Essential Roles of Monocytes in Stimulating Human Peripheral Blood Mononuclear Cells with
            <i>Lactobacillus casei</i>
            To Produce Cytokines and Augment Natural Killer Cell Activity

Shida, Katsunori; Suzuki, Tomomi; Kiyoshima-Shibata, Junko; Shimada, Satoru; Nanno, Masanobu

doi:10.1128/cvi.00076-06

Cited by 77 publications

(80 citation statements)

References 40 publications

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“…Our data is consistent with previous studies showing that probiotic bacteria are able to induce TNF-α secretion in human PBMC [8,10,11,17] . However, there were considerable differences in the ability of different probiotic bacteria to induce IL-12 and IFN-γ.…”

Section: Discussionsupporting

confidence: 83%

“…In human PBMCs gram-positive bacteria induce TNF-α [8][9][10] , IL-12 [11][12][13][14][15] and IFN-γ [10,11,13,15] while gramnegative bacteria have preferentially been suggested to induce IL-10 production [14] . Gram-positive bacteria or their structural components activate cells via TLR2, whereas gram-negative bacteria and their major structural component lipopolysaccharide activate host cell via TLR4 [32] .…”

Section: Discussionmentioning

confidence: 99%

“…IL-10 downregulates the inflammatory response and induces an antibodymediated immune response [7] . In previous studies it has been shown that individual probiotic bacteria can induce the production of TNF-α [8][9][10] , IL-12 [11][12][13][14][15] , IFN-γ [10,11,13,15] and IL-10 [9,[15][16][17][18] in human peripheral blood mononuclear cells (PBMC) in vitro. At present only a limited amount of comparative data is available on the ability of different probiotic strains to induce cytokine production within the same experimental system [9,[14][15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

Kekkonen¹,

Kajasto²,

Miettinen³

et al. 2008

WJG

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show abstract

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

Kekkonen¹,

Kajasto²,

Miettinen³

et al. 2008

WJG

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show abstract

“…Thus, live Lactobacillus casei Shirota (LcS) was able to enhance NK activity in PBMCs [31]. Besides, heat-killed LcS stimulated IL-10, IL-12 and TNF-α and promoted NK cells activity [32]. In our model, combinations of LcV+PppA, LcM+PppA, LcV and LcM increased IL-2 induction; thus, this fact, together with IL-10 increase, would be involved in NK stimulation.…”

Section: Discussionmentioning

confidence: 74%

Specific Immune Response Induced by a Lactobacillus Associated with a Pneumococcal Antigen in an “in vitro” Human Cells Model

Vintiñi¹

2012

J Vaccines Vaccin

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In this work we assessed the immune response induced by combinations of live (LcV) and heat-killed (LcM) Lactobacillus casei CRL 431 as adjuvants associated with pneumococcal protective A protein (PppA) in peripheral blood mononuclear cells (PBMCs). LcV, LcM and their combinations with PppA stimulated T, B and NK cells. Thus, all stimuli increased CD25 expression in T CD3 lymphocytes, highest activation being reached with the combinations of LcV or LcM with an antigen (PppA+LcV, PppA+LcM). Expression of CD19 B cells marker was significantly increased in almost all treatments compared with non-stimulated PBMCs, except for PppA. All treatments increased CD86 expression in the LT population, while in B cells only LPS, PppA+LcV and PppA+LcM increased it. NK cells were significantly increased by LPS (P<0.05), PppA+LcM (P<0.01) and PppA+LcV (P<0.01) compared to non-stimulated PBMCs. PppA+LcV and PppA+LcM increased CD56 expression in both NKT and NK cells, while LcM expanded NKT population. Cytokine pattern analysis showed that LcV and LcM stimulated Th, Th2 and Th17 cytokines and exerted an important adjuvant effect when associated with PppA. Correlation with previous results obtained in animal models when the same experimental vaccine was nasally administered is discussed. Human PBMCs would be useful to evaluate the immune response of mucosal vaccines containing lactic acid bacteria associated with a specific antigen.

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“…Nevertheless, there is growing evidence that probiotics have immunomodulatory properties and that these properties of probiotics are strain-dependent (32)(33)(34)(35)(36)(37)(38)(39)(40)(41) . For example, there is consistent evidence that a number of probiotics enhance natural killer (NK) cell activity in vitro (40)(41)(42)(43) and there is some supportive evidence that this also occurs in vivo (44)(45)(46) . Probiotic strains whose cell walls are resistant to digestion appear to be particularly potent enhancers of NK activity; it is suggested that monocytes phagocytose the probiotic bacteria and the insoluble cell wall components induce the production of IL-12, which augments NK cell activity (38,47) .…”

Section: Probiotics Ageing and Immunitymentioning

confidence: 99%

Ageing, immunity and influenza: a role for probiotics?

Yaqoob

2013

Proc. Nutr. Soc.

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Influenza is a major cause of death in the over 65s. Increased susceptibility to infection and reduced response to vaccination are due to immunosenscence in combination with medical history and lifestyle factors. Age-related alterations in the composition of the gut microbiota have a direct impact on the immune system and it is proposed that modulation of the gut microbiota using pre- and probiotics could offer an opportunity to improve immune responses to infections and vaccination in older people. There is growing evidence that probiotics have immunomodulatory properties, which to some extent are strain-dependent, and are strongly influenced by ageing. Randomised controlled trials suggest that probiotics may reduce the incidence and/or severity of respiratory infections, although there is limited data on older people. A small number of studies have examined the potential adjuvant effects of selected probiotics for vaccination against influenza; however, the data is inconsistent, particularly in older people. This review describes the impact of age-related changes in the gut on the immune response to respiratory infections and evaluates whether restoration of gut microbial homoeostasis by probiotics offers an opportunity to modulate the outcome of respiratory infections and vaccination against influenza in older people. Although there is promising evidence for effects of probiotics on human health, there is a lack of consistent data, perhaps partly due to strain-specific differences and an influence of the age of the host. Further research is critical in evaluating the potential use of probiotics in respiratory infections and vaccination in the ageing population.

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Essential Roles of Monocytes in Stimulating Human Peripheral Blood Mononuclear Cells with Lactobacillus casei To Produce Cytokines and Augment Natural Killer Cell Activity

Abstract: We examined the effect of a probiotic strain, Lactobacillus casei strain Shirota, on cytokine production and natural killer (NK) cell activity in human peripheral blood mononuclear cells (

Cited by 77 publications

References 40 publications

Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

Probiotic Leuconostoc mesenteroides ssp. cremoris and Streptococcus thermophilus induce IL-12 and IFN-γ production

Specific Immune Response Induced by a Lactobacillus Associated with a Pneumococcal Antigen in an “in vitro” Human Cells Model

Ageing, immunity and influenza: a role for probiotics?

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