Essential Oil Composition of Greek (Origanum vulgaressp.hirtum) and Turkish (O. onites) Oregano: a Tool for Their Distinction

Kokkini, Stella; Karousou, Regina; Hanlidou, Effie; Lanaras, T.

doi:10.1080/10412905.2004.9698735

Cited by 57 publications

(38 citation statements)

References 28 publications

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“…Moreover, this well known aromatic herb is endowed by medicinal properties due to its high content of carvacrol, thymol and other phenolic compounds. In this respect, it finds interesting applications in pharmacology . In addition, oregano contains notable quantities of two triterpenic acids, ursolic acid and oleanolic acid, in their free forms.…”

Section: Introductionmentioning

confidence: 99%

“…Several analytical methods have been used in the past for the detection and quantification of the oregano constituents including gas and liquid chromatography, mass spectrometry and NMR spectroscopy . The application of two‐dimensional (2D) NMR spectroscopy to oregano extracts succeeded in detecting the phenolic compounds o ‐coumaric acid, ferulic acid, caffeic acid, p ‐hydroxybenzoic acid and vanillic acid.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by ³¹P NMR spectroscopy

Agiomyrgianaki

Dais

2012

Magnetic Reson in Chemistry

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(31)P nuclear magnetic resonance (NMR) spectroscopy was used to detect and quantify simultaneously a large number of phenolic compounds and the two triterpenic acids, ursolic acid and oleanolic acid, extracted from two oregano species Origanum onites and Origanum vulgare ssp. Hirtum using two different organic solvents ethanol and ethyl acetate. This analytical method is based on the derivatization of the hydroxyl and carboxyl groups of these compounds with the phosphorous reagent 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxa phospholane and the identification of the phosphitylated compounds on the basis of the (31)P chemical shifts. Unambiguous assignment of the (31)P NMR chemical shifts of the dihydroxy- and polyhydroxy-phenols in oregano species as well as those of the triterpenic acids was achieved upon comparison with the chemical shifts of model compounds assigned by using two-dimensional NMR techniques. Furthermore, the integration of the appropriate signals of the hydroxyl derivatives in the corresponding (31)P NMR spectra and the use of the phosphitylated cyclohexanol as an internal standard allowed the quantification of these compounds. The validity of this technique for quantitative measurements was thoroughly examined.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by ³¹P NMR spectroscopy

Agiomyrgianaki

Dais

2012

Magnetic Reson in Chemistry

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“…Monoterpenoids have been considered as the main active and characteristic compounds of the essential oil of O. onites ( Table ). Since they have excellent activity potentials, phytochemical studies have focused particularly on these compounds . In the majority of studies, carvacrol ( 32 ) (2.04 – 92.66%), thymol ( 89 ) (0.18 – 80.44%), linalool ( 56 ) (0.03 – 50.53%), γ ‐terpinene ( 21 ) (0.01 – 18.80%), α ‐terpinene ( 20 ) (0.10 – 15.46%), terpinen‐4‐ol ( 85 ) (0.12 – 15.18%), p ‐cymene ( 6 ) (0.61 – 13.97), β ‐bisabolene ( 99 ) (0.10 – 18.91%), α ‐pinene ( 16 ) (0.02 – 10.33%), borneol ( 26 ) (0.08 – 9.00%), myrcene ( 13 ) (0.06 – 6.33%), α ‐thujene ( 23 ) (0.03 – 6.13%), β ‐caryophyllene ( 104 ) (0.01 – 5.56%), β ‐pinene ( 17 ) (0.01 – 5.40%), camphene ( 1 ) (0.01 – 4.63%), α ‐terpineol ( 82 ) (0.10 – 4.59%), caryophyllene oxide ( 128 ) (0.02 – 2.57), terpinolene ( 22 ) (0.05 – 2.17%), limonene ( 12 ) (0.01 – 1.45%), and α ‐phellendrane ( 14 ) (0.01 – 1.09%) %) have been determined as the main compounds.…”

Section: Phytochemistrymentioning

confidence: 99%

Medicinal Uses, Phytochemistry, and Pharmacology of Origanum onites (L.): A Review

