Essential Roles of the Tap42-Regulated Protein Phosphatase 2A (PP2A) Family in Wing Imaginal Disc Development of Drosophila melanogaster

Wang, Ning; Leung, Hung-Tat; Mazalouskas, Matthew D.; Watkins, Guy R.; Gomez, Rey J.; Wadzinski, Brian E.

doi:10.1371/journal.pone.0038569

Cited by 9 publications

(5 citation statements)

References 49 publications

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“…Overexpression of the inhibitor of matrix metalloproteases ( Timp ) results in pupal lethality and cleft thorax (Srivastava et al 2007). Finally, wing disc-specific knockdown of Tap42, a key regulator of protein phosphatases, gives rise to cleft thorax and to wing abnormalities similar to some that we observed ( Wang et al 2012 ).…”

Section: Discussionsupporting

confidence: 82%

Ligand-Bound GeneSwitch Causes Developmental Aberrations inDrosophilathat Are Alleviated by the Alternative Oxidase

Andjelković

Kemppainen

Jacobs

2016

G3 Genes|Genomes|Genetics

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Culture of Drosophila expressing the steroid-dependent GeneSwitch transcriptional activator under the control of the ubiquitous α-tubulin promoter was found to produce extensive pupal lethality, as well as a range of dysmorphic adult phenotypes, in the presence of high concentrations of the inducing drug RU486. Prominent among these was cleft thorax, seen previously in flies bearing mutant alleles of the nuclear receptor Ultraspiracle and many other mutants, as well as notched wings, leg malformations, and bristle abnormalities. Neither the α-tubulin-GeneSwitch driver nor the inducing drug on their own produced any of these effects. A second GeneSwitch driver, under the control of the daughterless promoter, which gave much lower and more tissue-restricted transgene expression, exhibited only mild bristle abnormalities in the presence of high levels of RU486. Coexpression of the alternative oxidase (AOX) from Ciona intestinalis produced a substantial shift in the developmental outcome toward a wild-type phenotype, which was dependent on the AOX expression level. Neither an enzymatically inactivated variant of AOX, nor GFP, or the alternative NADH dehydrogenase Ndi1 from yeast gave any such rescue. Users of the GeneSwitch system should be aware of the potential confounding effects of its application in developmental studies.

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Section: Discussionsupporting

confidence: 82%

Ligand-Bound GeneSwitch Causes Developmental Aberrations inDrosophilathat Are Alleviated by the Alternative Oxidase

Andjelković

Kemppainen

Jacobs

2016

G3 Genes|Genomes|Genetics

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“…Mutants of lowfat have shorter, rounder wings in Drosophila (Hogan et al, 2011; Mao et al, 2009), whereas dok mutants have shrivelled wings (Biswas et al, 2006). The knirps‐related protein ( knrl ) is involved in second wing vein development (Table 3, Lunde et al, 2003), and Tap42 (Figure 4b) triggers apoptosis in the developing wing discs (Wang et al, 2012). Thus, we have identified some promising genes that could be studied further in this system in future.…”

Section: Discussionmentioning

confidence: 99%

Genomics of altitude‐associated wing shape in two tropical butterflies

et al. 2021

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Climate change is forcing organisms to "move, adapt, or die". With temperatures rising and land-use changing in the lowlands, shifting to higher elevations might be the only way to flee extinction for many taxa (Chen et al., 2011). However, the environment changes drastically along mountains, with diverse sets of challenges expected to drive local adaptation (Halbritter et al., 2015). Thus, identifying the genomic mechanisms that allow organisms to inhabit wide ranges is key to understanding which taxa are most likely to adapt locally when forced to colonise new, high-elevation, environments. With novel sequencing technologies and the explosion of genomic

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“…The knirps-related protein (knrl) is involved in second wing vein development (Table 3, Lunde et al, 2003), and Tap42 (Fig. 4B) triggers apoptosis in the developing wing discs (Wang et al, 2012). Interestingly, a recent study found that knockdowns of fat and knirps affected wing shape in D. melanogaster, which regulate two of our candidate genes (Table 3, Pitchers et al, 2019).…”

Section: Candidates Genes Associated With Wing Shape Variation In Othmentioning

confidence: 91%

Genomics of altitude-associated wing shape in two tropical butterflies

Montejo‐Kovacevich

Salazar

Smith

et al. 2020

Preprint

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Understanding how organisms adapt to their local environment is central to evolution. With new whole-genome sequencing technologies and the explosion of data, deciphering the genomic basis of complex traits that are ecologically relevant is becoming increasingly feasible. Here we study the genomic basis of wing shape in two Neotropical butterflies that inhabit large geographical ranges. Heliconius butterflies at high elevations have been shown to generally have rounder wings than those in the lowlands. We reared over 1100 butterflies from 71 broods of H. erato and H. melpomene in common-garden conditions and show that wing aspect ratio, i.e. elongatedness, is highly heritable in both species and elevation-associated wing shape differences are maintained. Genome-wide associations with a published dataset of 666 whole genomes from across a hybrid zone, uncovered a highly polygenic basis to wing shape variation in the wild. We identify several genes that have roles in wing morphogenesis or wing shape variation in Drosophila flies, making them promising candidates for future studies. There was little evidence for molecular parallelism in the two species, with only one shared candidate gene, nor for a role of the four known colour pattern loci, except for optix in H. erato. Thus, we present the first insights into the heritability and genomic basis of within-species wing shape in two Heliconius species, adding to a growing body of evidence that polygenic adaptation may underlie many ecologically relevant traits.

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Essential Roles of the Tap42-Regulated Protein Phosphatase 2A (PP2A) Family in Wing Imaginal Disc Development of Drosophila melanogaster

Cited by 9 publications

References 49 publications

Ligand-Bound GeneSwitch Causes Developmental Aberrations inDrosophilathat Are Alleviated by the Alternative Oxidase

Ligand-Bound GeneSwitch Causes Developmental Aberrations inDrosophilathat Are Alleviated by the Alternative Oxidase

Genomics of altitude‐associated wing shape in two tropical butterflies

Genomics of altitude-associated wing shape in two tropical butterflies

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