Esophageal cancer phospholipids correlated with histopathologic findings: a31P NMR study

Abstract

We analyzed 36 esophageal tumor specimens for phospholipid content using phosphorus nuclear magnetic resonance spectroscopy (31P NMR) and correlated the individual phospholipid profiles with specific clinical and histopathologic features. Among the 18 phospholipids identified in the esophageal tumor specimens, the mean mole percentage concentration of dimethylphosphatidylethanolamine, lysoalkylacylphosphatidylcholine, lysophosphatidic acid, lysophosphatidylcholine (deacylated at the glycerol‐1 carbon), and lysoethanolamine plasmalogen correlated with pathologic T stage, nuclear grade, or the presence of lymphatic invasion. 31P NMR produces well‐dispersed phospholipid spectra and a precise determination of phospholipid relative mole percentages. These data provide a statistical correlation between histopathologic features and molecules known to play an important role in cellular activities and processes unique to malignant tissues. Copyright © 1999 John Wiley & Sons, Ltd.

Nonsurgical Management of Esophageal Cancer

“…By multivariate analysis, Pomp et al found that overexpression of p53 resulted in a decrease in survival in 69 patients with squamous cell carcinoma or adenocarcinoma treated with radiation alone (Pomp et al, 1998). In one study, there was a correlation between decreasing levels of four phospholipids and increasing T stage and grade (Merchant et al, 1999).…”

31P MRS analysis of the phospholipid composition of the peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) of patients with acute leukemia (AL)

“…On the basis of the data from the spectra of PBMC received from patients with AL (AML P and ALL P ), it was claimed that the integral intensities of resonant peaks due to SM and PS and the concentrations of these phospholipids are significantly diminished in comparison with those for healthy volunteers. Similar differences were observed by Merchant et al, who investigated malignant changes in the tissues of the esophagus and colon [11,12].…”

1H‐NMR and 13C‐NMR lipid profiles of human renal tissues

“…A number of these studies regard the lipophilic component of healthy and neoplastic human tissue 1–12. The role of lipids in the life and death of healthy and neoplastic cells is thus well highlighted 13.…”