Essential oil of Rosmarinus officinalis L. from West Highlands of Algeria: Chemical characterization and in vitro antifungal activity against Fusarium oxysporum f. sp. albedinis

Abstract

Rosmarinus officinalis is a well-studied species; however, R. officinalis essential oil (EO) from West highlands of Algeria was not investigated chemically and biologically. In this context, chemical composition of R. officinalis EOs obtained from leaves and stem were determined by GC/MS analysis and their antifungal activity against Fusarium oxysporum f. sp. albedinis (Foa) were evaluated. The GC/MS analysis indicated that monoterpenes were the dominant class of compounds in both leaves and stem (oxygenated 84.9%, 52.4%) and hydrocarbons (12.2%, 8.4 %), respectively. Among them, 1,8-cineole was the main component (leaves: 54.4%, stem: 29.7%), which classifies these EOs as 1,8-cineole chemotype. The in vitro antifungal activity of Eos was evaluated through micro-atmosphere and direct contact methods. Best inhibitory activity against Foa was determined after 7-day incubation using direct contact method by relative growth reduction (RGR= 0.398; RGR=0.383) with EOs from leaves and stem, respectively.

Chemical Variability and Anti‐Inflammatory Activity of Rosmarinus officinalis L. Leaf Essential Oil from Algerian Sahara

“…For comparison with previous works relative to Algerian R. officinalis , cineole‐rich essential oils have been reported from plants harvested in Bordj Bou Arreridj (South‐East Algiers), [7,26] Béjaïa and Tébessa (Eastern Algeria), [26,28] El‐Bayadh, [21,29] Khenchela [30] . Camphor‐rich EOs have been isolated from plants collected in Batna and M'Sila, [26] Constantine (North‐East Algeria), [36] Djelfa and Laghouat [23] .…”

“…[7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] A few papers concerned 2-6 oil samples. [23,[24][25][26][27][28][29][30] Lastly, a study investigated the composition of 15 oil samples, however no statistical analysis was performed. [31] Otherwise, various studies concerned the composition as a function of the plant life cycle, [32] and the isolation of rosemary oils by different processes.…”

“…In short, essential oil samples could be divided into two main groups. The first group contains all the essential oil samples whose composition is strongly dominated by a major component (with a content at least twice that of the second component) i) 1,8cineole (30-73 %), [7][8][9]20,21,23,26,[28][29][30][31]33,34] ii) α-pinene (28.5-51.8 %), [27,31,35] iii) camphor (33.1-52.1 %). [23,26,31,36] The remaining oil samples displayed two-three components with more or less similar contents i) α-pinene/cineole/camphor; [7,11,12,14,18,24,26,33] ii) α-pinene/camphene/camphor; [19,26,27,30,31] iii) α-pinene/ camphor; [24,27] iv) cineole/camphor; [10,22,25,33] v) miscellaneous.…”

“…However, most papers reported the chemical composition of a unique oil sample, mostly harvested on the wild and scarcely cultivated [7–22] . A few papers concerned 2–6 oil samples [23,24–30] . Lastly, a study investigated the composition of 15 oil samples, however no statistical analysis was performed [31] .…”