: Plant Materialsmentioning

confidence: 99%

Temperature-dependent germination and endo-beta -mannanase activity in sesame seeds

Carvalho

Borghetti

Buckeridge

et al. 2001

Rev. Bras. Fisiol. Veg.

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“…Their morphological characteristics: colour, shape, texture, areole shape and position, shape of the hilo-micropylar region, spermoderm brightness, seed weight refers the weight of 1,000 seeds according to ISTA (the average of 10 replicates of 100 seeds weight multiply by 10), size, volume, surface, viability and water content were evaluated (Baldwin, 1942;Justice, 1972;ISTA, 1993).…”

Section: Introductionmentioning

confidence: 99%

Effects of Pre-Germination Treatments, Salt and Water Stresses on Germination of Acacia Ehrenbergiana Hayne and Acacia Seyal Del. (Mimosoideae): Two Algerian Native Species

Zetta¹

2017

Appl. Ecol. Env. Res.

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Ben Zetta et al.: Effects of pre-germination treatments, salt and water stresses on germination of two Acacia species - Abstract. Acacia ehrenbergiana Hayne and Acacia seyal Del. are two desert trees of the Algerian Sahara that for a long time were confused. These two species present high potentials for desert areas rehabilitation therefore for successful seed germination and establishment three pre-germination treatments (manual scarification, boiling water and concentrated sulphuric acid) were used to overcome seeds dormancy. Once the optimal conditions are determined, we examined the effects of two abiotic stresses (salinity and water deficiency) on their seeds germination using eight levels of salinity (0,50, 100, 150, 200, 250, 300 and 350mM of NaCl) and six concentrations of PEG 6000 (0, -2, -4, -6, -8 and -10 bars) on Acacia ehrenbergiana Hayne and Acacia seyal Del. Germination rate and mean germination time were calculated and data were subjected to analysis of variance (ANOVA) followed by Newman-Keuls test. Our results show that manual scarification and sulphuric acid had significant effects on the seeds germination of Acacia ehrenbergiana Hayne meanwhile, only sulphuric acid enhanced Acacia seyal Del. seeds germination. Under salt and water stresses, the germination rate and mean germination time of Acacia ehrenbergiana Hayne were less affected than those of Acacia seyal Del. Therefore manual scarification and sulphuric acid treatments could be applied and adopted at laboratory level or even at nurseries to raise plants germination and plantations establishment for desert areas rehabilitation. Thus, Acacia ehrenbergiana Hayne seems to be more suitable for massive propagation as it demonstrates resilience when facing harsh abiotic stresses (salt and water stresses).

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“…In most laboratory tests seeds are classified as germinated when the radicle emerges (Justice 1972). In the experiments reported here germination was deemed to occur when a seedling reached the two-leaf stage, which may be used as an indication of field emergence.…”

Section: Discussionmentioning

confidence: 99%

Seed gerlnination ofSolanum laciniatumAit

Sudiatso

Wilson²

1974

New Zealand Journal of Agricultural Research

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Methods for improving the field germination of Solanum laciniatum were investigated in glasshouse trials. Sct:d was treated at -5 to 35°c for up to 72 hours; soaked in a range of concentrations of potassiuJ? nitra!e, gibberellic acid, indoleacetic acid, and calcium sulphate for up to 9 days; washed III runmng tap water for up to 5 days; and pelleted with a calcium and peat mixture. Soakings in potassium nitrate for 9 days or gibberellic acid for 6 days were the only treatments that significantly increased seedling emergence. There is evidence that poor seedling vigour after germination is a greater problem than poor germination as such.

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Essentials of Seed Testing

Cited by 16 publications

References 8 publications

Temperature-dependent germination and endo-beta -mannanase activity in sesame seeds

Temperature-dependent germination and endo-beta -mannanase activity in sesame seeds

Effects of Pre-Germination Treatments, Salt and Water Stresses on Germination of Acacia Ehrenbergiana Hayne and Acacia Seyal Del. (Mimosoideae): Two Algerian Native Species

Seed gerlnination ofSolanum laciniatumAit

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