Eskape Infection Control: Considerations and Caveats in Combating ESKAPE Pathogens against Nosocomial Infections (Adv. Sci. 1/2020)

Ma, Yu‐Xuan; Wang, Chenyu; Li, Yuanyuan; Li, Jing; Wan, Qian‐qian; Chen, Ji‐Hua; Tay, Franklin R.; L, Niu

doi:10.1002/advs.202070004

Cited by 6 publications

(3 citation statements)

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“…3 These pathogens represent an important dilemma for scientists. 4 On this matter, antimicrobial polymers (AMPs) have gained increasing interest as biocidal agents due to the several advantages compared with conventional antibiotics. Among them, the mechanism of action typically involves the disruption of the cytoplasmic membrane, which severely damages the well-functioning of the microorganism and leads bacterial death.…”

Section: Introductionmentioning

confidence: 99%

Amphiphilic star copolymers composed of curcumin core for bacterial inactivation

Santos,

Fidelis,

Zandonadi

et al. 2023

Polymer International

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Polymeric materials with antimicrobial properties hold the potential to be the next generation of therapeutics. This work reports the preparation of amphiphilic and star‐like copolymers through arm‐first and reversible addition–fragmentation chain transfer polymerization. The copolymers composed of poly(dimethylamine ethyl methacrylate) (PDMAEMA) as the arms and poly(curcumin dimethacrylate) (PCDMA) as the crosslinked core presented a well‐controlled amphiphilic structure with low dispersity values (Ð = 1.15). Varying the [CDMA]:[PDMAEMA] molar ratios (4, 6 and 8) led to a significant increase in the molar mass (153, 688 and 1550 kDa), particle size (33, 90 and 105 nm) and the average number of PDMAEMA arms anchored to the curcumin‐based core (12, 52 and 125 arms), as determined by dynamic and static light scattering. The antimicrobial activity of cationic PDMAEMA40‐co‐PCDMAcore was tested against Escherichia coli in the absence of light and under white light to investigate the photodynamic activity of the copolymers. The results demonstrated a concentration dependence but no additional effect under light irradiation. SEM imaging and live/dead assays revealed extensive membrane damage, evidencing a membrane‐disruption mechanism. HeLa viability assays demonstrated that at 1 mg mL−1, 80 ± 10% of the cells demonstrated metabolic activity after 24 h. © 2023 Society of Industrial Chemistry.

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Section: Introductionmentioning

confidence: 99%

Amphiphilic star copolymers composed of curcumin core for bacterial inactivation

Santos,

Fidelis,

Zandonadi

et al. 2023

Polymer International

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“…Faced with this worrying scenario, great efforts have been made to search for new therapeutic agents that can replace conventional antimicrobials or, at least, reduce the development of antimicrobial resistance (AMR) to them [9,10,11]. Since bacteriophages (viruses that infect bacteria) are species-specific, they represent an interesting alternative for biocontrol.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Phenotypic Characterization of Lytic Bacteriophage with Activity against Pseudomonas aeruginosa

Nascimento

Bertuol²,

Pasqualotto³

et al. 2022

MRJI

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Aims: To perform the isolation and phenotypic characterization of bacteriophage with lytic activity against Pseudomonas aeruginosa. To demonstrate that this type of viral agent can be isolated from the environment and used for the biocontrol of resistant bacterial types, such as Pseudomonas aeruginosa. Study Design: This study was an experimental study. Place and Duration of Study: The study was conducted at, Bacteriology and Mycology Laboratory in the Veterinary Hospital at the School of Agricultural Sciences, Innovation and Business of the University of Passo Fundo (ESAN/UPF) and Center for Diagnosis and Research in Animal Health of the University of Passo Fundo (CDSA/UPF), between April 2022 and June 2022. Methodology: Samples of untreated water were inoculated with the host bacterium strain Pseudomonas aeruginosa ATCC 27853 in an enriched media After the incubation period in, a phage filtrate was obtained by centrifugation followed by filtration. We verified the presence of bacteriophages using spot test and we carried out its purification by the method of sterile toothpick plate transfer on bacterial overlay semi-solid agar. Amplification was performed using an SM buffer elution procedure to produce a stock of viral material. Through assays in Petri dishes with bacterial overlay, we performed titration and phenotypic characterization regarding the lysis spectrum and efficiency of phage infection in the host. Results: We managed to isolate a morphologically characterized lytic bacteriophage with approximately 1 mm of diameter, high clarity in the inhibition area, the presence of halo and well-demarcated edges. The bacteriophage, named as Pseudomonas aeruginosa Phage UPF_PaBP1, demonstrated the infection capacity of the target bacteria in all tested dilutions and a stock preparation with a titre of 6.5 x 10⁸ PFU/ml was obtained for future use. Conclusion: The isolated phage showed strong lytic activity against the bacterial host, a finding that nourishes our expectations regarding the use of this phage as a biocontrol agent and phage therapy.

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“…The ESKAPE pathogens ( Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species ) categorized under a significant group of universally infecting Multi-Drug Resistant pathogens at the heart of the antibiotic resistance calamity 2 . The most prominent virulent members in the group of ESKAPE pathogens are Pseudomonas aeruginosa and methicillin-resistant S. aureus (MRSA) 3 . These pathogens endorse biofilm facilitated infections which cause augmented resistance to antimicrobial drugs, frequently necessitating therapeutic doses several orders of extent greater than those required to eliminate planktonic cells 4 .…”

Section: Introductionmentioning

confidence: 99%

Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

Kannan

Solomon

Krishnamoorthy

et al. 2021

Sci Rep

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In the present study lipopeptide biosurfactant with high emulsification capacity produced by human skin bacterium Paenibacillus thiaminolyticus was purified and subjected to FTIR and NMR spectral analysis which gave evidence of the active characteristics of the surfactant. To augment the antivirulent potential further, the mixer of copper and copper oxide nanoparticles (CuNPs) was synthesized, and characterized by UV–Visible spectroscopy, SEM-EDAX, TEM, and Zeta analysis. Here, we attempted to enhance the antimicrobial and antibiofilm activity with the assistance of encapsulated preparation of lipopeptide and CuNPs in multilamellar liposomes. The proposed mechanism of action of lipopeptide and CuNPs liposomal preparation negatively influences the cell metabolism, secreted virulence such as staphyloxanthin, pyocyanin, and extracellular polysaccharides. The significant decline in the growth of MRSA and P. aeruginosa in both planktonic form and biofilm by lipopeptide and CuNPs treatment were visualized using scanning electron microscopy and High content screening imaging system. In vivo studies revealed that treatment with lipopeptide and CuNPs in multilamellar liposomes extended the lifespan of infected Caenorhabditis elegans by about 75%. Therefore, this study typifies lipopeptide and CuNPs could credibly be a substantial substitute over conventional antibiotics in averting the biofilm associated pathogenesis of MRSA and P. aeruginosa.

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Eskape Infection Control: Considerations and Caveats in Combating ESKAPE Pathogens against Nosocomial Infections (Adv. Sci. 1/2020)

Cited by 6 publications

References 0 publications

Amphiphilic star copolymers composed of curcumin core for bacterial inactivation

Amphiphilic star copolymers composed of curcumin core for bacterial inactivation

Isolation and Phenotypic Characterization of Lytic Bacteriophage with Activity against Pseudomonas aeruginosa

Liposome encapsulated surfactant abetted copper nanoparticles alleviates biofilm mediated virulence in pathogenic Pseudomonas aeruginosa and MRSA

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