Tepe

Çakır

Tepe

2016

Chemistry & Biodiversity

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Origanum onites L., known as Turkish oregano, has great traditional, medicinal, preservative, and commercial importance. It is used for the treatment of several kinds of ailments, such as gastrointestinal disorders, diabetes, high cholesterol, leukemia, bronchitis, etc. In this review, traditional use, phytochemistry, and pharmacology of O. onites reported between 1988 and 2014 were discussed. This review was prepared based on literature survey on scientific journals and books from libraries and electronic sources, such as Web of Science, PubMed, Scopus, Google Scholar, etc. All databases were searched up to June 2014. Several different classes of terpenoids, triterpene acids, phenolic acids, hydroquinones, flavonoids, hydrocarbons, sterols, pigments, fatty acids, tocopherols, and inorganic compounds were detected mainly in the aerial parts of this plant. Pharmacological studies revealed that extracts obtained from several solvents and individual compounds exhibited antimicrobial, antiviral, antioxidant, insecticidal, anticancer, hepatoprotective, genotoxic, antidiabetic, cholinesterase inhibitory, anti-inflammatory, analgesic activities, etc. O. onites, in general, exhibited remarkable activity potential in almost all test systems. The results of toxicity studies indicated that O. onites did not show any significant toxicity and mutagenicity on Drosophila and Salmonella. Toxicity of the extracts/essential oils and also individual compounds should be evaluated on mammalian cells to ensure their safety. The bioactivity of individual compounds aside from terpenoids should also be assessed in detail. Additionally, mode of action for the bioactive compounds should be evaluated to understand the complex pharmacological effects of these phytochemicals.

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“…The antimicrobial and antioxidant activities of oregano have been attributed to these compounds, which can be present in different proportions, depending on the species of oregano under study, as well as on the climate and growing conditions (Kokini et al . ; Davidson and Taylor ). Most of the antimicrobial studies have been performed with pathogenic microorganisms, and there is not much information about food spoilage molds (Portillo‐Ruiz et al .…”

Section: Introductionmentioning

confidence: 97%

Effect of Mexican Oregano (L ippia berlandieri Schauer) Essential Oil Fractions on the Growth of A spergillus spp. in a Bread Model System

Avila-Sosa

Portillo-Ruiz

Viramontes-Ramos

et al. 2014

Journal of Food Processing and Preservation

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Spices acting as antimicrobial agents can be used in combination with other factors to assure food safety. The aim of this work was to evaluate the effect of Mexican oregano (Lippia berlandieri Schauer) essential oil (EO) fractions on the growth of Aspergillus spp. using a bread model media. Fungal growth was evaluated on a wheat flour-based medium with controlled Aw (0.925, 0.950) and with five Mexican oregano EO fractions with different concentrations of thymol and carvacrol (50-200 mg/kg). Fungal growth (modified Gompertz equation) was affected by Aw values and the concentration of Mexican oregano EO fractions. Differences on thymol and carvacrol had no significant effect on the fungal growth kinetic parameters. Low Aw (0.925) and fractions with high concentration of carvacrol (1 and 2) at 100 mg/kg showed fungicidal activity. Combined methods for food preservation can include Mexican oregano EO, where Aspergillus spp. fungal growth can be inhibited. PRACTICAL APPLICATIONSIncorporation of herbs and essential oils to food products can be considered as a good form to increase shelf life of the product, but usually data on antimicrobial and/or antioxidant capacity are performed under laboratory-controlled conditions. The development of predictive models that consider the effect of the food components on the effectiveness of natural antimicrobials can lead to a better understanding of the best conditions for their addition to food products. The present work describes the effect of water activity and wheat components on the antifungal activity of Mexican oregano essential oil. The composition of the essential oil and its concentration were tested in combination with water activity, and it was observed that those factors greatly affected the biological activity of the essential oil.

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Essential Oil Composition of Greek (Origanum vulgaressp.hirtum) and Turkish (O. onites) Oregano: a Tool for Their Distinction

Cited by 57 publications

References 28 publications

Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by ³¹P NMR spectroscopy

Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by ³¹P NMR spectroscopy

Medicinal Uses, Phytochemistry, and Pharmacology of Origanum onites (L.): A Review

Effect of Mexican Oregano (L ippia berlandieri Schauer) Essential Oil Fractions on the Growth of A spergillus spp. in a Bread Model System

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Essential Oil Composition of Greek (Origanum vulgaressp.hirtum) and Turkish (O. onites) Oregano: a Tool for Their Distinction

Cited by 57 publications

References 28 publications

Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by 31P NMR spectroscopy

Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by 31P NMR spectroscopy

Medicinal Uses, Phytochemistry, and Pharmacology of Origanum onites (L.): A Review

Effect of Mexican Oregano (L ippia berlandieri Schauer) Essential Oil Fractions on the Growth of A spergillus spp. in a Bread Model System

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Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by ³¹P NMR spectroscopy

Simultaneous determination of phenolic compounds and triterpenic acids in oregano growing wild in Greece by ³¹P NMR spectroscopy