Escherichia coli O157:H7 and Salmonella Typhimurium adhesion to spinach leaf surfaces: Sensitivity to water chemistry and nutrient availability

Mayton, Holly; Marcus, Ian M.; Walker, Sharon L.

doi:10.1016/j.fm.2018.10.002

Cited by 16 publications

(5 citation statements)

References 53 publications

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“…E. coli O157:H7 and MRSA have also ability to adhere on living or inert surfaces [ 13 , 14 , 15 ], forming amorphous mono or poly microbial structures called biofilm, which is mostly composed of an extracellular polymeric substance of proteins, polysaccharides, nucleic acids, and water [ 13 ]. Biofilm constitutes a resistance strategy that prevents the permeability of the drug and increases drug tolerance hundreds of times [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

et al. 2020

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The emergence of multidrug resistant microorganisms represents a global challenge due to the lack of new effective antimicrobial agents. In this sense, essential oils (EOs) are an alternative to be considered because of their anti-inflammatory, antiviral, antibacterial, and antibiofilm biological activities. Therefore, multiple efforts have been made to consider the potential use of EOs in the treatment of infections which are caused by resistant microorganisms. In this study, 15 EOs of both Colombian and introduced aromatic plants were evaluated against pathogenic strains of E. coli O157:H7 and methicillin resistant Staphylococcus aureus (MRSA) in planktonic and sessile states in order to identify relevant and promising alternatives for the treatment of microbial infections. Forty different compounds were identified in the 15 EO with nine of them constituted mainly by oxygenated monoterpenes (OM). EOs from Lippia origanoides, chemotypes thymol, and carvacrol, displayed the highest antibacterial activity against E. coli O157:H7 (MIC50 = 0.9 and 0.3 mg/mL, respectively) and MRSA (MIC50 = 1.2 and 0.6 mg/mL, respectively). These compounds from EOs had also the highest antibiofilm activity (inhibition percentage > 70.3%). Using scanning electron microscopy (SEM), changes in the size and morphology of both bacteria were observed when they were exposed to sub-inhibitory concentrations of L. origanoides EO carvacrol chemotype. EOs from L. origanoides, thymol, and carvacrol chemotypes represented a viable alternative for the treatment of microbial infections; however, the Selectivity Index (SI ≤ 3) indicated that it was necessary to study alternatives to reduce its in vitro cytotoxicity.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

et al. 2020

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“…Besides, EPS are the components of biofilms, which can control adhesion and protect bacteria from harsh environments (Morales-Garcia, Bailey, Jana, & Burgess, 2019). EPS accumulating on cell surfaces strengthened biofilm adhesion by forming a regulatory membrane (Cheah & Chan, 2021;Tong, Lew, & Derek, 2022;Mayton, Marcus, & Walker, 2019). For example, the accumulation of EPS secreted by E. coli O157:H7 on glass surfaces strengthened the growth and development of biofilm (Oh, Jo, Yang, & Park, 2007).…”

Section: Discussionmentioning

confidence: 99%

The effects of freezing-induced sublethally injury and recovery on adhesion of Escherichia coli O157:H7

Zhai,

Zhang,

Chen

et al. 2023

Preprint

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Freezing as an effective approach for food storage is commonly used in food industry. Notably, Escherichia coli O157:H7 can survive in a sublethally injured state after freezing and recover under suitable conditions, which is a great threat. This research was to investigate changes and mechanism in adhesion of sublethally injured E. coli O157:H7 during freezing and recovery. Adhesion on stainless steel or lettuce surface showed an increase after 16 h-freezing with sublethal ratio of more than 99%. Adhesion recovered to untreated level in recovery on lettuce while didn’t recover on stainless steel. Motility decreased as freezing time increasing, reached minimum after 12 h-freezing and didn’t recover to untreated level under recovery conditions, which was determined by diameter of motility halo. Extracellular polymeric substances production and carbohydrate to protein ratio both reached minimum after 4 h-freezing and then increased. No significant change of LPS structural feature or gene expression was observed during injury and recovery. Besides, the expressions of flagella, pili and EPS related genes were all down-regulated during freezing while were up-regulated after recovery. The results revealed adhesion behavior of freezing-induced sublethally injured E. coli O157:H7, which provides theoretical basis to bacterial prevention.

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“…The adhesion behaviors between bacteria and material surfaces were thermodynamically feasible (Figures S5 and S6), as reported previously. 33 To comprehensively clarify and understand the adsorption process, kinetic studies and adsorption isotherm models were investigated.…”

Section: Kinetic Studies and Adsorptionmentioning

confidence: 99%

Adhesion Mechanisms and Electrochemical Applications of Microorganisms onto a GO-NH₂ Modified Carbon Felt Electrode Material

Cai

Chen

Lü

et al. 2021

Ind. Eng. Chem. Res.

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In this study, a new method comprising graphene oxide (GO) and amino group modifications of carbon felt (CF) electrode material surfaces was employed to improve the initial adhesion capacities of bacteria and their electrochemical performance. The results, which were located at the negative secondary minimum, demonstrated that the electroactive microorganisms (Shewanella xiamenensis BC01 and Escherichia coli DH5a) only adhered reversibly onto the negatively charged CF-GO surfaces. Our study confirmed that the dominant adhesion mechanism for S. xiamenensis on CF-GO was the Lewis acid−base interaction and that for E. coli on CF-GO was the Lifshitz−van der Waals interaction. The results obtained from the electrochemical systems indicated that the positively charged (amino) modification approach, which exhibited improvements in the bacterial adhesion capacities and electrochemical performance, was more effective than the hydrophilic (GO) modification approach. Additionally, our work also provides plausible guidance for preparing electrodes or film-forming materials with remarkable properties.

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Escherichia coli O157:H7 and Salmonella Typhimurium adhesion to spinach leaf surfaces: Sensitivity to water chemistry and nutrient availability

Cited by 16 publications

References 53 publications

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

The effects of freezing-induced sublethally injury and recovery on adhesion of Escherichia coli O157:H7

Adhesion Mechanisms and Electrochemical Applications of Microorganisms onto a GO-NH₂ Modified Carbon Felt Electrode Material

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Escherichia coli O157:H7 and Salmonella Typhimurium adhesion to spinach leaf surfaces: Sensitivity to water chemistry and nutrient availability

Cited by 16 publications

References 53 publications

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

Antimicrobial and Antibiofilm Activities of Essential Oils against Escherichia coli O157:H7 and Methicillin-Resistant Staphylococcus aureus (MRSA)

The effects of freezing-induced sublethally injury and recovery on adhesion of Escherichia coli O157:H7

Adhesion Mechanisms and Electrochemical Applications of Microorganisms onto a GO-NH2 Modified Carbon Felt Electrode Material

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Adhesion Mechanisms and Electrochemical Applications of Microorganisms onto a GO-NH₂ Modified Carbon Felt Electrode Material