Escherichia coli from Italy Producing OXA-48 Carbapenemase Encoded by a Novel Tn
            <i>1999</i>
            Transposon Derivative

Giani, Tommaso; Conte, Viola; Pilato, Vincenzo Di; Aschbacher, Richard; Weber, Cordula; Larcher, Clara; Rossolini, Gian María

doi:10.1128/aac.00035-12

Cited by 73 publications

(40 citation statements)

References 16 publications

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“…Earlier reports of OXA-48-producing K. pneumoniae and E. coli from geographically diverse locations are associated with a single conserved, epidemic 62-kb IncL/M-type plasmid that harbors no other resistance determinants (2,3,10). While we found conservation of the epidemic plasmid backbone, we also found that the plasmid acquired bla CTX-M-9 .…”

contrasting

confidence: 50%

“…While we found conservation of the epidemic plasmid backbone, we also found that the plasmid acquired bla CTX-M-9 . This likely accounts for the higher cephalosporin and carbapenem MICs seen here compared to those found in previously described OXA-48-producing CPE and OXA-48 J53 transconjugates (2).…”

contrasting

confidence: 35%

“…Plasmid transfer from the CAV1543 took place via mating with Escherichia coli J53 Rif r as previously described (2) Both cases' isolates were found to have additional ␤-lactamases by multiplex PCR and sequencing (5). CAV1543 and J53p1543 were positive for bla CTX-M-9 in addition to bla OXA-48 by PCR and sequencing.…”

mentioning

confidence: 99%

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First Clinical Cases of OXA-48-Producing Carbapenem-Resistant Klebsiella pneumoniae in the United States: the “Menace” Arrives in the New World

et al. 2013

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OXA-48 has emerged as a major carbapenemase associated with the Enterobacteriaceae in Europe, North Africa, and Asia. We report the first two clinical cases of OXA-48-type carbapenemase-producing Enterobacteriaceae in the United States from patients recently hospitalized in Saudi Arabia and India. Each is more carbapenem resistant than nearly all previously reported OXA-48-type-producing Enterobacteriaceae .

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confidence: 50%

contrasting

confidence: 35%

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First Clinical Cases of OXA-48-Producing Carbapenem-Resistant Klebsiella pneumoniae in the United States: the “Menace” Arrives in the New World

et al. 2013

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“…Italy is the country with the highest incidence of OXA-48-producing K. pneumoniae isolates, and has been considered to be the epicenter of the spread of this enzyme in Mediterranean basin [39]. Indeed, several isolates of K. pneumoniae producing OXA-48 have been reported, proving that the situation in this country is becoming endemic [39,40]. In addition, Pirš et al reported the first case of OXA-48-producing K. pneumoniae in Slovenia, isolated from a rectal swab collectedfrom a patient transferred from Libya.…”

Section: Indeed Mamlouk Et Al Detected the Blactx-m-15 Gene Inmentioning

confidence: 99%

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Mathlouthi

Al-Bayssari

Salabi

et al. 2016

J Infect Dev Ctries

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Introduction: The aim of the study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among clinical isolates of Enterobacteriaceae recovered from Tunisian and Libyan hospitals. Methodology: Bacterial isolates were recovered from patients in intensive care units and identified by biochemical tests and MALDI-TOF. Antibiotic susceptibility testing was performed by disk diffusion and the E-test method. ESBL and carbapenemase activities were detected using standard microbiological tests. Antibiotic resistance-encoding genes were screened by PCR and sequencing. Clonal relationships between Klebsiella pneumoniae strains were carried out using multi-locus sequence typing (MLST). Results: A total of 87 isolates were characterized, with 51 and 36, respectively, identified as E. coli and K. pneumoniae. Overall the resistance prevalence was high for aminoglycosides (> 60%), fluoroquinolones (> 80%), and extended-spectrum cephalosporins (> 94%), and was low for imipenem (11.4%). Among this collection, 58 strains (66.6%) were ESBL producers and 10 K. pneumoniae strains (11.4%) were carbapenemase producers. The antibiotic resistance-encoding genes detected were blaCTX-M-15 (51.7%), blaTEM-1 (35.6%), several variants of blaSHV (21.8%), and blaOXA-48 (11.4%). The MLST typing of K. pneumoniae isolates revealed the presence of multiple clones and three novel sequence types. Also, close relationships between the OXA-48-producing strains from Tunisia and Libya were demonstrated. Conclusions: This study is the first paper describing the emergence of carbapenemase-and ESBL-producing Enterobacteriaceae, sensitive to colistin, isolated in Tunisia and Libya. Active surveillance and testing for susceptibility to colistin should be implementing because resistance to colistin, mainly in Klebsiella, has been recently reported worldwide.

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“…Among the multi resistant traits, extended-spectrum beta-lactamases and carbapenemases, particularly in Gram-negative bacilli, are a present public health concern (Struelens et al 2010;Giani et al 2009Giani et al , 2012Giani et al , 2013Monteiro et al 2012;Fontana et al 2010;Perilli et al 2008). In Italy, non-carbapenem-susceptible K. pneumoniae was first reported in 2009 and has since spread to alarming levels (Giani et al 2009(Giani et al , 2013Fontana et al 2010).…”

Section: Discussionmentioning

confidence: 95%

Multiplex real-time PCR probe-based for identification of strains producing: OXA48, VIM, KPC and NDM

Favaro

Sarti

Fontana

2014

World J Microbiol Biotechnol

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The spread of multi-resistant enterobacteria, particularly carbapenem-resistant Enterobacteriaceae (CRE), in both community and hospital settings is a global problem. The phenotypic identification of CRE is complex, occasionally inconclusive and time consuming. However, commercially available molecular assays are very expensive, and many do not allow the simultaneous identification of all genetic markers of resistance that have been recognised in CRE (bla KPC, bla OXA-48, bla VIM and bla NDM). The aim of the present study is to describe a new test: a multiplex real time PCR probe-based assay designed for the simultaneous detection of KPC, OXA-48, VIM and NDM in a short time (no longer than 90 min from the extraction of DNA to detection). Our assay correctly identified 63 CRE isolates and all standard reference strains tested, in agreement with and extending the results of phenotypic identification tests; additionally, a KPC-VIM co-expressing Enterobacter aerogenes isolate was identified using the new assay, whereas traditional methods failed to detect it. The assay was also able to correctly detect 28 CRE-producers from 50 positive blood cultures, again detecting, in four specimens, the presence of CRE co-expressing KPC and VIM, which were only partially identified by traditional methods. Finally, when used directly on rectal swabs, the assay enabled the identification of CRE-carrier patients, for whom isolation is mandatory in a hospital setting.

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Escherichia coli from Italy Producing OXA-48 Carbapenemase Encoded by a Novel Tn 1999 Transposon Derivative

Cited by 73 publications

References 16 publications

First Clinical Cases of OXA-48-Producing Carbapenem-Resistant Klebsiella pneumoniae in the United States: the “Menace” Arrives in the New World

First Clinical Cases of OXA-48-Producing Carbapenem-Resistant Klebsiella pneumoniae in the United States: the “Menace” Arrives in the New World

Carbapenemases and extended-spectrum β-lactamases producing Enterobacteriaceae isolated from Tunisian and Libyan hospitals

Multiplex real-time PCR probe-based for identification of strains producing: OXA48, VIM, KPC and NDM

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