Essential oil of Algerian Eucalyptus citriodora : Chemical composition, antifungal activity

Tolba, Hadjer; Moghrani, Houria; Benelmouffok, A; Kellou, D.; Maachi, Rachida

doi:10.1016/j.mycmed.2015.10.009

Cited by 67 publications

(62 citation statements)

References 16 publications

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“…The antifungal activity of terpenoids, one of the major groups of volatile secondary metabolites, is known in the pharmaceutical field [17]. Thus, the antifungal activity of the EO in this present study may be related to the monoterpenes identified in the GC-MS assay.…”

Section: Resultsmentioning

confidence: 62%

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Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species

Toledo

Ramos

Spósito

et al. 2016

IJMS

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Background: The incidence of fungal infections, especially those caused by Candida yeasts, has increased over the last two decades. However, the indicated therapy for fungal control has limitations. Hence, medicinal plants have emerged as an alternative in the search for new antifungal agents as they present compounds, such as essential oils, with important biological effects. Published data demonstrate important pharmacological properties of the essential oil of Cymbopogon nardus (L.) Rendle; these include anti-tumor, anti-nociceptive, and antibacterial activities, and so an investigation of this compound against pathogenic fungi is interesting. Objective: The aim of this study was to evaluate the chemical composition and biological potential of essential oil (EO) obtained from the leaves of C. nardus focusing on its antifungal profile against Candida species. Methods: The EO was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Testing of the antifungal potential against standard and clinical strains was performed by determining the minimal inhibitory concentration (MIC), time-kill, inhibition of Candida albicans hyphae growth, and inhibition of mature biofilms. Additionally, the cytotoxicity was investigated by the IC50 against HepG-2 (hepatic) and MRC-5 (fibroblast) cell lines. Results: According to the chemical analysis, the main compounds of the EO were the oxygen-containing monoterpenes: citronellal, geranial, geraniol, citronellol, and neral. The results showed important antifungal potential for all strains tested with MIC values ranging from 250 to 1000 μg/mL, except for two clinical isolates of C. tropicalis (MIC > 1000 μg/mL). The time-kill assay showed that the EO inhibited the growth of the yeast and inhibited hyphal formation of C. albicans strains at concentrations ranging from 15.8 to 1000 μg/mL. Inhibition of mature biofilms of strains of C. albicans, C. krusei and C. parapsilosis occurred at a concentration of 10× MIC. The values of the IC50 for the EO were 96.6 μg/mL (HepG-2) and 33.1 μg/mL (MRC-5). Conclusion: As a major virulence mechanism is attributed to these types of infections, the EO is a promising compound to inhibit Candida species, especially considering its action against biofilm.

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Section: Resultsmentioning

confidence: 62%

“…The presence of terpenes, as one of the chemical compounds in EO, contributes to the complex constitution with the action against microorganisms being directly related to this characteristic [17]. …”

Section: Introductionmentioning

confidence: 99%

Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species

Toledo

Ramos

Spósito

et al. 2016

IJMS

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“…The Analysis of Variance (ANOVA) indicated that the essential oils yields were significantly different between species ( P <0.05) with the presence of three non‐overlapping groups. The comparative study of our results to those obtained by other researchers showed that E. citriodora grown in Choucha arboreta (Tunisia) is much richer in essential oil than those obtained from Australia, Uruguay, Congo, Morocco, Algeria and Tunisia (Zerniza arboreta) with an essential oil yield inferior to 3.3±0.1 %. E. alba leaves were also richer in essential oils than those obtained from Nigeria (0.28 %), Congo (0.22 %), Burkinafaso (1.2 %), Bangladesh (0.85 %), and Senegal (0.6–0.9 %) .…”

Section: Resultsmentioning

confidence: 99%

Chemical Characterization of Five TunisianEucalyptusEssential Oils Species

Elaissi

Moumni

Roeleveld

et al. 2019

Chemistry & Biodiversity

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Hydrodistillation of the dried leaves of five Eucalyptus species, E. alba REINW. ex BLUME, E. citriodora HOOK., E. paniculata SM., harvested from Choucha arboreta (region of Sejnane, northwest of Tunisia), E. pimpiniana MAIDEN from Mjez Elbab arboreta (north east of Tunisia) and E. bicolor A.CUNN ex HOOK from Sidi Smail arboreta (center of Tunisia), in March 2017, afforded essential oils in yields varying from 1.3 � 0.2 to 6.0 � 0.9 % according to the species. E. citriodora provided the highest mean percentage of essential oil amongst all the species. Analysis by GC (RI) and GC/MS allowed the identification of 138 components representing 84.6 -98.7 % of the total oil. The content of the different samples varied according to the species. The main components were citronellol, followed by 1, 8-cineole, α-pinene, τ-cadinol, 7-epi-α-eudesmol, trans-pinocarveol, spathulenol, aromadendrene, γ-cadinene and δ-cadinene. The principal components and the hierarchical cluster analyses separated the five leaf essential oils into three groups, each group constituted a chemotype.

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“…The definition of the MIC differs between various publications [57,58,59], so it was decided that the MIC would be defined as the lowest volume of essential oil that inhibited the growth of the tested fungi with an inhibition rate higher than 80%. The MIC is expressed in different units including mg/mL [60], µg/mL [61,62], µL/L [63,64], µL/mL [65], and ppm [62,66]. In this study, the MIC of fungi was expressed as ppm.…”

Section: Methodsmentioning

confidence: 99%

Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

et al. 2017

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The antifungal, antibacterial, and antioxidant activity of four commercial essential oils (EOs) (thyme, clove, rosemary, and tea tree) from Romanian production were studied in order to assess them as bioactive compounds for active food packaging applications. The chemical composition of the oils was determined with the Folin–Ciocâlteu method and gas chromatography coupled with mass spectrometry and flame ionization detectors, and it was found that they respect the AFNOR/ISO standard limits. The EOs were tested against three food spoilage fungi—Fusarium graminearum, Penicillium corylophilum, and Aspergillus brasiliensis—and three potential pathogenic food bacteria—Staphylococcus aureus, Escherichia coli, and Listeria monocytogenes—using the disc diffusion method. It was found that the EOs of thyme, clove, and tea tree can be used as antimicrobial agents against the tested fungi and bacteria, thyme having the highest inhibitory effect. Concerning antioxidant activity determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) methods, it has been established that the clove oil exhibits the highest activity because of its high phenolic content. Promising results were obtained by their incorporation into chitosan emulsions and films, which show potential for food packaging. Therefore, these essential oils could be suitable alternatives to chemical additives, satisfying the consumer demand for naturally preserved food products ensuring its safety.

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Essential oil of Algerian Eucalyptus citriodora : Chemical composition, antifungal activity

Cited by 67 publications

References 16 publications

Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species

Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species

Chemical Characterization of Five TunisianEucalyptusEssential Oils Species

Comparative Analysis of the Composition and Active Property Evaluation of Certain Essential Oils to Assess their Potential Applications in Active Food Packaging

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