Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish

Lee, Gang Hui; Chang, Min Yen; Hsu, Cheng‐Hsing; Chen, Yau Hung

doi:10.1007/s00018-011-0637-2

Cited by 25 publications

(25 citation statements)

References 38 publications

(57 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the next day, tcf21 expression refines to the cores of the pharyngeal arches, where it is surrounded by neural crest cells, and splits into dorsal and ventral clusters, which then divide further into smaller clusters (Figures 1G–1I). Later, mCherry from the tcf21:mCherry transgene labels most of the head muscles (Figures 1L, 1M, and 4A–4D) as suggested previously [20–22, 26, 27]. However, mCherry also labels some of the dorsal and most of the ventral head vasculature, including part of the lateral dorsal aorta (LDA), the hypobranchial arteries (HAs), the pPAAs, and parts of the ventral aorta (VA) (Figures 1N, 1O, 4Q–4T, and 5A–5D).…”

Section: Resultssupporting

confidence: 82%

“…Our and others’ genetic analyses show that the tcf21 + pharyngeal head muscle progenitors require Tcf21 activity for their specification [22, 26]. Similarly, we find that the tcf21 +/ nkx2.5 + vascular HA and pPAA progenitors require the joint activity of Tcf21 and Nkx2.5 for their specification, and previous studies have shown that Nkx2.5 is required for ventricular identity in the heart [43–46].…”

Section: Discussionsupporting

confidence: 85%

See 1 more Smart Citation

Origin, Specification, and Plasticity of the Great Vessels of the Heart

Nagelberg

Wang

et al. 2015

Current Biology

View full text Add to dashboard Cite

SUMMARY The pharyngeal arch arteries (PAAs) are a series of paired embryonic blood vessels that give rise to several major arteries that connect directly to the heart. During development, the PAAs emerge from nkx2.5-expressing mesodermal cells and connect the dorsal head vasculature to the outflow tract of the heart. Despite their central role in establishing the circulatory system, the embryonic origins of the PAA progenitors are only coarsely defined, and the factors that specify them and their regenerative potential are unclear. Using fate mapping and mutant analysis, we find that PAA progenitors are derived from the tcf21 and nkx2.5 double-positive head mesoderm and require these two transcription factors for their specification and survival. Unexpectedly, cell ablation shows that the tcf21+; nkx2.5+ PAA progenitors are not required for PAA formation. We find that this compensation is due to the replacement of ablated tcf21+; nkx2.5+ PAA cells by endothelial cells from the dorsal head vasculature. Together, these studies assign the embryonic origin of the great vessel progenitors to the interface between the pharyngeal and cardiac mesoderm, identify the transcription factor code required for their specification, and reveal an unexpected plasticity in the formation of the great vessels.

show abstract

Section: Resultssupporting

confidence: 82%

Section: Discussionsupporting

confidence: 85%

Origin, Specification, and Plasticity of the Great Vessels of the Heart

Nagelberg

Wang

et al. 2015

Current Biology

View full text Add to dashboard Cite

show abstract

“…embryos were washed with PBS and fixed in 4% paraformaldehyde. The procedures for whole-mount in situ hybridization, vascular alkaline phosphatase staining, and cryosections have been described previously (Pai and Chen 2010;Lee et al 2011), except that flk/kdrl (Lawson et al 2002) were used as probe.…”

Section: Methodsmentioning

confidence: 99%

von Hippel–Lindau gene plays a role during zebrafish pronephros development

Chen

Chang

Lai

et al. 2015

In Vitro Cell.Dev.Biol.-Animal

Self Cite

View full text Add to dashboard Cite

von Hippel-Lindau (pVHL)-mediated ubiquitination of HIF-1α plays a central role in the cellular responses to changes in oxygen availability. In the present study, using zebrafish as a model, we showed that specific knockdown of endogenous vhl leads to pronephros malformation and renal failure. Knockdown of vhl resulted in abnormal kidney development, including curved and cystic pronephric tubule or/and cystic and atrophic glomerulus. Co-injecting capped vhl messenger RNA (mRNA) partially rescued pronephros morphant phenotype, confirming the specificity of the morpholino oligonucleotide (MO)-induced pronephric defects. In keeping with the pronephros phenotype, renal function was affected as well in vhl morphants. Dextran clearance abilities of vhl morphants were significantly reduced as compared with those of control embryos. Further analysis indicated that glomerular integrity is impaired in vhl morphants, while the organization of pronephric duct was minimally affected. Vhl morphants display global increased vegf signaling and angiogenesis. In addition, we found that vhl morphants displayed elevated expression of vegfa in podocytes and increased angiogenesis at pronephric glomerulus and the nearby vessels. Treatment of vegf inducer to embryos also caused pronephros phenotype resembling vhl morphants, further supporting that increased vegfa signaling contribute to the pronephros morphant phenotype. Our study establishes the zebrafish as an alternative vertebrate model system for studying Vhl function during kidney development.

show abstract

“…4J). As a mesodermal marker, we monitored the expression of tcf21, which is also known as capsulin in zebrafish (Lee et al, 2011). In pax1 mutants, the expression of tcf21 marked the mesodermal cores of the first and second arches, but the posterior expression never exhibited the reiterative distribution of the arch cores (Fig.…”

Section: Reiterative Development Of the Third And Posterior Pouches Rmentioning

confidence: 99%

Reiterative expression of pax1 directs pharyngeal pouch segmentation in medaka (Oryzias latipes)

et al. 2016

View full text Add to dashboard Cite

A striking characteristic of vertebrate development is the pharyngeal arches, which are a series of bulges on the lateral surface of the head of vertebrate embryos. Although each pharyngeal arch is segmented by the reiterative formation of endodermal outpocketings called pharyngeal pouches, the molecular network underlying the reiterative pattern remains unclear. Here, we show that pax1 plays crucial roles in pouch segmentation in medaka (Oryzias latipes) embryos. Importantly, pax1 expression in the endoderm prefigures the location of the next pouch before the cells bud from the epithelium. TALEN-generated pax1 mutants did not form pharyngeal pouches posterior to the second arch. Segmental expression of tbx1 and fgf3, which play essential roles in pouch development, was almost nonexistent in the pharyngeal endoderm of pax1 mutants, with disturbance of the reiterative pattern of pax1 expression. These results suggest that pax1 plays a key role in generating the primary pattern for segmentation in the pharyngeal endoderm by regulating tbx1 and fgf3 expression. Our findings illustrate the crucial roles of pax1 in vertebrate pharyngeal segmentation and provide insights into the evolutionary origin of the deuterostome gill slit.

show abstract

Essential roles of basic helix-loop-helix transcription factors, Capsulin and Musculin, during craniofacial myogenesis of zebrafish

Cited by 25 publications

References 38 publications

Origin, Specification, and Plasticity of the Great Vessels of the Heart

Origin, Specification, and Plasticity of the Great Vessels of the Heart

von Hippel–Lindau gene plays a role during zebrafish pronephros development

Reiterative expression of pax1 directs pharyngeal pouch segmentation in medaka (Oryzias latipes)

Contact Info

Product

Resources

About