Essential Oil from Myrtle Leaves Growing in the Eastern Part of Saudi Arabia: Components, Anti-inflammatory and Cytotoxic Activities

Mohamed, Maged E.; Mohafez, Omar Mahmoud Mohamed; Khalil, Hany Ezzat; Alhaider, Ibrahim A.

doi:10.1080/0972060x.2019.1645046

Cited by 11 publications

(11 citation statements)

References 42 publications

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“…This test has been used to evaluate the cytotoxicity of the myrtle EO in antimalarial assays [ 68 ] and to evaluate some biological properties, including the cytotoxicity of EOs from different areas of Algeria [ 69 ], Morocco [ 21 ], or Saudi Arabia [ 70 ]. The study performed by Alves and coworkers on thyme EOs demonstrated their ability to inhibit the biofilm formation and their influence on the viability of the cells entrapped within the biofilm, so to avoid or limit the subsequent cell changes occurring in [ 71 ]. The myrtle EO has been tested to inhibit the bacterial quorum-sensing mechanism [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

Chemical composition, antibiofilm, cytotoxic, and anti-acetylcholinesterase activities of Myrtus communis L. leaves essential oil

Caputo

Capozzolo

Amato

et al. 2022

BMC Complement Med Ther

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Background The potential of essential oils (EOs) and of their principal constituents for eradication of biofilm and at the same time the research of new potential acetylcholinesterase inhibitors is gaining increasing interest in last years. The aims of this study were to determine the chemical composition and to evaluate the antibacterial, cytotoxic, and anti-acetylcholinesterase properties of Myrtus communis leaves essential oil and its main constituents. Methods Essential oil was obtained by hydrodistillation of M. communis L. leaves and was analyzed by GC and GC–MS. The antimicrobial activity was carried out against both gram-negative and gram-positive bacteria. The microdilution method was used to estimate the minimum inhibitory concentrations (MICs). Then, the capacity of essential oil and its main constituent to inhibit biofilm growth, with the method of O’Toole and Kolterand, and the metabolic activity of biofilm cells through the MTT colorimetric method were evaluated at different times. Moreover, was studied the potential cytotoxic activity against SH-SY5Y cell line with MTT assay and the anti-acetylcholinesterase activity using Ellman’s assay. Results Myrtenyl-acetate, 1,8 cineole, α-pinene, and linalool were the main components in the EO. The myrtle EO, at the minimum tested dose (0.4 mg/ml), inhibited S. aureus biofilm by 42.1% and was capable of inhibiting the biofilm cell metabolism in all tested strains, except Staphylococcus aureus. Moreover, the EO showed good cytotoxic and anti-acetylcholinesterase activities IC50 of 209.1 and 32.8 μg/ml, respectively. Conclusions The results suggest that myrtle EO and its main constituents could be used as possible products that could act against the resistant pathogenic species E. coli, P. aeruginosa, L. monocytogenes and S. aureus, on the other hand, as possible coadjutants in the treatment of neurological diseases.

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Section: Discussionmentioning

confidence: 99%

Chemical composition, antibiofilm, cytotoxic, and anti-acetylcholinesterase activities of Myrtus communis L. leaves essential oil

Caputo

Capozzolo

Amato

et al. 2022

BMC Complement Med Ther

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“…One hundred grams of the carefully collected and air-dried whole A. lanatum plant were subjected to hydro-distillation using a Clevenger-type apparatus for 3 h. EO (yield 0.46% volume per dried plant weight) was recovered and dried over anhydrous sodium sulphate. The EO sample was kept in amber-colored vials in a refrigerator at 4 °C until further analyses [ 4 ].…”

Section: Methodsmentioning

confidence: 99%

“…Essential oils (Eos), as secondary metabolites produced from such medicinal and aromatic plants, offer great value in terms of their various curative and biological properties. Several investigations have demonstrated the anti-inflammatory, anti-oxidant, anti-fungal, anti-microbial, and cytotoxic activities of such Eos [ 2 , 3 , 4 ]. Cancer is described as a fatal health condition, considered to be one of major factors leading to death.…”

Section: Introductionmentioning

confidence: 99%

Insight into Analysis of Essential Oil from Anisosciadium lanatum Boiss.—Chemical Composition, Molecular Docking, and Mitigation of Hepg2 Cancer Cells through Apoptotic Markers

