Essential oil from Sabina chinensis leaves: A promising green control agent against Fusarium sp.

Abstract

Sabina chinensis is a woody plant with important ecological functions in different regions of China, but its essential oils (EO) against plant pathogenic fungi remain largely undetermined. The purpose of our study was to assess the chemical composition and antifungal activity of S. chinensis EO based on optimization of the extraction process. In this study, an actionable and effective model with the experimental results and identified optimum conditions (crushing degree of 20 mesh, liquid–solid ratio of 10.1:1, immersion time of 9.1 h) was established successfully to achieve an extraction yield of 0.54%, which was basically consistent with the theoretical value. A total of 26 compounds were identified using headspace gas chromatography–mass spectrometry (GC–MS) and showed that the major constituent was β-phellandrene (26.64-39.26%), followed by terpinen-4-ol (6.53-11.89%), bornyl acetate (6.13-10.53%), etc. For Petri plate assays, our experiments found for the first time that S. chinensis EO revealed high and long-term antifungal activity against the tested strains, including Fusarium oxysporum and Fusarium incarnatum, at EC50 values of 1.42 and 1.15 µL/mL, which especially reached approximately 76% and 90% growth inhibition at a dose of 0.2 µL/mL, respectively. Furthermore, the antifungal activity of EO from different harvest periods showed remarkable variation. The orthogonal partial least-squares discriminant analysis (OPLS-DA) method revealed 11 metabolites with chemical marker components, and 5 of its potential antifungal activities, terpinen-4-ol, α-terpineol, α-elemol, γ-eudesmol, and bornyl acetate, were strongly correlated with the mycelial inhibition rate. In total, this study explored the antifungal activity of EO against root rot fungus as a potential fungicide and provided valuable information into developing potential products from natural agents.

Antifungal Activity of Thyme and Clove Essential Oil Nanoemulsions against Pothos Root Rot

“…ciceris, and Macrophomina phaseolina. Our observations also agree with the results reported by Zhang et al (2022), who found that Sabina chinensis essential oil exhibited potent antifungal activity against F. oxysporum and F. incarnatum. The beneficial effects of the investigated oils on promoting vegetative development involving root length, root fresh weight, and root dry weight (Fig.…”

“…ciceris, and Macrophomina phaseolina. Also, Zhang et al (2022) reported that Sabina chinensis essential oil exhibited potent antifungal activity against F. oxysporum and F. incarnatum. However, a number of concerns about the high volatility, degradability, photosensitivity, destabilization, and wettability of essential oils restrict their use in the field (Song et al, 2022).…”

Coconut Juice Enhances Rooting and Leaf Essential Oils of Juniperus sabina L. Cuttings

“…In contrast, in the study of Ghorbanzadeh et al [72], high levels of myrtenyl acetate were detected in J. sabina tissue; however, this terpenoid was absent in both of the other studied species and has not been reported in other studies focusing on juniper. The study of Zhang et al [7] in Juniperus chinensis showed that the major constituent was beta-phellandrene (26.64%-39.26%), followed by terpinen-4ol (6.53%-11.89%). Therefore, the type of compound and its importance in the total oil produced varies greatly depending on the site.…”

“…The resilience and hardiness of junipers make them suitable for reforestation in less productive or degraded areas [1]. The Juniperus genus also includes important groups of species from which to obtain EOs because the number of aromatic elements is very high [3][4][5][6][7].…”

Phytochemistry and Pharmacology of Sesquiterpenoids from Atractylodes DC. Genus Rhizomes

“…A chemical investigation of the ethanol extract of A. lancea resulted in the isolation of nine eudesmanetype sesquiterpenoids (41)(42)(43)(44)(45)(46)(47)(48)(49), and their structures were elucidated using spectroscopic techniques and HRESIMS [47]. Kamauchi et al [48] gained two new eudesmane-type sesquiterpenoids, namely 3α-hydroxy pterocarpol (50) and (11R)-2,11,12-trihydroxy-bselinene (51), along with three known sesquiterpenoids (52)(53)(54) in the fermented rhizomes of A. lancea using column chromatography. β-eudesmol (55) and atractylon (98) were widely distributed in genus species [26,[49][50][51][52].…”