Escherichia coli O8:H8 Carrying a Novel Variant of the Heat-Labile Enterotoxin LT2 Gene Caused Outbreaks of Diarrhea

Ishimaru, Kai; Sasaki, Mari; Narimatsu, Hiroshi; Arimizu, Yoko; Gotoh, Yasuhiro; Nakamura, Keiji; Hayashi, Tetsuya

doi:10.1093/ofid/ofaa021

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…Given that, according to a previous study, some Stx2k-STEC strains carried heat-stable and heat-labile enterotoxin-encoding genes ( est and elt ) ( 12 ), we further subtyped est and elt genes by comparing the assemblies against an in-house database, including representative nucleotide sequences of est and elt , using ABRicate version 0.8.10. Nucleotide sequences of different est and elt subtypes were obtained from previous reports ( 46 – 48 ).…”

Section: Methodsmentioning

confidence: 99%

High Prevalence and Persistence of Escherichia coli Strains Producing Shiga Toxin Subtype 2k in Goat Herds

et al. 2022

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Section: Methodsmentioning

confidence: 99%

High Prevalence and Persistence of Escherichia coli Strains Producing Shiga Toxin Subtype 2k in Goat Herds

et al. 2022

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“…The enterotoxin-encoding genes were extracted and compared against an in-house database including representative nucleotide sequences of the heat-stable and heat-labile enterotoxin-encoding gene st and lt, using ABRicate version 0.8.10. Nucleotide sequences of different st and lt subtypes were obtained from previous reports [57][58][59]. Multilocus sequence typing (MLST) of seven housekeeping genes was performed through an on-line tool provided by the Warwick E. coli MLST scheme website (https://enterobase.…”

Section: Determination Of Stx 2 Subtype Serotype Mlst Virulence Facto...mentioning

confidence: 99%

Genomic Characteristics of Stx2e-Producing Escherichia coli Strains Derived from Humans, Animals, and Meats

Yang

Wu²,

Liu

et al. 2021

Pathogens

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Shiga toxin (Stx) can be classified into two types, Stx1 and Stx2, and different subtypes. Stx2e is a subtype commonly causing porcine edema disease and rarely reported in humans. The purpose of this study was to analyze the prevalence and genetic characteristics of Stx2e-producing Escherichia coli (Stx2e-STEC) strains from humans compared to strains from animals and meats in China. Stx2e-STEC strains were screened from our STEC collection, and whole-genome sequencing was performed to characterize their genetic features. Our study showed a wide distribution of Stx2e-STEC among diverse hosts and a higher proportion of Stx2e-STEC among human STEC strains in China. Three human Stx2e-STEC isolates belonged to O100:H30, Onovel26:H30, and O8:H9 serotypes and varied in genetic features. Human Stx2e-STECs phylogenetically clustered with animal- and food-derived strains. Stx2e-STEC strains from animals and meat showed multidrug resistance, while human strains were only resistant to azithromycin and tetracycline. Of note, a high proportion (55.9%) of Stx2e-STEC strains, including one human strain, carried the heat-stable and heat-labile enterotoxin-encoding genes st and lt, exhibiting a STEC/enterotoxigenic E. coli (ETEC) hybrid pathotype. Given that no distinct genetic feature was found in Stx2e-STEC strains from different sources, animal- and food-derived strains may pose the risk of causing human disease.

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“…Flavodoxin in human gastrointestinal pathogens a . The information related to the bacterial genera which cause gastrointestinal diseases was extracted from References[171][172][173][174][175][176][177][178][179][180][181][182][183]. Unreviewed indicates flavodoxin sequences reported as such in Uniprot.…”

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confidence: 99%

Flavodoxins as Novel Therapeutic Targets against Helicobacter pylori and Other Gastric Pathogens

Salillas

Sancho

2020

IJMS

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Flavodoxins are small soluble electron transfer proteins widely present in bacteria and absent in vertebrates. Flavodoxins participate in different metabolic pathways and, in some bacteria, they have been shown to be essential proteins representing promising therapeutic targets to fight bacterial infections. Using purified flavodoxin and chemical libraries, leads can be identified that block flavodoxin function and act as bactericidal molecules, as it has been demonstrated for Helicobacter pylori (Hp), the most prevalent human gastric pathogen. Increasing antimicrobial resistance by this bacterium has led current therapies to lose effectiveness, so alternative treatments are urgently required. Here, we summarize, with a focus on flavodoxin, opportunities for pharmacological intervention offered by the potential protein targets described for this bacterium and provide information on other gastrointestinal pathogens and also on bacteria from the gut microbiota that contain flavodoxin. The process of discovery and development of novel antimicrobials specific for Hp flavodoxin that is being carried out in our group is explained, as it can be extrapolated to the discovery of inhibitors specific for other gastric pathogens. The high specificity for Hp of the antimicrobials developed may be of help to reduce damage to the gut microbiota and to slow down the development of resistant Hp mutants.

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Escherichia coli O8:H8 Carrying a Novel Variant of the Heat-Labile Enterotoxin LT2 Gene Caused Outbreaks of Diarrhea

Cited by 5 publications

References 17 publications

High Prevalence and Persistence of Escherichia coli Strains Producing Shiga Toxin Subtype 2k in Goat Herds

High Prevalence and Persistence of Escherichia coli Strains Producing Shiga Toxin Subtype 2k in Goat Herds

Genomic Characteristics of Stx2e-Producing Escherichia coli Strains Derived from Humans, Animals, and Meats

Flavodoxins as Novel Therapeutic Targets against Helicobacter pylori and Other Gastric Pathogens

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