Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration

Cornelison, D.D.W.; Wilcox-Adelman, Sarah A.; Goetinck, Paul F.; Rauvala, Heikki; Rapraeger, Alan C.; Olwin, Bradley B.

doi:10.1101/gad.1214204

Cited by 272 publications

(302 citation statements)

References 20 publications

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“…SDCs have been reported to play crucial role in muscle development, maintenance and regeneration. The role of SDCs has been shown in muscle development in turkey, mice and Drosophila 17, 18, 32, 33, 34. SDC4 is essential during skeletal muscle development and regeneration, and SDC4−/− mice are unable to regenerate damaged muscle 18; however, the underlying mechanisms are poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…The role of SDCs has been shown in muscle development in turkey, mice and Drosophila 17, 18, 32, 33, 34. SDC4 is essential during skeletal muscle development and regeneration, and SDC4−/− mice are unable to regenerate damaged muscle 18; however, the underlying mechanisms are poorly understood. Here we have shown that the expression of SDC4 transiently increased during the early stages of notexin‐induced in vivo regeneration of soleus muscle, in harmony with the earlier observed transient upregulation of SDC4 mRNA 5.…”

Section: Discussionmentioning

confidence: 99%

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Syndecan‐4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition

et al. 2018

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Myostatin, a TGF‐β superfamily member, is a negative regulator of muscle growth. Here we describe how myostatin activity is regulated by syndecan‐4, a ubiquitous transmembrane heparan sulfate proteoglycan. During muscle regeneration the levels of both syndecan‐4 and promyostatin decline gradually after a sharp increase, concurrently with the release of mature myostatin. Promyostatin and syndecan‐4 co‐immunoprecipitate, and the interaction is heparinase‐sensitive. ShRNA‐mediated silencing of syndecan‐4 reduces C2C12 myoblast proliferation via blocking the progression from G1‐ to S‐phase of the cell cycle, which is accompanied by elevated levels of myostatin and p21(Waf1/Cip1), and decreases in cyclin E and cyclin D1 expression. Our results suggest that syndecan‐4 functions as a reservoir for promyostatin regulating the local bioavailability of mature myostatin.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Syndecan‐4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition

et al. 2018

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“…Proteoglycans (PGs) are macromolecules with GAG chains attached to the core. The PGs, especially the heparan sulfate PGs, are important in muscle for maintaining muscle structure, regenerating, growth and development (Cornelison et al, 2004). Inhibition of PG synthesis inhibits the process of skeletal muscle differentiation.…”

Section: Discussionmentioning

confidence: 99%

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

et al. 2013

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Primary muscle cell model systems from farm animals are widely used to acquire knowledge about muscle development, muscle pathologies, overweight issues and tissue regeneration. The morphological properties of a bovine primary muscle cell model system, in addition to cell proliferation and differentiation features, were characterized using immunocytochemistry, western blotting and real-time PCR. We observed a reorganization of the Golgi complex in differentiated cells. The Golgi complex transformed to a highly fragmented network of small stacks of cisternae positioned throughout the myotubes as well as around the nucleus. Different extracellular matrix (ECM) components were used as surface coatings in order to improve cell culture conditions. Our experiments demonstrated improved proliferation and early differentiation for cells grown on surface coatings containing a mixture of both glycosaminoglycans (GAGs) and fibrous proteins. We suggest that GAGs and fibrous proteins mixed together into a composite biomaterial can mimic a natural ECM, and this could improve myogenesis for in vitroin vitro cell cultures.Abbreviations: used: ECMextracellular (The author would like to point out that there should be a space between the abbrevations and the subsequent explanation. ) matrix; GAGsglucosaminoglycans;MRFGAGsglucosaminoglycans, MRF, myogenic regulatory transcription factor; PGproteoglycan

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“…Mouse satellite cells were isolated and cultured as described previously [21]. Briefly, muscle was dissected from the hind limbs of adult female mice (B6D2F1; Jackson labs) between 80 and 130 d of age.…”

Section: Primary Satellite Cell Isolation and Culturementioning

confidence: 99%

“…To detect BrdU incorporation, fixed cells were treated with 4 M hydrochloric acid for 10 min at RT. Primary antibodies used were rat anti-NCAM (Chemicon) at 1:100, chicken anti-syndecan-4 at 1:1500 [21], mouse anti-caveolin-3 (mAb 26, BD Biosciences) at 1:200, mouse anti-BrdU (Roche) at 1:20, and mouse anti-myogenin (F5D; Developmental Studies Hybridoma Bank) neat. For GM1 detection Cholera Toxin Subunit B conjugated to Alexa 488 (Invitrogen) was used at 1:1000.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation

Capkovic

Stevenson

Johnson

et al. 2008

Experimental Cell Research

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Although recent advances in broad-scale gene expression analysis have dramatically increased our knowledge of the repertoire of mRNAs present in multiple cell types, it has become increasingly clear that examination of the expression, localization, and associations of the encoded proteins will be critical for determining their functional significance. In particular, many signaling receptors, transducers, and effectors have been proposed to act in higher-order complexes associated with physically distinct areas of the plasma membrane. Adult muscle stem cells (satellite cells) must, upon injury, respond appropriately to a wide range of extracellular stimuli: the role of such signaling scaffolds is therefore a potentially important area of inquiry. To address this question, we first isolated detergent-resistant membrane fractions from primary satellite cells, then analyzed their component proteins using liquid chromatography-tandem mass spectrometry. Transmembrane and juxtamembrane components of adhesion-mediated signaling pathways made up the largest group of identified proteins; in particular, neural cell adhesion molecule (NCAM), a multifunctional cell-surface protein that has previously been associated with muscle regeneration, was significant. Immunohistochemical analysis revealed that not only is NCAM localized to discrete areas of the plasma membrane, it is also a very early marker of commitment to terminal differentiation. Using flow cytometry, we have sorted physically homogeneous myogenic cultures into proliferating and differentiating fractions based solely upon NCAM expression.

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Essential and separable roles for Syndecan-3 and Syndecan-4 in skeletal muscle development and regeneration

Abstract: Supplemental material is available at http://www.genesdev.org.

Cited by 272 publications

References 20 publications

Syndecan‐4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition

Syndecan‐4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition

The combination of glycosaminoglycans and fibrous proteins improves cell proliferation and early differentiation of bovine primary skeletal muscle cells

Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation

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