ESI-MS/MS Analysis of Phenolic Compounds from Aeonium arboreum Leaf Extracts and Evaluation of their Antioxidant and Antimicrobial Activities

Abstract

Aeonium is a genus of succulents belonging to the Crassulaceae family. Their importance in traditional medicine has stimulated both pharmacological and chemical research. In this study, we optimized extraction, separation, and analytical conditions using a high performance liquid chromatographic method coupled with electrospray ionization mass spectrometry by the negative mode (HPLC-ESI-MS) in order to, for the first time, determine thirty-four compounds from Aeonium arboreum leaves. Twenty-one of them are assigned among which are sixteen flavonoids and five phenolic acids. FRAP, TAC, DPPH, and ABTS•+ radical scavenging were used to evaluate antioxidant activity. The obtained IC50 values ranged from 0.031 to 0.043 mg.mL−1 for DPPH and between 0.048 and 0.09 mg·mL−1 for ABTS•+. Antimicrobial activity was also assessed. The obtained minimum inhibitory concentrations (MIC) of these extracts ranged from 12.5 to 50 µg·mL−1 against Micrococcus luteus, Listeria ivanovii, Staphylococcus aureus, Salmonella enterica, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger, and Fusarium oxysporum, and from 25 to 50 µg·mL−1 against Candida albicans. Therefore, these extracts can be considered as a potential source of biological active compounds.

HESI-MS/MS Analysis of Phenolic Compounds from Calendula aegyptiaca Fruits Extracts and Evaluation of Their Antioxidant Activities

“…Further fragmentation produced the [M-H-162] − ion at m / z 301, which correspond to the loss of glucose. Thus, compound 9 was deduced as Quercetin-3- O -glucoside [ 15 , 16 ]. Peak 11 (T R = 16.96) could be attributed to Quercetin- O -acetyl-glucoside.…”

Antioxidant and Antimicrobial Activities of Erodium arborescens Aerial Part Extracts and Characterization by LC-HESI-MS2 of Its Acetone Extract

“…Total antioxidant capacity was carried out according to Prieto et al [ 66 , 67 ]. An aliquot of 0.1 mL of sample solution containing each extract was combined with 1 mL of reagent solution (0.6 M sulfuric acid, 28 mM sodium phosphate, and 4 mM ammonium molybdate).…”

Effect of Phenolics from Aeonium arboreum on Alpha Glucosidase, Pancreatic Lipase, and Oxidative Stress; a Bio-Guided Approach

“…Fractions (24)(25)(26) were combined and re-chromatographed over SiO 2 CC (50 × 10 cm, 900 mg) using hexane: ethyl acetate at a ratio of (9.5:0.5) as mobile phase to yield 64 sub fractions. Subfractions (17)(18)(19)(20)(21)(22)(23)(24) were pooled together and further purified to yield compound 1 (17.6 mg), whereas fractions (44 and 45) were pooled together, then chromatographed over SiO 2 CC (50 × 7 cm, 770 mg) using dichloromethane and methanol in a ratio (9.5:0.5) as mobile phase to yield 50 subfractions. Subfractions (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) were combined according to their chromatographic pattern to afford compound 2 (21.8 mg).…”

“…According to Hassan et al, limited studies investigated the Aeonium genus biologically and chemically [20]. A few studies were conducted on A. arboreum (L.) Webb & Berthel and proved its antioxidant, antibacterial and antihypertensive properties [21]. Therefore, the purpose of our study was to explore A. arboreum from biological and chemical perspectives.…”