Essential oil-in-water microemulsions for topical application: structural study, cytotoxic effect and insect repelling activity

Chatzidaki, Maria D.; Demisli, Sotiria; Zingkou, Eleni; Liggri, Panagiota G.V.; Papachristos, Dimitrios; Balatsos, George; Karras, Vasilios; Nallet, Frédéric; Michaelakis, Αntonios; Sotiropoulou, Georgia; Zographos, Spyros E.; Papadimitriou, Vassiliki

doi:10.1016/j.colsurfa.2022.130159

Cited by 6 publications

(2 citation statements)

References 49 publications

(53 reference statements)

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“…The viscosity observed in the optimized KME formulation aligned with the typical characteristic of microemulsions, which is low viscosity. This characteristic contributed to the ease of handling, spreading, and absorption of microemulsions, making it suitable for topical applications [39]. Additionally, the zeta potential can predict the physical stability of nanoformulations.…”

Section: Antifungal Activitymentioning

confidence: 99%

Approach for Development of Topical Ketoconazole-Loaded Microemulsions and Its Antifungal Activity

Naksuriya,

Nitthikan,

Supadej

et al. 2023

Trends Sci

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Loading ketoconazole in microemulsions can enhance the solubility and permeation of ketoconazole into the skin. The systematic approach as quality by design (QbD) can help for better product and process understanding. This study aimed to develop topical ketoconazole-loaded microemulsions (KME) based on the QbD approach. After risk assessment, the design of experiment was utilized to determine the optimal ratio of water, isopropyl myristate, and surfactant mixture (polysorbate 80 and ethanol), which were chosen as critical material attributes. The particle size and polydispersity index, selected as critical quality attributes, were evaluated as responses. Then, characterizations, in vitro permeation, cytotoxicity, and antifungal activity studies were tested. As result, the equations from the D-optimal mixture design model successfully predicted the composition of the optimized formulation and obtained the acceptable design space. The optimized KME was 16.00 % w/w of isopropyl myristate, 73.50 % w/w of surfactant mixture, and 8.50 % w/w of water resulted in a water-in-oil system. The particle size and polydispersity index of KME were 21.1 ± 1.6 nm and 0.329 ± 0.020, respectively. KME showed prolonged skin retention after 24 h and was non-toxic. KME exhibited a broader zone of inhibition and lower inhibition concentration compared to unloaded ketoconazole against dermatophytes, indicating that KME enhanced effectiveness in antifungal activity for skin infection. The results of the KME meeting the quality target indicated a well-designed and suitable microemulsion for topical usage. Therefore, employing QbD in identifying the process and formulation is a promising approach for future development of high-quality topical KME products. HIGHLIGHTS The topical ketoconazole-loaded microemulsions (KME) were developed based on quality by design approach for further industrial production The D-optimal mixture design model successfully predicted the design space of desired KME formulation KME showed prolonged skin retention after 24 h KME was non-toxic for a topical dosage form KME exhibited antifungal activity greater than unloaded ketoconazole GRAPHICAL ABSTRACT

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Section: Antifungal Activitymentioning

confidence: 99%

Approach for Development of Topical Ketoconazole-Loaded Microemulsions and Its Antifungal Activity

Naksuriya,

Nitthikan,

Supadej

et al. 2023

Trends Sci

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“…In recent years, nano-dispersions such as nanoemulsions or microemulsions have been increasingly used in advanced cosmetics from sun-screens to insect repellents due to their ability to offer increased stability and the superior delivery of active ingredients compared to traditional macroemulsions [11,12]. More specifically, microemulsions are thermodynamically stable liquid mixtures containing oil, water, and amphiphilic molecules that serve as the interfacial area between the two immiscible phases [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Nanotechnology for Effective Epilation: Assessment of the Application of a Protease-Containing Microemulsion

Skórka,

Gahrtz,

Chatzidaki

et al. 2024

Cosmetics

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Background: Epilation is a very effective way to remove unwanted hair because of its long-lasting effects. However, there are some disadvantages such as pain during the procedure, the possibility of ingrown hairs and perifollicular inflammation. In the present study, we investigated whether a protease-containing post-epilation microemulsion is effective in improving epilation performance and alleviating the above problems. Methods: The application characteristics and effects of the tested microemulsion were evaluated during and after three applications in 30 female volunteers. This was conducted by measuring hair density, assessing hair strength, and evaluating the subjective experience of the volunteers using a questionnaire. Results: The measurements showed that after three applications, the apparent hair density in the axilla was reduced from 43.89 ± 12.44 hairs/cm2 to 16.67 ± 6.61 hairs/cm2 (p < 0.0001). In general, volunteers observed a reduced hair regrowth rate, resulting in longer epilation intervals, and more soothed and moisturized skin. Volunteers who previously experienced ingrown hairs or perifollicular inflammation reported the absence or improvement of these problems. Conclusions: The protease-containing microemulsion not only improves the performance of the epilation procedure, leading to less frequent epilation, but also improves possible negative effects of epilation such as ingrown hairs and perifollicular inflammation.

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Soft nanostructures for sun protection formulations

Xenakis,

Galani,

Papadimitriou

et al. 2024

Current Opinion in Colloid & Interface Science

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Essential oil-in-water microemulsions for topical application: structural study, cytotoxic effect and insect repelling activity

Cited by 6 publications

References 49 publications

Approach for Development of Topical Ketoconazole-Loaded Microemulsions and Its Antifungal Activity

Approach for Development of Topical Ketoconazole-Loaded Microemulsions and Its Antifungal Activity

Nanotechnology for Effective Epilation: Assessment of the Application of a Protease-Containing Microemulsion

Soft nanostructures for sun protection formulations

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