Essential Oil Composition and Antibacterial Activity of Agrimonia Pilosa Ledeb (Rosaceae)

Abstract

Essential oil composition of the aerial parts of Agrimonia pilosa Ledeb growing wild in the central Himalayan region of Uttarakhand, India was analyzed by capillary gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). A total of 15 constituents were identified, representing 95.29% of the oil composition. The oil consisted mainly of oxygenated monoterpenes (75.74%) followed by monoterpene hydrocarbons (18.68%). Major constituents identified were methyl myrtenate (71.39%), limonene (11.86 %), α-thujone (5.66%) and myrtenyl acetate (3.14%). The essential oil showed a broad spectrum of antibacterial activity against both the human and plant pathogenic bacteria. Oil showed the highest activity against human pathogenic bacteria K. pneumonia (7.33 mm, MIC 175 µL/mL) and P. aeruginosa (7.00 mm, MIC 175 µL/mL). Plant pathogenic bacteria R. solanacearum (6.70 mm, MIC 175 µL/mL) was found less resistant against the oil. The results showed that oil containing methyl myrtenate a major constituent has potential for treatment of infections caused by these pathogenic bacterial strains.

Antibacterial Activity of Four Plant Extracts Extracted from Traditional Chinese Medicinal Plants against Listeria monocytogenes, Escherichia coli, and Salmonella enterica subsp. enterica serovar Enteritidis

“…An accumulating quantity of research has demonstrated that many plants used in traditional Chinese medicine have antibacterial activity both in vitro and in vivo in poultry production [15]. More specifically, the antibacterial properties of solvent extracts of Agrimonia pilosa Ledeb, Smilax glabra Roxb, Anemone chinensis Bunge, and Iris domestica (L.) Goldblatt and Mabb have been documented through in vitro screening [16][17][18][19]. Aqueous extraction methods are inexpensive and the bioactive compounds are less toxic and therefore more suitable for use as a poultry feed supplement.…”