Essential role of EDHF in the initiation and maintenance of adrenergic vasomotion in rat mesenteric arteries

Mauban, Joseph R. H.; Wier, W. Gil

doi:10.1152/ajpheart.01084.2003

Cited by 44 publications

(57 citation statements)

References 46 publications

(62 reference statements)

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“…In the rat iris arteriole, rabbit ear, and superior mesenteric artery, vasomotion is reported to be endothelium independent (3,18,39), whereas in the rat mesenteric, rabbit femoral artery, and hamster aorta, vasomotion is suggested to be endothelium dependent, as shown by its abolition with endothelium denudation (12,21,34,38,40).…”

mentioning

confidence: 99%

“…In some studies, endothelial cells play an essential role in synchronizing the activity of smooth muscle cells through the release of NO, with endothelium denudation desynchronizing adjacent smooth muscle cell [Ca 2ϩ ] i oscillations, NO inhibition abolishing vasomotion, and exogenous cGMP activating vasomotion (11,30,41). In contrast, in other studies, NO has an apparent inhibitory effect on vasomotion (17,18,20,34,38,48,51,52). Loss of vasomotion and desynchronization of smooth muscle cell [Ca 2ϩ ] i oscillations, which mimic endothelium removal, have also been reported in rat mesenteric artery after incubation in the classical EDHF and calciumactivated potassium channel (K Ca ) blockers apamin and charybdotoxin, thus implying a role for EDHF in vasomotion (8,34,38).…”

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confidence: 99%

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Endothelial coordination of cerebral vasomotion via myoendothelial gap junctions containing connexins 37 and 40

Haddock

Grayson²,

Brackenbury³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

114

116

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Control of cerebral vasculature differs from that of systemic vessels outside the blood-brain barrier. The hypothesis that the endothelium modulates vasomotion via direct myoendothelial coupling was investigated in a small vessel of the cerebral circulation. In the primary branch of the rat basilar artery, membrane potential, diameter, and calcium dynamics associated with vasomotion were examined using selective inhibitors of endothelial function in intact and endothelium-denuded arteries. Vessel anatomy, protein, and mRNA expression were studied using conventional electron microscopy high-resolution ultrastructural and confocal immunohistochemistry and quantitative PCR. Membrane potential oscillations were present in both endothelial cells and smooth muscle cells (SMCs), and these preceded rhythmical contractions during which adjacent SMC intracellular calcium concentration ([Ca 2ϩ ]i) waves were synchronized. Endothelium removal abolished vasomotion and desynchronized adjacent smooth muscle cell [Ca 2ϩ ]i waves. N G -nitro-L-arginine methyl ester (10 M) did not mimic this effect, and dibutyryl cGMP (300 M) failed to resynchronize [Ca 2ϩ ]i waves in endothelium-denuded arteries. Combined charybdotoxin and apamin abolished vasomotion and depolarized and constricted vessels, even in absence of endothelium. Separately, 37,43 Gap27 and 40 Gap27 abolished vasomotion. Extensive myoendothelial gap junctions (3 per endothelial cell) composed of connexins 37 and 40 connected the endothelial cell and SMC layers. Synchronized vasomotion in rat basilar artery is endothelium dependent, with [Ca 2ϩ ]i waves generated within SMCs being coordinated by electrical coupling via myoendothelial gap junctions.connexin; electron microscopy; endothelial function; potassium channel; membrane potential VASOMOTION is an intrinsic feature of many arteries and arterioles under normal and pathological conditions and may contribute to regulation of blood flow and pressure (1,14). Both spontaneous and agonist-induced vasomotion occur when oscillations in the concentration of intracellular calcium ([Ca 2ϩ ] i ) become synchronized among adjacent smooth muscle cells, giving rise to global oscillations in [Ca 2ϩ ] i across the vessel wall (13,15,26,27,33,41,(47)(48)(49).There is clear heterogeneity in the mechanisms that underlie vasomotion, and specifically whether the endothelium plays a mandatory or modulatory role. In the rat iris arteriole, rabbit ear, and superior mesenteric artery, vasomotion is reported to be endothelium independent (3, 18, 39), whereas in the rat mesenteric, rabbit femoral artery, and hamster aorta, vasomotion is suggested to be endothelium dependent, as shown by its abolition with endothelium denudation (12,21,34,38,40).Endothelium-dependent effects on vasomotion have been attributed to the vasodilatory factors nitric oxide (NO) and endothelium-derived hyperpolarizing factor (EDHF). In some studies, endothelial cells play an essential role in synchronizing the activity of smooth muscle cells through the rel...

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mentioning

confidence: 99%

mentioning

confidence: 99%

Endothelial coordination of cerebral vasomotion via myoendothelial gap junctions containing connexins 37 and 40

Haddock

Grayson²,

Brackenbury³

et al. 2006

American Journal of Physiology-Heart and Circulatory Physiology

114

116

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“…Some of these discrepancies can be explained by the modulatory role of ECs on mean SM Ca 2+ and transitions between oscillatory and nonoscillatory domains, similar to the effect of pressure (Mauban and Wier 2004;Koenigsberger et al , 2006. A synchronizing effect of the endothelium through some unidentified mechanism was also suggested by Rahman et al (Rahman et al 2005) based on the finding that cGMP produced only partial synchronization in endothelium denuded RMA, while intact endothelium allowed full synchronization even with L-NAME and ODQ.…”

Section: Role Of Endotheliummentioning

confidence: 93%

“…The effect of endothelium on vasomotion remains controversial and both synchronizing and desynchronizing effects of endothelium have been reported even in the same vessel types, including in RMA (Mauban and Wier 2004;Rahman et al 2005;Sell, Boldt, and Markwardt 2002;Lamboley et al 2003;Aalkjaer and Nilsson 2005). Some of these discrepancies can be explained by the modulatory role of ECs on mean SM Ca 2+ and transitions between oscillatory and nonoscillatory domains, similar to the effect of pressure (Mauban and Wier 2004;Koenigsberger et al , 2006.…”

Section: Role Of Endotheliummentioning

confidence: 99%

“…Some studies suggested that the natural frequencies of SMCs within a vascular segment are very similar and only noise contributes significant variability in the system, whereas others reported that the frequencies of individual SMCs may differ significantly (Koenigsberger et al 2004;Jacobsen et al 2008). Furthermore, the period of vasomotion in RMA varies from few seconds Boedtkjer et al 2008) to almost half a minute (Mauban and Wier 2004;Sell, Boldt, and Markwardt 2002;Seppey et al 2008. In this study, oscillatory frequency was predicted based on previously determined kinetic constants and parameter values, and it falls within the range of previously reported values.…”

Section: Model Limitationsmentioning

confidence: 99%

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Theoretical Investigation of Intra- and Inter-cellular Spatiotemporal Calcium Patterns in Microcirculation

Parikh¹

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Structure and Function of the Microcirculation

Aalkjær

Mulvany

2020

Updates in Hypertension and Cardiovascular Protection

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Essential role of EDHF in the initiation and maintenance of adrenergic vasomotion in rat mesenteric arteries

Cited by 44 publications

References 46 publications

Endothelial coordination of cerebral vasomotion via myoendothelial gap junctions containing connexins 37 and 40

Endothelial coordination of cerebral vasomotion via myoendothelial gap junctions containing connexins 37 and 40

Theoretical Investigation of Intra- and Inter-cellular Spatiotemporal Calcium Patterns in Microcirculation

Structure and Function of the Microcirculation

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