ESCRT‐dependent vacuolar sorting and degradation of the auxin biosynthetic enzyme YUC1 flavin monooxygenase

Ge, Chennan; Gao, Caiji; Chen, Qingguo; Jiang, Liwen; Zhao, Yunde

doi:10.1111/jipb.12760

Cited by 10 publications

(8 citation statements)

References 13 publications

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“…The major and canonical function of ESCRT is to capture and sort ubiquitinated membrane proteins into the intraluminal vesicles (ILVs) within MVBs, which then fuse with the vacuole and release the ILV-containing membrane cargo into the vacuolar lumen for degradation (Spitzer et al 2009;Buono et al 2016;Gao et al 2017;Vietri et al 2019). The ESCRT machinery in plants regulates the sorting and vacuolar trafficking of some plant-specific soluble cargos, such as the abscisic acid (ABA) receptors PYR/PYL/RCAR and the auxin biosynthesis enzyme YUC1 flavin monooxygenase (Belda-Palazon et al 2016;Yu et al 2016;Yu and Xie 2017;Ge et al 2019;Chen et al 2020).…”

Section: Introductionmentioning

confidence: 99%

SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants

Xiao

Yang

Liu

et al. 2020

JIPB

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The endosomal sorting complex required for transport (ESCRT) machinery is an ancient, evolutionarily conserved membrane remodeling complex that is essential for multivesicular body (MVB) biogenesis in eukaryotes. FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant‐specific ESCRT component, modulates MVB‐mediated endosomal sorting and autophagic degradation. Although the basic cellular functions of FREE1 as an ESCRT component have been described, the regulators that control FREE1 turnover remain unknown. Here, we analyzed how FREE1 homeostasis is mediated by the RING‐finger E3 ubiquitin ligases, SINA of Arabidopsis thaliana (SINATs), in response to iron deficiency. Under iron‐deficient growth conditions, SINAT1‐4 were induced and ubiquitinated FREE1, thereby promoting its degradation and relieving the repressive effect of FREE1 on iron absorption. By contrast, SINAT5, another SINAT member that lacks ubiquitin ligase activity due to the absence of the RING domain, functions as a protector protein which stabilizes FREE1. Collectively, our findings uncover a hitherto unknown mechanism of homeostatic regulation of FREE1, and demonstrate a unique regulatory SINAT–FREE1 module that subtly regulates plant response to iron deficiency stress.

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Section: Introductionmentioning

confidence: 99%

SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants

Xiao

Yang

Liu

et al. 2020

JIPB

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“…The ESCRT machinery is required for sorting the ubiquitinated cargo proteins into invaginations of endosome membranes, and eventually degradation in the lysosome/vacuole (Raiborg and Stenmark, 2009; Otegui, 2018). It was also demonstrated that YUC1 was sorted to vacuoles for degradation through the ESCRT pathway (Ge et al ., 2018). In this study, we show that VPS28A and VPS28B play essential roles in endosomal trafficking of PINs.…”

Section: Discussionmentioning

confidence: 99%

“…AtBRO1 also interacts with the ESCRT‐III component SNF7, and recruits the DUb enzyme to late endosomes to remove ubiquitin from ubiquitinated cargoes before internalization of the cargoes into ILVs (Cardona‐Lopez et al ., 2015; Kalinowska et al ., 2015). It was shown that the auxin‐biosynthetic enzyme YUC1 flavin monooxygenase, a soluble protein, is sorted to vacuoles through the ESCRT pathway (Ge et al ., 2018). These studies indicated that ESCRT machinery plays important roles in plant growth and development (Gao et al ., 2017).…”

Section: Introductionmentioning

confidence: 99%

The ESCRT‐I components VPS28A and VPS28B are essential for auxin‐mediated plant development

