Essais de génotoxicité in vitro et in vivo applicables à l'environnement hydrique

Godet, Estelle; Vasseur, B.; Sabut, M.

doi:10.7202/705177ar

Cited by 8 publications

(4 citation statements)

References 43 publications

(13 reference statements)

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“…A number of tests have been developed, using either plants or aquatic animals (see the reviews of Jaylet and Zoll, 1990;Godet et al, 1993), which can potentially serve to assess the genotoxic potency of pollutants in water samples. The assays can be carried out with intact animals, taking into account uptake and elimination, internal transport, and metabolism.…”

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Mouchet

Gauthier

Baudrimont

et al. 2007

Environmental Toxicology

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The toxic and genotoxic potential of Cadmium (CdCl(2)) were evaluated by the micronucleus test (MNT) and comet assay (CA) using amphibian larvae (Xenopus laevis and Pleurodeles waltl). Acute toxicity results showed that Cd is toxic to Xenopus larvae exposed from 2 to 50 mg/L and to Pleurodeles from 5 to 50 mg/L, depending on the nature of the water (reconstituted water containing mineral salts or mineral water MW (Volvic)). The MNT results obtained in MW showed that Cd (2 mg/L) is genotoxic to Xenopus, whereas it was not genotoxic to Pleurodeles at all concentrations tested. The CA established that the genotoxicity of Cd to Xenopus and Pleurodeles larvae depends on the concentration, the exposure times, and the comet parameters (Tail DNA, ETM, OTM, and TL). The CA and MNT results were compared for their ability to detect genotoxic effects, considering the concentrations of Cd applied and the exposure time. The CA showed Cd to be genotoxic from the first day of exposure. In amphibians, the CA appears to be a sensitive and suitable method for detecting genotoxicity such as that caused by Cd.

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Section: Introductionmentioning

confidence: 99%

Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Mouchet

Gauthier

Baudrimont

et al. 2007

Environmental Toxicology

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“…Unrepaired damage can generate other cell lesions and thus lead to tumour formation (Vuillaume 1987;Malins et al 1990). A number of tests have been developed to assess the genotoxic potency of water samples, using either plants or aquatic animals (see the reviews of Jaylet et al 1990;Godet et al 1993). The assays can be carried out with intact animals, taking into account uptake and elimination, internal transport and metabolism.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the potentialin vivoecotoxicity of Double-Walled Carbon Nanotubes (DWNTs) in water, using the amphibianAmbystoma mexicanum

et al. 2007

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Because of their specific properties (mechanical, electrical, etc), carbon nanotubes (CNTs) are being assessed for inclusion in many manufactured products. Due to their massive production and number of potential applications, the impact of CNTs on the environment must be taken into consideration. The present investigation evaluates the ecotoxic potential of CNTs in the amphibian larvae (Ambystoma mexicanum ). Acute toxicity and genotoxicity were analysed after 12 days of exposure in laboratory conditions. The genotoxic effects were analysed by scoring the micronucleated erythrocytes in the circulating blood of the larvae according to the French standard micronucleus assay. The results obtained in the present study demonstrated that CNTs are neither acutely toxic nor genotoxic to larvae whatever the CNTs concentration in the water, although black masses of CNTs were observed inside the gut. In the increasing economical context of CNTs, complementary studies must be undertaken, especially including mechanistic and environmental investigations.

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“…The genotoxicity of unknown matrices is usually evaluated by placing them in contact with organisms which are then examined for genetic damage. A number of tests have been developed, using aquatic animals (see the reviews of Zoll et al, 1990;Godet et al, 1993), which can potentially be used to assess the genotoxic potency of aqueous extracts. The tests can be carried out with intact animals, taking into account uptake and elimination, internal transport and metabolism.…”

Section: Introductionmentioning

confidence: 99%

Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox® and Ames tests)

Mouchet

Gauthier

Mailhes

et al. 2006

Science of The Total Environment

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The management of contaminated soils and wastes is a matter of considerable human concern. The present study evaluates the genotoxic potential of aqueous extracts of two soils (leachates) and of bottom ash resulting from municipal solid waste incineration (MSWIBA percolate), using amphibian larvae (Xenopus laevis). Soil A was contaminated by residues of solvents and metals and Soil B by polycyclic aromatic hydrocarbons and metals. MSWIBA was predominantly contaminated by metals. Two genotoxic endpoints were analysed in circulating erythrocytes taken from larvae: clastogenic and/or aneugenic effects (micronucleus induction) after 12 days of exposure and DNA-strand-breaking potency (comet assay) after 1 and 12 days of exposure. In addition, in vitro bacterial assays (Mutatox and Ames tests) were carried out and the results were compared with those of the amphibian test. Physicochemical analyses were also taken into account. Results obtained with the amphibians established the genotoxicity of the aqueous extracts and the comet assay revealed that they were genotoxic from the first day of exposure. The latter test could thus be considered as a genotoxicity-screening tool. Although genotoxicity persisted after 12 days' exposure, DNA damage decreased overall between days 1 and 12 in the MSWIBA percolate, in contrast to the soil leachates. Bacterial tests detected genotoxicity only for the leachate of soil A (Mutatox). The results confirm the ecotoxicological relevance of the amphibian model and underscore the importance of bioassays, as a complement to physico-chemical data, for risk evaluation.

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Essais de génotoxicité in vitro et in vivo applicables à l'environnement hydrique

Cited by 8 publications

References 43 publications

Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Comparative evaluation of the toxicity and genotoxicity of cadmium in amphibian larvae (Xenopus laevis and Pleurodeles waltl) using the comet assay and the micronucleus test

Assessment of the potentialin vivoecotoxicity of Double-Walled Carbon Nanotubes (DWNTs) in water, using the amphibianAmbystoma mexicanum

Biomonitoring of the genotoxic potential of aqueous extracts of soils and bottom ash resulting from municipal solid waste incineration, using the comet and micronucleus tests on amphibian (Xenopus laevis) larvae and bacterial assays (Mutatox® and Ames tests)

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