Essential oil composition and biological activities of Hyssopus officinalis and Perilla frutescens

Abstract

This article reports on the main constituents of Hyssopus officinalis and Perilla frutescens essential oils (EOs) and their activity as antioxidant, antimicrobial, anti inflammatory and anticancer. The major component of H. officinalis essential oil was isopinocamphone followed by pinocamphone, β-pinene, β-phellandrene, α-thujene and bicyclo-germacrene. The main constituent of the essential oil of P. frutescens was identified as L-perillaldehyde followed by trans-caryophyllene and D-limonene. Both essential oils (hyssop and perilla) showed antioxidant activities with the used three methods and the increase of activity was concentration dependent up to 32μg/ml comparing to ascorbic acid. The values of IC50 for DPPH radical scavenging were in the order of P. frutescens< ascorbic acid< H. officinalis. The values of IC50 for FIC method were in the order of ascorbic acid< P. frutescens< H. officinalis. The IC50 for ABTS method was 8μg for P. frutescence and the values were in the order as ascorbic acid< H. officinalis< P. frutescence. The essential oil of both plant species showed variable activities against all the tested bacterial. The results displayed also that the essential oil of P. frutescens showed significant fungal inhibitions against the four tested Candida strains.

Chemical composition of essential oils of Lavandula angustifolia and Lavandula hybrida cultivated in Egypt and their biological activities

“…These results are in agreement with those of Dakhlaoui [12] and Cardia [16] who found 1,8-Cineole, endo-borneol among the four main compounds in the EO of L. angustifolia. Eldeghedy [15] identified the main constituents of L. angustifolia EO cultivated in Egypt as tau-cadinol (28.63%), eucalyptol (17.21%) and 1-borneol (12.01%) which agreed with Jianu [13] . In the contrast of these results [5,6,[8][9][10][11][12]17] , linalool and linalyl acetate were detected as the major constituents of lavender EO.…”

“…The seeds (to cultivation) of Lavandula angustifolia (Lavender), Lavandula hybrida (lavandin) were imported from Pharmasaat-Seeds and Plants of Medicinal and Spice Herbs Company and propagated in Elmizan Company. The established seedlings were transplanted in the open field of the Experimental Farm, Sekem Company, El-Adlya Belbeis, EL-Sharkiya Governorate, Egypt (30°35'15.65" N and 31°30'7.20" E) according to Eldeghedy [15] , and herbarium specimens were kept in the NRC Herbarium under the Nos. L. angustifolia M138, L. hybrida M139.…”

“…In order to determine and identify the main constituents of each essential oil, samples from each oil were subjected for GC-MS analysis using gas chromatography-mass spectrometry instrument of Medicinal and Aromatic Plants Research Department, National Research Centre following the conditions and the specifications of the instrument and used methods mentioned before by Omer [29] and Eldeghedy [15] .…”

“…essential oils are rich in linalool, camphor, and 1,8-cineole [14] . Eldeghedy [15] reported that the major constituents of L. angustifolia were tau-cadinol, eucalyptol and 1-borneol. While, Cardia [16] reported that the major constituents of Lavandula angustifolia essential oil were 1, 8-cineole (39.83%), borneol (22.63%), and camphor (22.12%).…”

“…While, Kıvrak [10] found the main compound of L. hybrida essential oil was eucalyptol. Eldeghedy [15] reported that the major constituents of L. hybrida were eucalyptol and camphor. Garzoli [14] showed that L. hybrida essential oil had antimicrobial activity against gram-positive (B. cereus and Kocuria marina) and gram-negative bacteria (E. coli, Acinetobacter bohemicus, and P. fluorescens).…”