Esophageal (Barrett's) adenocarcinoma is not associated with Epstein-Barr virus infection: An analysis of 162 cases

Sarbia, Mario; Hausen, Axel zur; Feith, Marcus; Geddert, Helene; Rahden, Burkhard H. A. von; Langer, Rupert; Weyhern, Claus von; Siewert, J R; Höfler, Heinz; Stein, Hubert J.

doi:10.1002/ijc.21190

Cited by 11 publications

(12 citation statements)

References 15 publications

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“…A tissue microarray (TMA), which contained among others 92 carcinomas of the gastric cardia and 89 distal GC [20], was evaluated for cyclin D1 expression. The expression of cyclin D1 as tested by IHC was evaluated by light microscopy.…”

Section: Methodsmentioning

confidence: 99%

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Cyclin D1 expression is induced by viral BARF1 and is overexpressed in EBV-associated gastric cancer

et al. 2008

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Approximately 10% of gastric carcinomas (GC) worldwide are associated with Epstein-Barr virus (EBV). GC is one of the most frequent human malignancies associated with EBV. The latent expression of the EBV-oncogene BARF1 is restricted to epithelial malignancies. To investigate the underlying BARF1-related mechanisms of oncogenic epithelial transformation, we analyzed gene expression profiles of a BARF1-transfected epithelial (HaCaT+) and the corresponding BARF1-negative (HaCaT-) cell line by cDNA microarray analysis. Real-time PCR was performed to confirm the cDNA microarray results. In addition, immunohistochemistry and fluorescence in situ hybridization were performed on a tissue microarray of 181 GC including 11 EBV-associated GC. Among other genes cyclin D1 expression was significantly upregulated in HaCaT+ on the transcriptional and protein level. Cyclin D1 protein expression in GC revealed a significant overexpression of cyclin D1 in EBV-associated GC (p<0.012) but not in EBV-negative GC. Cyclin D1 FISH showed that cyclin D1 overexpression was not due to gene amplification in EBV-associated GC. Cyclin D1 is induced in HaCaT+ by BARF1 and is overexpressed in EBV-associated GC indicating an interaction of viral BARF1 and cyclin D1.

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Section: Methodsmentioning

confidence: 99%

“…The percentage of cyclin D1-positive tumor cells was determined according to the following category: 0 (0%), 1 (1-4%), 2 (5-24%), 3 (25-49%), 4 (50-74%), and 5 (75-100%) [21]. The cyclin D1 expression was correlated to the EBV status which has previously been described [20]. Statistical analyses were performed using Fisher's exact test.…”

Section: Methodsmentioning

confidence: 99%

Cyclin D1 expression is induced by viral BARF1 and is overexpressed in EBV-associated gastric cancer

et al. 2008

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“…From a large ongoing study [23,28], we selected 50 patients with a carcinoma of the gastric cardia and 50 carcinomas, located in the distal stomach, namely in the body and antrum. Cardiac GC were defined as type II and type III carcinomas from the esophago-gastric junction, according to the classification of Siewert and Stein [29].…”

Section: Case Selection and Tissue Microarraysmentioning

confidence: 99%

“…The EBV status of the GC had been tested previously by EBER-RNA in situ hybridization (RISH) on tissue microarrays (TMA) [28]. The TMAs contained three 0.6 mm thick punches per tumor (Beecher Instruments, Silver Spring, MD, USA) and only for signet ring cell carcinomas six instead of three punches were used.…”

Section: Eber-rna In Situ Hybridizationmentioning

confidence: 99%

EBV-infection in cardiac and non-cardiac gastric adenocarcinomas is associated with promoter methylation of p16, p14 and APC, but not hMLH1

et al. 2011

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“…All nasopharyngeal carcinoma biopsies (100%) were positive for EBER1 in almost all carcinoma cells [21]. These studies may give some degree of suspicion for EBV as possible etiological factor contributing to the etiology of EC in Sudan.…”

Section: Discussionmentioning

confidence: 68%

Esophageal Cancer in the Sudan: Focus on the Screening for EBV and the Surgical Management

Ahmed

Beely

Aziz

et al. 2017

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Objective: The aim of this study was to screen for the existence of Epstein Barr Virus (EBV) in esophageal cancer (EC) tissue samples obtained from Sudanese patients and to review the available literature associated with surgical management. Methodology:In this retrospective study 102 formalin fixed paraffin wax embedded tissue's blocks were retrieved from histopathology laboratories in Khartoum City, Sudan and subsequently tested by Immunohistochemical methods for the presence of EBV. All specimens were previously diagnosed as having esophageal carcinoma. Results:As regards Immuno histochemistry (IHC) staining for EBV, positive findings were revealed in 22 (21.5%) and 79/ (78.5%) of the study subjects were negative. Of the 55 males with esophageal cancer, 15/55 (27%) were identified with EBV infection and the remaining 40/55(73%) found without EBV infection. Of the 46 females with esophageal cancer, 7/46(13%) were found positive for EBV immuno staining and the remaining 39/46(87 %) were found negative for EBV. Conclusion:EBV might have some etiological effects contributing to the occurrence of esophageal cancer in Sudan. Crucial preventive strategies are extremely needed to reduce the burden of esophageal cancer in Sudan.

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Esophageal (Barrett's) adenocarcinoma is not associated with Epstein-Barr virus infection: An analysis of 162 cases

Cited by 11 publications

References 15 publications

Cyclin D1 expression is induced by viral BARF1 and is overexpressed in EBV-associated gastric cancer

Cyclin D1 expression is induced by viral BARF1 and is overexpressed in EBV-associated gastric cancer

EBV-infection in cardiac and non-cardiac gastric adenocarcinomas is associated with promoter methylation of p16, p14 and APC, but not hMLH1

Esophageal Cancer in the Sudan: Focus on the Screening for EBV and the Surgical Management

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