“…JAK2 mutation was detected by allele-specific polymerase chain reaction (PCR), using mutant-specific primer (MF: 5 0 -AGC ATT TGG TTT TAA ATT ATG GAG TAT ATT-3 0 ), an internal control primer (ICF: 5 0 -ATC TAT AGT CAT GCT GAA AGT AGG AGA AAG-3 0 ), and a reverse primer (R: 5 0 -CTG AAT AGT CCT ACA GTG TTT TCA GTT TCA-3 0 ) [2]. The PCR mixture contained 0.2 mM dNTP, 1 mmol/L primer R, 0.5 mmol/L of primers MF and ICF, 50 ng DNA template, 2.0 mM MgCl 2 , 1Â PCR buffer, and 1 U AmpliTaq DNA polymerase (Applied Biosystem, Foster City, CA, USA) in a total volume of 25 mL.…”