Essentiality of Boron for Symbiotic Dinitrogen Fixation in Pea (Pisum sativum) Rhizobium Nodules

Abstract

The effect of boron deficiency on symbiotic nitrogen fixation in pea (Pisum safivum) was examined. l h e absence of boron in the culture medium resulted in a decrease of the number of nodules and an alteration of nodule development leading to an inhibition of nitrogenase activity. Examination of boron-deficient nodules showed dramatic changes in cell walls and in both peribacteroid and infection thread membranes, suggesting a role for boron in the stability of these structures. These results indicate that boron is a requirement for normal nodule development and functionality.

Role of Boron Nutrient in Nodules Growth and Nitrogen Fixation in Soybean Genotypes Under Water Stress Conditions

“…The current results showed that, even though B concentration in leaves was above the critical level (20 mg B kg -1 , critical level of B in leaves for normal plant growth) [44], FB resulted in a positive effect on seed and nodule weights, agreeing with previous research of those of [7,43,45]. Our results showed that foliar B increased grain weight, nodule mass and number due to the stimulatory effects of B on growth and development [9,11] and nodule improvement.…”

“…A lower level of infection of the host plants with Rhizobium was noticed in plants grown in B-deficient medium compared to plants supplied with adequate B [9]. It was shown that that little or no ability to fix N 2 under Bdeficient plants [11]. Recently, it was found that FB increased nodule weight under irrigated greenhouse conditions [43].…”

“…Boron is required for nodules growth and nitrogen fixation [9,[11][12][13], and boron deficiency can occur under certain environmental stress factors even when boron level in soil is adequate [19], leading to yield loss. Among the environmental stress factors that can lead to boron deficiency in plants is drought or water stress.…”

“…Previous research indicated the involvement of B in cell wall structure [5,6]; cell membrane integrity [2,7]; sugar metabolism [2], especially sugar alcohols [3,8]; nitrogen assimilation and fixation [9,10]; nodules [11,12], nodullin protein (ENOD2) and malfunction of oxygen diffusion barrier [13]; phenolic metabolism [2,14,15]; ion uptake [2,16]; and plasma membrane-bount H+ ATPase [7,17,18].…”

Mogelijke oorzaken van een dalende opbrengst van witte klaver (Trifolium repens L.) in de tijd

“…Leguminosen, waaronder witte klaver, hebben daarnaast relatief veel B nodig voor de vorming van wortelknolletjes; het B-gehalte in de knolletjes is 4-5 keer zo hoog als in de wortels (Carpena et al 2000). Een B-tekort leidt tot verminderde vorming en verstoorde ontwikkeling van de wortelknolletjes (Bolanos et al 1994). Borium is nodig voor de vorming van infectiedraden en het binnendringen van Rhizobium-bacteriën in de wortelcellen.…”