2009
DOI: 10.1042/bst0370178
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ESCRT proteins and the regulation of endocytic delivery to lysosomes

Abstract: In mammalian cells, there is evidence of cargo specificity in the requirement for particular ESCRT (endosomal sorting complex required for transport) proteins to sort cargo into the luminal vesicles of MVBs (multivesicular bodies). We have focussed on studying the ESCRT requirements for delivery of MHC class I to lysosomes following polyubiquitination by the Kaposi's sarcoma-associated herpesvirus protein K3. Down-regulation of polyubiquitinated cell-surface MHC class I in HeLa cells stably expressing K3 is ac… Show more

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Cited by 16 publications
(12 citation statements)
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“…ESCRT complexes play a central role in the entire process. ESCRT, which interacts with ubiquitinated proteins (Luzio et al, 2009), takes cargo proteins to the late endosomes and assembles them into exosomes. Besides ESCRT, various signaling proteins including Rab small GTP binding proteins and their signaling partners (GEFs and GAPs) (Liu and Storrie, 2012), are deeply involved not only in the formation of exosomes, but also in the release of exosomes to the extracellular space.…”
Section: Cell-cell Communication Via Exosomesmentioning
confidence: 99%
See 1 more Smart Citation
“…ESCRT complexes play a central role in the entire process. ESCRT, which interacts with ubiquitinated proteins (Luzio et al, 2009), takes cargo proteins to the late endosomes and assembles them into exosomes. Besides ESCRT, various signaling proteins including Rab small GTP binding proteins and their signaling partners (GEFs and GAPs) (Liu and Storrie, 2012), are deeply involved not only in the formation of exosomes, but also in the release of exosomes to the extracellular space.…”
Section: Cell-cell Communication Via Exosomesmentioning
confidence: 99%
“…(Clayton et al, 2007;Turola et al, 2012). Of interest, a large portion of proteins in exosomes are found polyubiquitinated (Luzio et al, 2009), suggesting that exosomal proteins are subjected to protein degradation by lysosomal enzymes unless they are released to the extracellular space following fusion of the late endosomes to the plasma membrane Exosomes as a carrier of RNAs Besides proteins, exosomes also carry nucleic acids (Chaput and Thery, 2011;Zomer et al, 2010). An earlier study showed that exosomes isolated from the blood of patients chronically infected with hepatitis C virus (HCV) carried HCV-specific RNA species.…”
Section: Molecular Composition Of Exosomesmentioning
confidence: 99%
“…Finally, the activity of an AAAATPase (Vps4) leads to dissociation of the ESCRT complexes. Delivery of the ILVs to the lysosome interior then occurs by fusion of the LE with the lysosome (Luzio et al, 2009).…”
Section: Introductionmentioning
confidence: 99%
“…Initially, Rab5 is recruited to the early endosome and is exchanged with Rab7 on the intermediate phagosome [94,[97][98][99][100][101]. From here, Rab7 is required for the recruitment of Rab9, which completes the fusion of the maturating phagosome with the lysosome [94,[98][99][100][101]. At this point, the mature phagolysosome has Cathepsin D and vATPase markers and is actively acidifying to pH 4.0 in order to degrade the vacuole contents [95].…”
Section: Subversion Of the Phagolysosome By Other Pathogensmentioning
confidence: 99%
“…Rab5, Rab7, and Rab9 are key contributors to the phagosome maturation pathway [94][95][96][97][98][99][100][101]. Initially, Rab5 is recruited to the early endosome and is exchanged with Rab7 on the intermediate phagosome [94,[97][98][99][100][101].…”
Section: Subversion Of the Phagolysosome By Other Pathogensmentioning
confidence: 99%