Escherichia coli Strains Isolated from Retail Meat Products: Evaluation of Biofilm Formation Ability, Antibiotic Resistance, and Phylogenetic Group Analysis

Barilli, Elena; Vismarra, Alice; Frascolla, Viviana; Rega, Martina; Bacci, C.

doi:10.4315/0362-028x.jfp-19-330

Cited by 10 publications

(8 citation statements)

References 35 publications

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“…This observation showed that on the one hand, multidrug resistance of these strains may enhance their virulence, especially in broiler isolates, and on the other hand, the general capacity of the mcr-1- positive E. coli strains to produce biofilms was at the medium and low level (no strong biofilm producers were observed in this study). Similar results were obtained by Barilli et al [ 68 ], wherein E. coli strains isolated from retail meat products (including poultry) were weak biofilm producers. Although the tested in vitro strains did not show a strong biofilm production, it is worth noting that under appropriate in vivo conditions, with insufficient production hygiene, biofilm production may be more effective.…”

Section: Discussionsupporting

confidence: 89%

Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

et al. 2021

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Background A plasmid-mediated mechanism of bacterial resistance to polymyxin is a serious threat to public health worldwide. The present study aimed to determine the occurrence of plasmid-mediated colistin resistance genes and to conduct the molecular characterization of mcr-positive Escherichia coli strains isolated from Polish poultry. Methods In this study, 318 E. coli strains were characterized by the prevalence of mcr1–mcr5 genes, antimicrobial susceptibility testing by minimal inhibitory concentration method, the presence of antimicrobial resistance genes was screened by PCR, and the biofilm formation ability was tested using the crystal violet staining method. Genetic relatedness of mcr-1-positive E. coli strains was evaluated by multilocus sequence typing method. Results Among the 318 E. coli isolates, 17 (5.35%) harbored the mcr-1 gene. High antimicrobial resistance rates were observed for ampicillin (100%), tetracycline (88.24%), and chloramphenicol (82.35%). All mcr-1-positive E. coli strains were multidrug-resistant, and as many as 88.24% of the isolates contained the blaTEM gene, tetracycline (tetA and tetB), and sulfonamide (sul1, sul2, and sul3) resistance genes. Additionally, 41.18% of multidrug-resistant, mcr-1-positive E. coli isolates were moderate biofilm producers, while the rest of the strains showed weak biofilm production. Nine different sequence types were identified, and the dominant ST was ST93 (29.41%), followed by ST117 (17.65%), ST156 (11.76%), ST 8979 (11.76%), ST744 (5.88%), and ST10 (5.88%). Moreover, the new ST was identified in this study. Conclusions Our results showed a low occurrence of mcr-1-positive E. coli strains isolated from Polish poultry; however, all the isolated strains were resistant to multiple antimicrobial agents and were able to form biofilms at low or medium level.

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Section: Discussionsupporting

confidence: 89%

Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

et al. 2021

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“…The rate of biofilm production has been reported as being high (60%) in bacteria that has been isolated from other wild animals, even if the available data frequently depend on the strain origin, cell membrane, surfaces, culture medium and methodology that were used [ 54 ]. Thanks to the biofilm matrix, biofilm-producing bacteria can tolerate harsh living conditions in the environment [ 55 ], and at the same time, this situation can promote the exchange of genetic elements, thus facilitating the spread of antimicrobial resistance through the bacteria [ 56 ]. In fact, different studies have demonstrated that the majority of bacteria which are able to produce biofilm are MDR [ 54 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

“…Thanks to the biofilm matrix, biofilm-producing bacteria can tolerate harsh living conditions in the environment [ 55 ], and at the same time, this situation can promote the exchange of genetic elements, thus facilitating the spread of antimicrobial resistance through the bacteria [ 56 ]. In fact, different studies have demonstrated that the majority of bacteria which are able to produce biofilm are MDR [ 54 , 56 ]. The data that were generated in this study showed the high ability of biofilm formation in resistant bacteria, both in pork and wildboar meat.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistant E. coli in Pork and Wild Boar Meat: A Risk to Consumers

Rega

Andriani

Cavallo

et al. 2022

Foods

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Antimicrobial-resistant foodborne microorganisms may be transmitted from food producing animals to humans through the consumption of meat products. In this study, meat that was derived from farmed pigs and wild boars was analyzed and compared. Escherichia coli (E. coli) were isolated and tested phenotypically and genotypically for their resistance to quinolones, aminoglycosides and carbapenems. The co-presence of AMR-associated plasmid genes was also evaluated. A quinolone AMR phenotypic analysis showed 41.9% and 36.1% of resistant E. coli derived from pork and wild boars meat, respectively. A resistance to aminoglycosides was detected in the 6.6% of E. coli that was isolated from pork and in 1.8% of the wild boar meat isolates. No resistant profiles were detected for the carbapenems. The quinolone resistance genes were found in 58.3% of the phenotypically resistant pork E. coli and in 17.5% of the wild boar, thus showing low genotypic confirmation rates. The co-presence of the plasmid-related genes was observed only for the quinolones and aminoglycosides, but not for the carbapenems. Wild boar E. coli were the most capable to perform biofilm production when they were compared to pork E. coli. In conclusion, the contamination of pork and wild boar meat by AMR microorganisms could be a threat for consumers, especially if biofilm-producing strains colonize the surfaces and equipment that are used in the food industry.

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“…The Italian situation, in particular in Emilia Romagna and Lombardia (North Italy) showed a prevalence of ESβL E. coli isolated from pork fresh meat and meat products of 9.2% and 5.0%, respectively [ 33 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar

et al. 2021

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A global increase in Escherichia coli (E. coli) resistant to cephalosporins (extended-spectrum β-lactamases (ESβLs) and AmpC β-lactamases) has been recorded in the last 20 years. Similarly, several studies have reported the spread of colistin resistance in Enterobacteriaceae isolated from food and the environment. The aim of the present study was to evaluate the prevalence of ESβL, AmpC and colistin-resistant E. coli isolated from pork and wild boar meat products in the Emilia Romagna region (North Italy). The isolates were analysed phenotypically (considering both resistant and intermediate profiles) and genotypically. The prevalence of genotypically confirmed ESβL and AmpC E. coli was higher in pork meat products (ESβL = 11.1% vs. AmpC = 0.3%) compared to wild boar meat (ESβL = 6.5% vs. AmpC = 0%). Intermediate profiles for cefotaxime (CTX) and ceftazidime (CAZ) were genotypically confirmed as ESβL in pork meat isolates but not for wild boar. Four E. coli from wild boar meat were resistant to colistin but did not harbour the mcr-1 gene. E. coli isolated from wild boar meat seem to show aspecific antimicrobial resistance mechanisms for cephalosporins and colistin. The prevalence of resistant isolates found in wild boar is less alarming than in pork from farmed domestic pigs. However, the potential risk to consumers of these meat products will require further investigations.

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Escherichia coli Strains Isolated from Retail Meat Products: Evaluation of Biofilm Formation Ability, Antibiotic Resistance, and Phylogenetic Group Analysis

Cited by 10 publications

References 35 publications

Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

Phenotypic and genotypic characterization of mcr-1-positive multidrug-resistant Escherichia coli ST93, ST117, ST156, ST10, and ST744 isolated from poultry in Poland

Antimicrobial Resistant E. coli in Pork and Wild Boar Meat: A Risk to Consumers

Prevalence of ESβL, AmpC and Colistin-Resistant E. coli in Meat: A Comparison between Pork and Wild Boar

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