2020

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The highly conserved endosomal sorting complex required for transport (ESCRT) pathway plays critical roles in endosomal sorting of ubiquitinated plasma membrane proteins for degradation. However, the functions of many components of the ESCRT machinery in plants remain unsolved. Here we show that the ESCRT-I subunits VPS28A and VPS28B are functionally redundant and required for embryonic development in Arabidopsis. We conducted a screen for genetic enhancers of pid, which is defective in auxin signaling and transport. We isolated a no-cotyledon in pid 104 (ncp104) mutant, which failed to develop cotyledons in a pid background. We discovered that ncp104 was a unique recessive gain-of-function allele of vps28a. VPS28A and VPS28B were expressed during embryogenesis and the proteins were localized to the trans-Golgi network/early endosome and post-Golgi/endosomal compartments, consistent with their functions in endosomal sorting and embryogenesis. The single vps28a and vps28b loss-of-function mutants did not display obvious developmental defects, but their double mutants showed abnormal cell division patterns and were arrested at the globular embryo stage. The vps28a vps28b double mutants showed altered auxin responses, disrupted PIN1-GFP expression patterns, and abnormal PIN1-GFP accumulation in small aberrant vacuoles. The ncp104 mutation may cause the VPS28A protein to become unstable and/or toxic. Taken together, our findings demonstrate that the ESCRT-I components VPS28A and VPS28B redundantly play essential roles in vacuole formation, endosomal sorting of plasma membrane proteins, and auxin-mediated plant development.

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“…Besides autophagy, the endosomal sorting complex required for transport (ESCRT) complex also regulates protein sorting to the vacuole, which is significant to multivesicular body (MVB) biogenesis and MVB-mediated vacuolar transport pathway [9,10]. Cell membrane proteins like the receptors and transporters are internalized into vesicles and delivered to the trans-Golgi network (TGN), which is defined as the early endosome in plants [11,12]. Depending on the demand, some TGN-localized proteins are recycled back to the cell membrane, while others are further transported to the late endosome, MVB, where those degraded membrane proteins are sorted to the intraluminal vesicles (ILVs) inside the MVB.…”

Section: Introductionmentioning

confidence: 99%

“…Depending on the demand, some TGN-localized proteins are recycled back to the cell membrane, while others are further transported to the late endosome, MVB, where those degraded membrane proteins are sorted to the intraluminal vesicles (ILVs) inside the MVB. Finally, the ILVs are released to the vacuolar lumen for degradation upon the fusion between the MVB and the vacuole [12,13]. During this process, the ESCRT complex plays essential roles in the sorting of ubiquitinated membrane proteins and the deformation of the MVB membrane to form ILVs [14,15].…”

Section: Introductionmentioning

confidence: 99%

Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency

Zhang

Xiao

Liu

et al. 2021

IJMS

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Multivesicular body (MVB)-mediated endosomal sorting and macroautophagy are the main pathways mediating the transport of cellular components to the vacuole and are essential for maintaining cellular homeostasis. The interplay of these two pathways remains poorly understood in plants. In this study, we show that FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT), essential for MVB biogenesis and plant growth, can be transported to the vacuole for degradation in response to iron deficiency. The vacuolar transport of ubiquitinated FREE1 protein is mediated by the autophagy pathway. As a consequence, the autophagy deficient mutants, atg5-1 and atg7-2, accumulate more endogenous FREE1 protein and display hypersensitivity to iron deficiency. Furthermore, under iron-deficient growth condition autophagy related genes are upregulated to promote the autophagic degradation of FREE1, thereby possibly relieving the repressive effect of FREE1 on iron absorption. Collectively, our findings demonstrate a unique regulatory mode of protein turnover of the ESCRT machinery through the autophagy pathway to respond to iron deficiency in plants.

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ESCRT‐dependent vacuolar sorting and degradation of the auxin biosynthetic enzyme YUC1 flavin monooxygenase

Cited by 10 publications

References 13 publications

SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants

SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants

The ESCRT‐I components VPS28A and VPS28B are essential for auxin‐mediated plant development

Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency

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