Essential roles of stat5.1 / stat5b in controlling fish somatic growth

Xiong, Shuting; Mei, Jie; Huang, Peipei; Jing, Jing; Li, Zhi; Kang, Jingliang; Gui, Jian‐Fang

doi:10.1016/j.jgg.2017.07.006

Cited by 29 publications

(24 citation statements)

References 43 publications

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“…Primer sequences are listed in Supplementary Table 1. Probe hybridization was performed as described previously 36 . Whole-mount embryos were imaged using Leica DFC550 with LAS 4.6 software.…”

Section: Methodsmentioning

confidence: 99%

Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish

et al. 2020

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Early embryogenesis relies on maternally inherited mRNAs. Although the mechanism of maternal mRNA degradation during maternal-to-zygotic transition (MZT) has been extensively studied in vertebrates, how the embryos maintain maternal mRNA stability remains unclear. Here, we identify Igf2bp3 as an important regulator of maternal mRNA stability in zebrafish. Depletion of maternal igf2bp3 destabilizes maternal mRNAs prior to MZT and leads to severe developmental defects, including abnormal cytoskeleton organization and cell division. However, the process of oogenesis and the expression levels of maternal mRNAs in unfertilized eggs are normal in maternal igf2bp3 mutants. Gene ontology analysis revealed that these functions are largely mediated by Igf2bp3-bound mRNAs. Indeed, Igf2bp3 depletion destabilizes while its overexpression enhances its targeting maternal mRNAs. Interestingly, igf2bp3 overexpression in wild-type embryos also causes a developmental delay. Altogether, these findings highlight an important function of Igf2bp3 in controlling early zebrafish embryogenesis by binding and regulating the stability of maternal mRNAs.

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Section: Methodsmentioning

confidence: 99%

Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish

et al. 2020

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“…In mouse, Stat5b is a critical regulator of male-biased size dimorphism ( Udy et al, 1997 ). Previous studies indicated that stat5b (also known as stat5.1 ) control somatic growth and is involved in sexual size dimorphism in adult zebrafish ( Xiong et al, 2017 ). To explore the molecular mechanism underlying sexual size dimorphism, we performed a comparative transcriptome analysis on wildtype and stat5b -mutated (both sexes) to characterize the function of stat5b and its downstream genes and pathways.…”

Section: Discussionmentioning

confidence: 99%

“…All experiments involved zebrafish were approved by the institution animal care and use committee of Huazhong Agricultural University. Stat5b -deficient zebrafish was generated by CRISPR/CAS9 technology by our previous method ( Xiong et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we found that stat5b (also known as stat5.1 ) regulates body growth in zebrafish. Moreover, female-biased size dimorphism was abolished in stat5b -mutated adult homozygous zebrafish ( Xiong et al, 2017 ). In the present study, comparative transcriptome analysis was performed to assess the effect of stat5b deficiency on the sex-specific gene expression in zebrafish liver.…”

Section: Introductionmentioning

confidence: 99%

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Stat5b Regulates Sexually Dimorphic Gene Expression in Zebrafish Liver

et al. 2018

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Sexual size dimorphism is an interesting phenomenon occurred in many fish species. Wildtype zebrafish exhibits a significant sexual dimorphism in body size at the adult stage. Previous studies indicated that sexual size dimorphism was eliminated in stat5b-mutated zebrafish. Herein, the comparative transcriptome analysis was conducted to observe the genes and pathways involved in sexual size dimorphism. The number of male-biased and female-biased genes was much less in the liver of stat5b mutant zebrafish than in wildtype. Gene ontology (GO) enrichment and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that multiple pathways related to metabolism were affected upon loss of stat5b function. qRT-PCR results also validated that sexually dimorphic expression of a set of genes was lost when stat5b was mutated. Furthermore, the weighted correlation network analysis (WGCNA) detected many candidate genes related to the growth traits and stat5b function, such as greb1, lepr, and igf2b. Our data suggest that stat5b should regulate the sexually dimorphic gene expression in zebrafish liver and add in understanding of the molecular mechanisms underlying sexual size dimorphism in fish species.

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“…Gene mutagenesis mediated by CRISPR/Cas9 was performed as previously described [24,25]. The guide RNA (gRNA) target sites were designed by an online tool, ZIFIT Targeter (http://zifit.partners.org/ZiFiT/CSquare9Nuclease.aspx).…”

Section: Transgenic Overexpression and Targeted Gene Disruption Of Pfmentioning

confidence: 99%

A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)

et al. 2018

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Essential roles of stat5.1 / stat5b in controlling fish somatic growth

Cited by 29 publications

References 43 publications

Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish

Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish

Stat5b Regulates Sexually Dimorphic Gene Expression in Zebrafish Liver

A novel PDZ domain-containing gene is essential for male sex differentiation and maintenance in yellow catfish (Pelteobagrus fulvidraco)

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