ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT†

Kokkali, Georgia; Coticchio, Giovanni; Bronet, F.; Célébi, Catherine; Cimadomo, Danilo; Goossens, V.; Liss, Joanna; Nunes, Sofia; Sfontouris, Ioannis A.; Vermeulen, Nathalie; Zakharova, E. G.; Rycke, Martine De

doi:10.1093/hropen/hoaa020

Cited by 78 publications

(47 citation statements)

References 14 publications

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“…Recently, the PGT consortium in collaboration with the ESHRE Special Interest Group Embryology issued four consensus papers on Recommendations for good practice in Preimplantation Genetic Testing ( ESHRE PGT Consortium Steering Committee, 2020 ; ESHRE PGT Consortium and SIG-Embryology Biopsy Working Group, 2020 ; ESHRE PGT-M Working Group, 2020 ; ESHRE PGT-SR/PGT-A Working Group, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

ESHRE PGT Consortium data collection XVI–XVIII: cycles from 2013 to 2015†

Coonen

Montfoort

Carvalho

et al. 2020

Human Reproduction Open

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STUDY QUESTION What are the trends and developments in preimplantation genetic testing (PGT) in 2013–2015 as compared to previous years? SUMMARY ANSWER The main trends observed in the retrospective data collections 2013–2015, representing valuable data on PGT activity in (mainly) Europe, are the increased application of trophectoderm biopsy at the cost of cleavage stage biopsy and the continuing expansion of comprehensive testing technology in PGT for chromosomal structural rearrangements and for aneuploidies (PGT-SR and PGT-A). WHAT IS KNOWN ALREADY Since it was established in 1997, the ESHRE PGT Consortium has been collecting data from international PGT centres. To date, 15 data sets and an overview of the first 10 years of data collections have been published. STUDY DESIGN, SIZE, DURATION Collection of (mainly) European data by the PGT Consortium for ESHRE. The data for PGT cycles performed between 1 January 2013 and 31 December 2015 were provided by participating centres on a voluntary basis. For the collection of cycle, pregnancy and baby data, separate, pre-designed MS Excel tables were used. PARTICIPANTS/MATERIALS, SETTING, METHODS Data were submitted by 59, 60 and 59 centres respectively for 2013, 2014 and 2015 (full PGT Consortium members). Records with incomplete or inconsistent data were excluded from the calculations. Corrections, calculations, figures and tables were made by expert co-authors. MAIN RESULTS AND THE ROLE OF CHANCE For data collection XVI/XVII/XVIII, 59/60/59 centres reported data on 8164/9769/11 120 cycles with oocyte retrieval: 5020/6278/7155 cycles for PGT-A, 2026/2243/2661 cycles for PGT for monogenic/single gene defects, 1039/1189/1231 cycles for PGT-SR and 79/59/73 cycles for sexing for X-linked diseases. From 2013 until 2015, the uptake of biopsy at the blastocyst stage was mainly observed in cycles for PGT-A (from 23% to 36%) and PGT-SR (from 22% to 36%), alongside the increased application of comprehensive testing technology (from 66% to 75% in PGT-A and from 36% to 58% in PGT-SR). LIMITATIONS, REASONS FOR CAUTION The findings apply to the 59/60/59 participating centres and may not represent worldwide trends in PGT. Data were collected retrospectively and no details of the follow-up on PGT pregnancies and babies born were provided. WIDER IMPLICATIONS OF THE FINDINGS Being the largest data collection on PGT worldwide, detailed information about ongoing developments in the field is provided. STUDY FUNDING/COMPETING INTEREST(S) The study has no external funding and all costs are covered by ESHRE. There are no competing interests declared. TRIAL REGISTRATION NUMBER N/A.

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Section: Introductionmentioning

confidence: 99%

ESHRE PGT Consortium data collection XVI–XVIII: cycles from 2013 to 2015†

Coonen

Montfoort

Carvalho

et al. 2020

Human Reproduction Open

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“…This developmentally regulated adhesion process requires the calcium-dependent cell-cell adhesion molecule CDH1 (formerly known as E-cadherin or uvomorulin) [90,[93][94][95]. Removing extracellular calcium prevents CDH1 binding and causes embryos to de-compact [96][97][98]. The compaction process was long thought to be driven by increased adhesion of cells via modifications of the CDH1dependent adhesion machinery [1,99].…”

Section: Compactionmentioning

confidence: 99%

Morphogenesis of the human preimplantation embryo: bringing mechanics to the clinics

Firmin

Maître

2021

Seminars in Cell & Developmental Biology

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During preimplantation development, the human embryo forms the blastocyst, the structure enabling uterine implantation. The blastocyst consists of an epithelial envelope, the trophectoderm, encompassing a fluid-filled lumen, the blastocoel, and a cluster of pluripotent stem cells, the inner cell mass. This specific architecture is crucial for the implantation and further development of the human embryo. Furthermore, the morphology of the human embryo is a prime determinant for clinicians to assess the implantation potential of in vitro fertilized human embryos, which constitutes a key aspect of assisted reproduction technology.Therefore, it is crucial to understand how the human embryo builds the blastocyst. As any material, the human embryo changes shape under the action of forces. Here, we review recent advances in our understanding of the mechanical forces shaping the blastocyst. We discuss the cellular processes responsible for generating morphogenetic forces that were studied mostly in the mouse and review the literature on human embryos to see which of them may be conserved. Based on the specific morphological defects commonly observed in clinics during human preimplantation development, we discuss how mechanical forces and their underlying cellular processes may be affected. Together, we propose that bringing tissue mechanics to the clinics will advance our understanding of human preimplantation development, as well as our ability to help infertile couples to have babies.

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“…Intracytoplasmic sperm injection (ICSI) or the injection of a single spermatozoon directly into the ooplasma of the oocyte represented the major breakthrough for male infertility treatment (Palermo et al 1992, Tarlatzis & Bili 2000. This fertilisation technique is specifically recommended for PGT treatment, in order to avoid paternal contamination from sperm attached to the zona pellucida as in conventional IVF (Kokkali et al 2020). Maternal DNA contamination originating from cumulus cells can as well be reduced by careful denudation of the oocytes prior to ICSI (Wilton et al 2009).…”

Section: Icsi Embryo Biopsy Transfer and Cryopreservationmentioning

confidence: 99%

PREIMPLANTATION GENETIC TESTING: Clinical experience of preimplantation genetic testing

et al. 2020

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Thirty years of rapid technological advances in the field of genetic testing and assisted reproduction have reshaped the procedure of preimplantation genetic testing (PGT). The development of whole genome amplification and genome-wide testing tools together with the implementation of optimal hormonal stimulation protocols and more efficient cryopreservation methods have led to more accurate diagnoses and improved clinical outcomes. In addition, the shift towards embryo biopsy at day 5/6 has changed the timeline of a typical PGT clinical procedure. The Brussels' PGT centre started more than 25 years ago, offering PGT within a healthcare system funded by the government. In this paper, we have summarized our experience with PGT, presenting an up-to-date overview of the different steps in PGT from patient referral to baby follow-up.

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ESHRE PGT Consortium and SIG Embryology good practice recommendations for polar body and embryo biopsy for PGT†

Cited by 78 publications

References 14 publications

ESHRE PGT Consortium data collection XVI–XVIII: cycles from 2013 to 2015†

ESHRE PGT Consortium data collection XVI–XVIII: cycles from 2013 to 2015†

Morphogenesis of the human preimplantation embryo: bringing mechanics to the clinics

PREIMPLANTATION GENETIC TESTING: Clinical experience of preimplantation genetic testing

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