“…The optimal dTSAA requirement for achieving the highest EW (63.27 g) and egg mass (59.12 g/bird/d) was estimated to be 589 mg/bird/d and 583 mg/bird/d, respectively, when the average dLYS intake was 652 mg/bird/d ( Figure 1 ). The estimation for dTSAA requirement for optimum egg production performance has been reported ranged from 621 ( Macelline et al, 2021 ) to 565 mg/bird/d ( Macelline et al, 2022 ). The variations in amino acid requirements can be attributed to various experimental conditions, including breed, age, housing system, basal diet, statistical model, and the chosen production criteria ( Akbari Moghaddam Kakhki et al, 2016 ) ( Figure 2 ).…”