Khalil

Ibrahim

Darrag

et al. 2021

Plants

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Essential oils have been used in various traditional healing systems since ancient times worldwide, due to their diverse biological activities. Several studies have demonstrated their plethora of biological activities—including anti-cancer activity—in a number of cell lines. Anisosciadium lanatum Boiss. is a perennial aromatic herb. Traditionally, it is an edible safe herb with few studies exploring its importance. The current study aims to investigate the chemical composition of essential oil isolated from Anisosciadium lanatum using GC-MS, as well as report its anti-cancer potential and its mechanistic effect on HepG2 liver cancer cell lines, and conduct molecular docking studies. To achieve this, the essential oil was isolated using a Clevenger apparatus and analyzed using GC-MS. The cell viability of HepG2 liver cancer and normal fibroblast NIH-3T3 cell lines was assessed by MTT cytotoxicity assay. The effects of the essential oil on cell migration and invasion were assessed using wound healing and matrigel assays, respectively. The effect of the essential oil on migration and apoptotic-regulating mRNA and proteins was quantified using quantitative real-time PCR and Western blot techniques, respectively. Finally, computational docking tools were used to analyze in silico binding of major constituents from the essential oil against apoptotic and migration markers. A total of 38 components were identified and quantified. The essential oil demonstrated regulation of cell proliferation and cell viability in HepG2 liver cancer cells at a sub-lethal dose of 10 to 25 μg/mL, and expressed reductions of migration and invasion. The treatment with essential oil indicated mitigation of cancer activity by aborting the mRNA of pro-apoptotic markers such as BCL-2, CASPASE-3, CYP-1A1, and NFκB. The algorithm-based binding studies demonstrated that eucalyptol, nerol, camphor, and linalool have potent binding towards the anti-apoptotic protein BCL-2. On the other hand, camphor and eucalyptol showed potent binding towards the pro-apoptotic protein CASPASE-3. These findings highlight the effectiveness of the essential oil isolated from Anisosciadium lanatum to drive alleviation of HepG2 cancer cell progression by modulating apoptotic markers. Our findings suggest that Anisosciadium lanatum could be used as a phytotherapeutic anti-cancer agent, acting through the regulation of apoptotic markers. More well-designed in vivo trials are needed in order to verify the obtained results.

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“…Myrtle essential oil showed a notable inhibitory effect on T47D cell lines’ proliferation. Their results demonstrated that myrtle essential oil could be a promising agent in complementary therapy [ 35 ].…”

Section: Phytochemical Studiesmentioning

confidence: 99%

Myrtle: a versatile medicinal plant

Gorjian

Khaligh

2023

Nutrire

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Myrtus, commonly called myrtle, is a genus of flowering plants in the Myrtaceae family. This study aimed to review myrtle’s pharmaceutical, food, and other uses. The pharmacological effects of myrtle for antioxidant, antibacterial, and anti-inflammatory activities, reduction of COVID-19 symptoms, anti-diabetic in the animal model, hepatoprotective in the rat model, antihypertensive, control of intestinal helminthiasis in mice model, inhibition of glucosyltransferase activity, protective effect on oxidative metabolism in the hypothyroidism model, and reducing the damage caused by skin burns are reviewed. In addition, the food uses of this plant such as improving the oxidative and microbial stability of products containing salmon, antimicrobial activity in meat and dairy products, flavoring in sea salt, microbial improvement of fresh fruits during post-harvest storage, animal nutrition, and bio-oil production are summarized. Supplementary Information The online version contains supplementary material available at 10.1186/s41110-023-00194-y.

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Essential Oil from Myrtle Leaves Growing in the Eastern Part of Saudi Arabia: Components, Anti-inflammatory and Cytotoxic Activities

Cited by 11 publications

References 42 publications

Chemical composition, antibiofilm, cytotoxic, and anti-acetylcholinesterase activities of Myrtus communis L. leaves essential oil

Chemical composition, antibiofilm, cytotoxic, and anti-acetylcholinesterase activities of Myrtus communis L. leaves essential oil

Insight into Analysis of Essential Oil from Anisosciadium lanatum Boiss.—Chemical Composition, Molecular Docking, and Mitigation of Hepg2 Cancer Cells through Apoptotic Markers

Myrtle: a versatile medicinal plant

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