Essential Protective Role of Catalytically Active Antibodies (Abzymes) with Redox Antioxidant Functions in Animals and Humans

Tolmacheva, Anna S.; Nevinsky, Georgy A.

doi:10.3390/ijms23073898

Cited by 14 publications

(29 citation statements)

References 197 publications

(364 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All of these immune system changes and defects increase the proteinuria and the generation of the catalytically active Abs which split DNA, MBP, and MOG [ 6 , 7 , 8 , 9 , 10 , 11 ]. In addition, the development of autoimmune diseases in humans leads to an increase in the concentration of antibodies-abzymes with antioxidant redox catalytic activities [ 37 , 38 , 39 , 40 ]. It showed recently that the development of EAE in autoimmune-prone C57BL/6 mice was associated with an increase in the redox activities of IgGs [ 40 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the development of autoimmune diseases in humans leads to an increase in the concentration of antibodies-abzymes with antioxidant redox catalytic activities [ 37 , 38 , 39 , 40 ]. It showed recently that the development of EAE in autoimmune-prone C57BL/6 mice was associated with an increase in the redox activities of IgGs [ 40 , 48 ]. Those that are prone to the spontaneous development of EAE in Th and 2D2, such as the C57BL/6 mice, demonstrate during the development of the pathology the changes in the profiles of HSCs differentiation and the production of abzymes that hydrolyze DNA, MBP, and MOG [ 6 , 7 , 8 , 9 , 10 , 11 ].…”

Section: Resultsmentioning

confidence: 99%

“…As previously shown, the optimal substrates for the IgGs with redox activities from healthy rats [ 31 , 32 , 33 , 34 , 35 ], healthy donors, patients with autoimmune diseases [ 37 , 38 , 39 , 40 ], and C57BL/6 mice [ 40 , 48 ] are ABTS, DAB, and OPD. We have applied strict criteria to prove that the redox activities of C57BL/6 mice IgGs are their intrinsic properties and to not occur not due to the co-purifying canonical enzymes [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

“…Stable blood IgGs are present in the blood in high concentrations (5.4–18.2 g/L) and remain intact for several months [ 30 ]. It was previously shown that the blood of healthy donors and rats contains antibodies-abzymes with antioxidant peroxidase and oxidoreductase activities [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. In addition, it was demonstrated that abzymes with these redox activities in the blood of MS and SLE patients are statistically higher than they are in healthy donors [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

“…Undeniable evidence that both peroxidase and oxidoreductase catalytic activities are intrinsic to the IgG levels of healthy mammals has been demonstrated using different methods [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ]; for an overview, see [ 40 ]. The redox activities of Abs from healthy volunteers and patients with MS and SLE were compared [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Antibodies-Abzymes with Antioxidant Activities in Two Th and 2D2 Experimental Autoimmune Encephalomyelitis Mice during the Development of EAE Pathology

et al. 2022

Self Cite

View full text Add to dashboard Cite

The exact mechanisms of multiple sclerosis development are still unknown. However, the development of EAE (experimental autoimmune encephalomyelitis) in Th and 2D2 mice is associated with the infringement of the differentiation profiles of bone marrow hematopoietic stem cells which are bound with the production of compounds that are harmful for human autoantibodies-abzymes that hydrolyze myelin oligodendrocyte glycoprotein, myelin basic protein, and DNA. It showed that autoimmune patients’ antioxidant IgG antibodies oxidise some compounds due to their peroxidase (H2O2-dependent) and oxidoreductase (H2O2-independent) activities more effectively than those in healthy humans can. It was interesting to identify whether the redox activities of the antibodies change during the development of autoimmune diseases. Here, we analyzed the change in these redox activities of the IgGs from the blood of Th and 2D2 mice, which corresponded to different stages of the EAE development. The peroxidase activity in the oxidation of ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) in the Th (4-fold) and 2D2 (2-fold) mice IgGs, on average, is higher than the oxidoreductase activity is. The peroxidase activity of the Th (1.9-fold) and 2D2 (3.5-fold) mice IgGs remarkably increased during the 40 days of the spontaneous development of EAE. Forty days after the immunization of the MOG peroxidase activity, the IgGs of the Th and 2D2 mice increased 5.6–6.0 times when they were compared with those that presented no increase (3 months of age). The mice IgGs were oxidized with 3,3′-diaminobenzidine (2.4–4.3-fold) and o-phenylenediamine (139–143-fold) less efficiently than they were with ABTS. However, the temper of the change in the IgG activity in the oxidation of these substrates during the spontaneous and MOG-induced development of EAE was close to that which occurred for ABTS. All of the data show that the IgG peroxidase and oxidoreductase activities of EAE mice can play an important role in their protection from toxic compounds and oxidative stress.

show abstract