Escherichia coli high-risk clone ST410 harboring blaNDM-13 isolated from hospital wastewater in China

Ju, Xiaoyang; Wu, Yantao; Chen, Gongxiang; Zhang, Rong

doi:10.1007/s11356-023-28193-6

Cited by 2 publications

References 17 publications

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Quinolone resistance genesqnr,aac(6′)-Ib-cr,oqxAB, andqepAin environmentalEscherichia coli: insights into their genetic contexts from comparative genomics

Gomi,

Adachi

2024

Preprint

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Previous studies have reported the occurrence of transferable quinolone resistance determinants in environmentalEscherichia coli. However, little is known about their vectors and genetic contexts. To gain insights into these genetic characteristics, we analyzed the complete genomes of 53 environmentalE. coliisolates, including 20 sequenced in this study and 33 sourced from RefSeq. The following transferable quinolone resistance determinants were detected:qnrS1(n = 33),aac(6′)-Ib-cr(n = 12),qnrS2(n = 5),oqxAB(n = 4),qnrB4(n = 3),qnrD1(n = 3),qnrB7(n = 2),qnrB19(n = 2),qepA1(n = 1), andqnrA1(n = 1). These resistance genes were detected on plasmids of diverse replicon types; however,aac(6′)-Ib-cr,qnrS1, andqnrS2were also detected on the chromosome. The genetic contexts surrounding these genes included not only those previously reported in clinical isolates but also novel contexts, such asqnrD1embedded within a composite transposon-like structure bounded by Tn3-derived inverted-repeat miniature elements (TIMEs). This study provides deep insights into mobile genetic elements associated with transferable quinolone resistance determinants, highlighting the importance of genomic surveillance of antimicrobial resistant bacteria in the environment.

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Quinolone resistance genesqnr,aac(6′)-Ib-cr,oqxAB, andqepAin environmentalEscherichia coli: insights into their genetic contexts from comparative genomics

Gomi,

Adachi

2024

Preprint

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Escherichia coli sequence type 410 with carbapenemases: a paradigm shift within E. coli toward multidrug resistance

Pitout,

Peirano,

Matsumura

et al. 2024

Antimicrob Agents Chemother

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Escherichia coli sequence type ST410 is an emerging carbapenemase-producing multidrug-resistant (MDR) high-risk One-Health clone with the potential to significantly increase carbapenem resistance among E. coli . ST410 belongs to two clades (ST410-A and ST410-B) and three subclades (ST410-B1, ST410-B2, and ST410-B3). After a fimH switch between clades ST410-A and ST410-B1, ST410-B2 and ST410-B3 subclades showed a stepwise progression toward developing MDR. (i) ST410-B2 initially acquired fluoroquinolone resistance (via homologous recombination) in the 1980s. (ii) ST410-B2 then obtained CMY-2, CTX-M-15, and OXA-181 genes on different plasmid platforms during the 1990s. (iii) This was followed by the chromosomal integration of bla CMY-2 , fstl YRIN insertion, and ompC/ompF mutations during the 2000s to create the ST410-B3 subclade. (iv) An IncF plasmid “replacement” scenario happened when ST410-B2 transformed into ST410-B3: F36:31:A4:B1 plasmids were replaced by F1:A1:B49 plasmids (both containing bla CTX-M-15 ) followed by bla NDM-5 incorporation during the 2010s. User-friendly cost-effective methods for the rapid identification of ST410 isolates and clades are needed because limited data are available about the frequencies and global distribution of ST410 clades. Basic mechanistic, evolutionary, surveillance, and clinical studies are urgently required to investigate the success of ST410 (including the ability to acquire successive MDR determinants). Such information will aid with management and prevention strategies to curb the spread of carbapenem-resistant E. coli . The medical community can ill afford to ignore the spread of a global E. coli clone with the potential to end the carbapenem era.

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Escherichia coli high-risk clone ST410 harboring blaNDM-13 isolated from hospital wastewater in China

Cited by 2 publications

References 17 publications

Quinolone resistance genesqnr,aac(6′)-Ib-cr,oqxAB, andqepAin environmentalEscherichia coli: insights into their genetic contexts from comparative genomics

Quinolone resistance genesqnr,aac(6′)-Ib-cr,oqxAB, andqepAin environmentalEscherichia coli: insights into their genetic contexts from comparative genomics

Escherichia coli sequence type 410 with carbapenemases: a paradigm shift within E. coli toward multidrug resistance

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