Essential role of NbNOG1 in ribosomal RNA processing

Abstract

Nucleolar GTP-binding protein 1 (NOG1) is a highly conserved GTPase first reported in Trypanosoma as required for ribosome biogenesis. We characterized NbNOG1, a Nicotiana benthamiana NOG1 ortholog sharing more than 45% amino acid identity with Trypanosoma, yeast, and human NOG1. N. benthamiana plants silenced for NbNOG1 were stunted and produced sterile flowers. NbNOG1 is functionally interchangeable with yeast NOG1 (ScNOG1), rescuing yeast lethality caused by loss of ScNOG1. Finally, NbNOG1 silencing caused over-accumulation of pre-rRNA processing intermediates, and concomitant loss of mature rRNAs. Collectively, these data support a role for NbNOG1 in ribosomal RNA processing.

Ribosome Biogenesis in Plants: From Functional 45S Ribosomal DNA Organization to Ribosome Assembly Factors

“…Moreover, homologs of the yeast Nog1 protein have been reported in Nicotiana benthamiana and Arabidopsisplants. The N. benthamiana homolog of Nog1 is required for processing of 35S, 33S, and 27S intermediates (Guo et al, 2018), while Arabidopsis NOG1-1 and NOG1-2 are involved in biotic and abiotic stress responses (Lee et al, 2017).…”