Escherichia coli maltose-binding protein activates mouse peritoneal macrophages and induces M1 polarization via TLR2/4 in vivo and in vitro

Ni, Wei; Zhang, Qingyong; Liu, Guomu; Wang, Fang; Yuan, Hongyan; Guo, Yingying; Zhang, Xu; Xie, Fei; Li, Qiongshu; Tai, Guihua

doi:10.1016/j.intimp.2014.04.025

Cited by 23 publications

(22 citation statements)

References 29 publications

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“…We also found that MBP directly promotes U937 cell viability, as well as activating mouse peritoneal macrophages and inducing M1 polarization Ni et al 2014). Further understanding of how MBP activates multiple immune cells may help to explain the adjuvanticity of MBP.…”

Section: Discussionmentioning

confidence: 56%

“…In some reports TLR4 agonist favors Th1 response (Joseph et al 2012;Mark et al 2013), but in others promotes Th2 response (González-Navajas et al 2010). Our previous studies showed that MBP modulated U937 cells viability through TLR2 and activated mouse peritoneal macrophages to M1 type via TLR2/4 Ni et al 2014). Therefore, we supposed that the effects of MBP on CD4 + T cells may be associated with TLR2 and TLR4.…”

Section: Discussionmentioning

confidence: 83%

“…Our previous study found that MBP induced mouse M1 polarization via TLR2/4 (Ni et al 2014). Fernandez also pointed out that MBP induced human DCs maturation via TLR4 (Fernandez et al Fig.…”

Section: Mbp Regulates Tlr2 and Tlr4 Expression Of Purified Cd4 + T Cmentioning

confidence: 91%

“…Several studies have shown that TLR2 agonist, P3CSK4 can induce IFN-␥ secretion of mouse CD4 + T cells in vitro combined with TCR stimulation (Scott et al 2014), whereas TLR4 agonist, low-level of LPS can skew mouse CD4 + T cells to T helper 2 (Th2) response (Stephanie et al 2002). Our previous studies implied that MBP can bind to human monocytes and modulate their viability through TLR2 (Zhao et al 2009); MBP additionally activates mouse peritoneal macrophages and induces M1 polarization via TLR2 and TLR4 (Ni et al 2014). Fernandez also found that MBP induces human dendritic cells (DCs) maturation and the proinflammatory cytokines production via TLR4 (Fernandez et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Further understanding of how MBP activates multiple immune cells may help to explain the adjuvanticity of MBP. An additional study found that MBP directly promotes U937 cell viability, as well as activating mouse peritoneal macrophages and inducing M1 polarization Ni et al 2014). However, the effects of MBP on T cells are largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

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Escherichia coli maltose-binding protein (MBP) directly induces mouse Th1 activation through upregulating TLR2 and downregulating TLR4 expressions

Wang

Liu

et al. 2015

Immunobiology

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 83%

“…Our previous study found that MBP induced mouse M1 polarization via TLR2/4 (Ni et al 2014). Fernandez also pointed out that MBP induced human DCs maturation via TLR4 (Fernandez et al Fig.…”

Section: Mbp Regulates Tlr2 and Tlr4 Expression Of Purified Cd4 + T Cmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Escherichia coli maltose-binding protein (MBP) directly induces mouse Th1 activation through upregulating TLR2 and downregulating TLR4 expressions

Wang

Liu

et al. 2015

Immunobiology

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NZ suppresses TLR4/NF-κB signalings and NLRP3 inflammasome activation in LPS-induced RAW264.7 macrophages

et al. 2015

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Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes

Arenas-Padilla

González-Rascón

Hérnández-Mendoza

et al. 2021

Probiotics & Antimicro. Prot.

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Bifidobacterium animalis subsp. lactis Bb12 is a widely used probiotic that provides numerous health benefits to its host, many due to its immunomodulatory properties. Although the precise mechanism of modulation is still under investigation, several reports associate the interaction of TLR2 with components of the bacterial cell wall inducing a signaling cascade that culminates with the production of cytokines and co-stimulatory molecules. MicroRNAs (miRNAs) have emerged as important post-transcriptional regulators of immune responses, including those toward probiotics. In this study, we analyzed the miRNA expression profile in swine monocytes exposed to Bb12 by using an anti-TLR2 blocking strategy and Bb12 involvement in the regulation of the TLR2 pathway. As a result, the expression of 40 miRNAs was influenced by the treatments (p < 0.01), and 15 differentially expressed miRNAs with validated miRNA-mRNA interactions with around 26 proteins related to the TLR2 pathway were identified. The miRNAs upregulated in response to Bb12 included miR-15a-5p, miR-16-5p, miR-26a-5p, miR-29b-3p, and miR-30d-5p, and the following showed downregulation: miR-181a-5p, miR-19b-3p, miR-21-5p, miR-23a-5p, and miR-221-3p. The expression of let-7c-5p, let-7f-5p, miR-146b-5p, miR-150-5p, and miR-155-5p was increased by Bb12 only when TLR2 was blocked. The identified miRNA common targets were downstream proteins from bacterial recognition via TLR2, such as MyD88, TRAF6, and MAPK members; transcription factors such as NF-κB and AP-1; and cytokines such as IL-6, IL-10, and TNF-α. TLR2 participation was abrogated by anti-TLR2 antibody and suggests that bacterial recognition is complemented by other receptors since there were still changes in the microtranscriptome.

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Escherichia coli maltose-binding protein activates mouse peritoneal macrophages and induces M1 polarization via TLR2/4 in vivo and in vitro

Cited by 23 publications

References 29 publications

Escherichia coli maltose-binding protein (MBP) directly induces mouse Th1 activation through upregulating TLR2 and downregulating TLR4 expressions

Escherichia coli maltose-binding protein (MBP) directly induces mouse Th1 activation through upregulating TLR2 and downregulating TLR4 expressions

NZ suppresses TLR4/NF-κB signalings and NLRP3 inflammasome activation in LPS-induced RAW264.7 macrophages

Immunomodulation by Bifidobacterium animalis subsp. lactis Bb12: Integrative Analysis of miRNA Expression and TLR2 Pathway–Related Target Proteins in Swine Monocytes

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