Escherichia coli in different animal feces: phylotypes and virulence genes

Karakaya, Emre; Aydın, Fuat; Kayman, Tuba; Abay, Seçil

doi:10.1007/s11274-022-03451-w

Cited by 8 publications

(3 citation statements)

References 43 publications

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“…The isolates belonged to two main phylogroups, A and B1, unlike the predominance of B2 which is commonly reported in human pathogenic E. coli , especially the ExPEC but also those of intestinal disease agents. These findings are consistent with other studies [ 18 , 40 , 41 ] that have reported predominance of phylogroups A and B1 in food animals.…”

Section: Discussionsupporting

confidence: 93%

Whole Genome Sequencing Reveals High Genetic Diversity, Diverse Repertoire of Virulence-Associated Genes and Limited Antibiotic Resistance Genes among Commensal Escherichia coli from Food Animals in Uganda

et al. 2023

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Commensal Escherichia coli with broad repertoire of virulence and antimicrobial resistance (AMR) genes pose serious public health risks as reservoirs of AMR and virulence. This study undertook whole genome characterization of commensal E. coli from food-producing animals in Uganda to investigate their genome variability (resistome and virulome). We established that the E. coli had high genomic diversity with 38 sequence types, 24 FimH types, and 33 O-antigen serotypes randomly distributed within three phylogroups (A, B1, and E). A greater proportion (≥93.65%) of the E. coli were resistant to amoxicillin/clavulanate and ampicillin antibiotics. The isolates were AmpC beta-lactamase producers dominated by blaEC-15 (71.88%) and tet(A) (20.31%) antimicrobial resistant genes besides a diverse armory of virulence-associated genes in the class of exotoxin, adhesins, iron uptake, and serine protease autotransporters which varied by host species. Cattle were found to be the major source of E. coli carrying Shiga toxin genes, whereas swine was the main source of E. coli carrying colicin-like Usp toxin gene. The study underscores the importance of livestock as the carrier of E. coli with antimicrobial resistance and a large repertoire of virulence traits with a potential of causing disease in animals and humans by acquiring more genetic traits.

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Section: Discussionsupporting

confidence: 93%

Whole Genome Sequencing Reveals High Genetic Diversity, Diverse Repertoire of Virulence-Associated Genes and Limited Antibiotic Resistance Genes among Commensal Escherichia coli from Food Animals in Uganda

et al. 2023

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“…The DNA was obtained from a pure culture on tryptic soy agar (BD Becton Dickinson and Co., Cuautitlán Izcalli, Mexico) via lysis of a bacterial cell suspension at 95 °C for 15 min, followed by centrifugation at 13,000× g for 3 min. The mdh gene was employed to confirm the identification of E. coli as described by Vasquez et al [ 42 ]. All strains that were confirmed to be E. coli were subjected to the phenotypic screening of ESBL production using the double-disk synergy test according to the European Committee on Antimicrobial Susceptibility Testing guidelines [ 43 ].…”

Section: Methodsmentioning

confidence: 99%

Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated from Food-Producing Animals in Tamaulipas, Mexico

Mandujano,

Cortés-Espinosa,

Vásquez-Villanueva

et al. 2023

Antibiotics

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Extended-spectrum β-lactamase (ESBL)-producing E. coli has become an important global problem for the public health sector. This study aims to investigate the E. coli antimicrobial resistance profile among living food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected from bovines, pigs, chickens and sheep. A total of 5.0% of the strains were phenotypically confirmed as ESBL producers. A high percentage of phenotypic antimicrobial resistance was observed against gentamicin (93.3%), tetracycline (86.6%) and streptomycin (83.3%). The gentamicin-resistant strains showed MDR, distributed among 27 resistance patterns to different antimicrobials. The antimicrobial resistance gene tet(A) was detected in 73.3% of isolates, aadA1 in 60.0% and sul2 in 43.3% of strains. The blaCTX-M gene was found in 23.3% of strains. The virulence gene hlyA was detected in 43.3% of isolates; stx1 and stx2 were not detected in any strain. The phylotyping indicated that the isolates belonged to groups A (33.3%), B1 (16.6%), B2 (40.0%) and D (10.0%). These results show that food-producing animals might be a reservoir of ESBL-producing bacteria and may play a role in their spread.

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“…These two phylogroups are more common in humans (BAILEY et al, 2010) and have been reported at lower frequencies in some animal species than those found in this study (KILANI et al, 2017;XAVIER et al, 2022). This detection raises two hypotheses: either isolates from phylogroups A and D are part of the microbiota of these animals, unlike some wild species already studied (CRISTÓVÃO et al, 2017;ZURFLUH et al, 2019), or there is external influence, such as contamination of riverbeds by human and domestic animal waste or transmission by contaminated feed (AMARSY et al, 2019;GUENTHER et al, 2011;KARAKAYA et al, 2022).…”

Section: Speciesmentioning

confidence: 99%

Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis)

Souza,

Xavier,

Santana

et al. 2024

Cienc. Rural

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The Amazonian manatee (Trichechusinunguis) is an aquatic mammal threatened with extinction. However, few studies have investigated the pathogens in this species, which may affect both animal and human health. This study aimed to evaluate the frequency, distribution, and patterns of antimicrobial susceptibility of Staphylococcus spp. and Escherichiacoli colonizing the nasal and rectal cavities of Amazonian manatees kept in captivity at the National Institute for Amazonian Research (INPA) in the state of Amazonas, Brazil. Rectal and nasal swabs from 44 manatees of different ages were used in this study. The genus Staphylococcus was isolated from the nasal swabs of 32 (72.7%) animals, with two individuals harboring more than one species of Staphylococcus. S. sciuri was the most commonly isolated species. Resistance to penicillin was observed in 13 (40.6%) isolates, more frequent than the other antimicrobials tested (P = 0.01). E. coli was isolated from the rectal swabs of all animals, with phylogroup B1 being the most frequent among the strains obtained (P = 0.0008). Four isolates (6.8%) were positive for virulence factors, three of which were classified as enterotoxigenicE. coli (ETEC) and one as enteropathogenicE. coli (EPEC). To our knowledge, this is the first study to evaluate Staphylococcus spp. and E. coli in Amazonian manatee samples. This study revealed nasal colonization by Staphylococcus spp., mainly S. sciuri, and diarrheagenicE. coli isolates, including antimicrobial-resistant strains.

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Escherichia coli in different animal feces: phylotypes and virulence genes

Cited by 8 publications

References 43 publications

Whole Genome Sequencing Reveals High Genetic Diversity, Diverse Repertoire of Virulence-Associated Genes and Limited Antibiotic Resistance Genes among Commensal Escherichia coli from Food Animals in Uganda

Whole Genome Sequencing Reveals High Genetic Diversity, Diverse Repertoire of Virulence-Associated Genes and Limited Antibiotic Resistance Genes among Commensal Escherichia coli from Food Animals in Uganda

Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated from Food-Producing Animals in Tamaulipas, Mexico

Antimicrobial susceptibility of Staphylococcus sp. and Escherichia coli isolated from captive Amazonian manatee (Trichechus inunguis)

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