Essential role of Msx1 in regulating anterior-posterior patterning of the secondary palate in mice

Zhu, Shicheng; Song, Hanjing; Liu, Zhong; Huo, Suman; Fang, Yukun; Zhao, Wanxin; Yang, Xueqin; Dai, Zhongmin; He, Rui; Qiu, Mengsheng; Zhang, Zunyi; Zhu, Xiaojing

doi:10.1016/j.jgg.2021.07.006

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…Consistent with previous studies, our study demonstrates significant heterogeneity among mesenchymal cells throughout palatal development. The marker genes used to identify the 5 mesenchymal clusters not only exhibited high expression levels in each subcluster in the present study but have also been recognized for their important roles during palate development in previous studies (Jin and Ding 2006;Cesario et al 2015;Xu et al 2019;Han et al 2021;Zhu et al 2022). Our study identified the cell populations to which these regulatory genes belong and investigated their spatial distribution and functional properties.…”

Section: Discussionmentioning

confidence: 66%

Spatiotemporal Evolution of Developing Palate in Mice

Wang,

Zhang,

Zhao

et al. 2024

J Dent Res

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The intricate formation of the palate involves a series of complex events, yet its mechanistic basis remains uncertain. To explore major cell populations in the palate and their roles during development, we constructed a spatiotemporal transcription landscape of palatal cells. Palate samples from C57BL/6 J mice at embryonic days 12.5 (E12.5), 14.5 (E14.5), and 16.5 (E16.5) underwent single-cell RNA sequencing (scRNA-seq) to identify distinct cell subsets. In addition, spatial enhanced resolution omics-sequencing (stereo-seq) was used to characterize the spatial distribution of these subsets. Integrating scRNA-seq and stereo-seq with CellTrek annotated mesenchymal and epithelial cellular components of the palate during development. Furthermore, cellular communication networks between these cell subpopulations were analyzed to discover intercellular signaling during palate development. From the analysis of the middle palate, both mesenchymal and epithelial populations were spatially segregated into 3 domains. The middle palate mesenchymal subpopulations were associated with tooth formation, ossification, and tissue remodeling, with initial state cell populations located proximal to the dental lamina. The nasal epithelium of the palatal shelf exhibited richer humoral immune responses than the oral side. Specific enrichment of Tgfβ3 and Pthlh signals in the midline epithelial seam at E14.5 suggested a role in epithelial–mesenchymal transition. In summary, this study provides high-resolution transcriptomic information, contributing to a deeper mechanistic understanding of palate biology and pathophysiology.

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Section: Discussionmentioning

confidence: 66%

Spatiotemporal Evolution of Developing Palate in Mice

Wang,

Zhang,

Zhao

et al. 2024

J Dent Res

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Epidemiological and clinical evaluation of patients with a cleft in lower saxony Germany: a mono-center analysis

Philipp,

Anja,

Boris

et al. 2023

Clin Oral Invest

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Objective The aim was to provide epidemiological and clinical data on patients with orofacial clefts in Lower Saxony in Germany. Materials and methods The records of 404 patients with orofacial clefts treated surgically at the University Medical Center Goettingen from 2001 to 2019 were analyzed in this retrospective study. Prevalence of orofacial clefts in general, orofacial clefts as manifestation of a syndrome, sex distribution, and prevalence of different cleft types was evaluated and associated with the need for corrective surgery, family history, pregnancy complications, and comorbidities. Results The prevalence of orofacial clefts for Goettingen in Lower Saxony was 1:890. 231 patients were male and 173 were female. CLP was most common (39.1%) followed by CP (34.7%), CL (14.4%), CLA (9.9%), and facial clefts (2%). The left side was more frequently affected and unilateral cleft forms occurred more often than bilateral ones. Almost 10% of the population displayed syndromic CL/P. 10.9% of all patients had a positive family history regarding CL/P, predominantly from the maternal side. Pregnancy abnormalities were found in 11.4%, most often in the form of preterm birth. Comorbidities, especially of the cardiovascular system, were found in 30.2% of the sample. 2.2% of patients treated according to the University Medical Center Goettingen protocol corrective surgery was performed in form of a velopharyngoplasty or residual hole closure. Conclusions The epidemiological and clinical profile of the study population resembled the expected distributions in Western populations. The large number of syndromic CL/P and associated comorbidities supports the need for specialized cleft centers and interdisciplinary cleft care.

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Essential role of Msx1 in regulating anterior-posterior patterning of the secondary palate in mice

Cited by 2 publications

References 39 publications

Spatiotemporal Evolution of Developing Palate in Mice

Spatiotemporal Evolution of Developing Palate in Mice

Epidemiological and clinical evaluation of patients with a cleft in lower saxony Germany: a mono-center analysis

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