Escherichia coli with extended-spectrum beta-lactamases or transferable AmpC beta-lactamases and Salmonella on meat imported into Sweden

Egervärn, Maria; Börjesson, Stefan; Byfors, Sara; Finn, Maria; Kaipe, Caroline; Englund, Stina; Lindblad, M.

doi:10.1016/j.ijfoodmicro.2013.11.005

Cited by 82 publications

(78 citation statements)

References 29 publications

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“…The inability to persist on animal level might be due to an unsuccessful combination of CMY-2-E. coli on plasmid IncA/C. In European broiler meat, CMY-2-E. coli is often found in combination with plasmids IncI1 or IncK (Borjesson et al, 2013;Egervarn et al, 2014). The low occurrence and the observed decrease in this study may suggest that plasmid IncA/C is less able to conjugate and spread in bacterial populations as was previously described for Salmonella (Poole and Crippen, 2009).…”

Section: Resultssupporting

confidence: 54%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

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A B S T R A C TExtended-spectrum β-lactamase and plasmid mediated AmpC β-lactamase (ESBL/pAmpC) producing bacteria are resistant to Extended Spectrum Cephalosporins (ESC), and are present in all levels of the broiler production chain. We determined the prevalence, concentration, and persistence of ESBL/pAmpC-Escherichia coli in a broiler parent flock during the rearing and laying period. One-day old chickens were housed in four separate pens. Until week 33 no antibiotics or coccidiostatics were used. During rearing 57 chickens in each pen (n = 228), and in the laying period two groups of 33 chickens were individually sampled (n = 66). Environmental samples were taken from week 16 onwards. ESBL/pAmpC-E. coli presence was determined by selective culturing. In the samples of week 16-19 the concentration of ESBL/pAmpC-E. coli was determined. All ESC-resistant isolates found were positive for pAmpC gene bla CMY-2 located on IncA/C plasmids, in several E. coli MLST types. CMY-2-E. coli prevalence decreased from 91% (95%CI 86-94%) at day 7 (week 1) to 0% (95%CI 0-5%) in week 21. However, CMY-2-E. coli remained present in the environmental samples during the whole study. CMY-2-E. coli concentration varied between detection limit (< 10^3) and 2·10^4 cfu/g faeces. The sharp reduction of CMY-2-E. coli in this broiler parent flock in absence of antibiotics suggests a selective disadvantage of bla CMY-2 on IncA/C plasmids on animal level. The underlying mechanism should be studied further as this may provide new insights on how to reduce ESBL/pAmpC prevalence and transmission in the broiler production chain.

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Section: Resultssupporting

confidence: 54%

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dame-Korevaar

Fischer

Stegeman

et al. 2017

Veterinary Microbiology

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“…The investigation performed in Sweden on meat imported from European countries indicated resistance to ciprofloxacin in more than 50% of E. coli carrying ESBL genes isolated from broiler meat (6), as well as in three out of seven strains recovered from beef and in one out of seven isolates of pork origin. Few of the E. coli strains carrying ESBL genes found in the Swedish investigation showed resistance to gentamycin (one isolated from poultry meat and one from beef) (6). In the present study, none of the ESBL-producing E. coli isolates was resistant to gentamycin.…”

Section: Discussioncontrasting

confidence: 53%

“…Recently, reports confirming the presence of these strains in meat and their antimicrobial resistance have been published (2,(6)(7)(8). The study conducted in Turkey (8) showed that all 20 ESBL-producing E. coli isolated from meat were susceptible to cefepime.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance of ESBLand AmpC-producing Escherichia coli isolated from meat

Wasiński

Różańska

Osek

2014

Bulletin of the Veterinary Institute in Pulawy

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In the present study, 25 Escherichia coli strains isolated from beef, pork, and poultry meat, and producing extendedspectrum β-lactamases (ESBL) (18 strains) or AmpC-cephalosporinases (7 strains) were tested for antimicrobial resistance using the minimum inhibitory concentration method with 16 antimicrobial agents. All examined strains were resistant to ampicillin and the first-generation cephalosporins. Variable resistance to the third-generation cephalosporins (40%-100% among ESBLproducing strains and 0-72% among AmpC-producing strains) was noted. Less than 30% of examined strains were resistant to ciprofloxacin. All isolates were susceptible to the fourth-generation cephalosporins, cephalosporins connected with inhibitors of β-lactamases, carbapenems, and gentamycin.

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“…Paratyphi B was the most prevalent in chicken meat, consistently with the report of Boscán et al (2005), who isolated the bacteria from chicken viscera in two slaughter plants in the state of Zulia, Venezuela, and the report by Barua et al (2013), who isolated this serotype from breeders and broiler farms in Bangladesh. S. Paratyphi B is known to be adapted to commercial poultry (Toboldt et al, 2013;van de Giessen et al, 2006;Van Immerseel et al, 2004), and it can be isolated from farm to store (Egervärn et al, 2014). S. Muenster has been isolated from pork sausages (Torres et al, 2013), ground beef (Bosilevacet al, 2009), cheese from goat milk (Van Cauteren et al, 2009), pork and poultry (Meneses, 2010).…”

Section: Resultsmentioning

confidence: 99%

Serotypes of Salmonella in Broiler Carcasses Marketed at Ibague, Colombia.

Rodriguez

Rondón

Verján

2015

Rev. Bras. Cienc. Avic.

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Salmonella enterica is a large group of Gram-negative bacteria responsible for a number of foodborne infections associated with the consumption of contaminated poultry products. The hygienic status of raw chicken meat marketed at Ibague, Tolima, Colombia, is currently unknown. To address this issue, a cross-sectional study was conducted to estimate the prevalence of Salmonella spp., in raw chicken marketed at different outlets in this city. Salmonella spp. was isolated by standard microbiological methods, followed by biochemical, serological, and molecular confirmation. Additionally, risk factors associated with the presence of the bacteria were identified. The prevalence of Salmonella in raw chicken was 17.41% (47/270), and 14 different serotypes were found, out of which S. Paratyphi B (36.17%), S. Hvittingfoss (19.15%) and S. Muenster (10.64%) were the most prevalent and represented 65.95% of all serotypes. Amplification of 284 bp of the invA gene was achieved by PCR in a number of randomly selected isolates. Raw chicken as the only type of meat sold at stores (odds ratio: 2,157, p<0.05), and stainless steel as a contact surface of chicken meat (odds ratio: 13,29, p<0.05), were found to be potential risk factors for the presence of Salmonella in chicken meat. This work serves as a reference about the current status of Salmonella in chicken meat marketed in Ibague, Tolima, Colombia, and indicates the need to establish appropriate control and contingency measures to minimize the presence of the bacteria in raw chicken to prevent its transmission to humans.

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Escherichia coli with extended-spectrum beta-lactamases or transferable AmpC beta-lactamases and Salmonella on meat imported into Sweden

Cited by 82 publications

References 29 publications

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Dynamics of CMY-2 producing E. coli in a broiler parent flock

Antimicrobial resistance of ESBLand AmpC-producing Escherichia coli isolated from meat

Serotypes of Salmonella in Broiler Carcasses Marketed at Ibague, Colombia.

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