Escherichia coli enteropatogênica: uma categoria diarreiogênica versátil

Souza, Cintya de Oliveira; Melo, Thainara Roberta Barros; Melo, Caroline do Socorro Barros; Menezes, E. M.; Carvalho, Aline Correa de; Monteiro, Leni Célia Reis

doi:10.5123/s2176-62232016000200010

Cited by 13 publications

(8 citation statements)

References 19 publications

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“…A Gramnegativa é responsável pelas infecções de pneumonia, peritonite, infecções no aparelho urinário, infecções em feridas e a meningite. Já a Gram-positiva causa carbúnculo, difteria, listeriose, erisipelotricose (LIMA et al, 2015;SOUZA et al, 2016).…”

Section: Atividade Antimicrobianaunclassified

Atividades Antioxidante E Antimicrobiana Em Hidrolisados De Pele De Rã-Touro

et al. 2021

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A indústria e a ciência estudam e desenvolvem produtos a partir de fonte natural para suprir a demanda por moléculas com atividades antioxidante e antimicrobiana. A pele de rã é um subproduto de frigoríficos de abate desse animal e tem sido objeto de estudo na prospecção de peptídeos bioativos oriundos de hidrólise enzimática. No Brasil, a rã-touro (Lithobates catesbeianus) é a única espécie permitida para exploração comercial em ranários para aproveitamento da carne. Este estudo teve por objetivo avaliar o uso do ultrassom em conjunto com a hidrólise enzimática de pele de rã-touro para a produção de peptídeos com atividades antioxidante e antimicrobiana. Os resultados mostraram que os peptídeos liberados pela hidrólise convencional da pele de rã-touro possuem expressiva atividade antioxidante. O uso de um sonicador diminuiu o tempo de hidrólise para 5 min, com a melhor potência de 250 W avaliada pelas análises do DPPH e do FRAP, enquanto que a potência de 100 W foi melhor para a análise do ABTS. Em relação à atividade antimicrobiana, os hidrolisados não apresentaram qualquer resultado na concentração de 20 mg mL-1, sendo necessário fazer outros estudos com valores maiores de concentração de amostra.

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Section: Atividade Antimicrobianaunclassified

Atividades Antioxidante E Antimicrobiana Em Hidrolisados De Pele De Rã-Touro

et al. 2021

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“…Enteropathogenic E. coli (EPEC), and shiga toxin-producing E. coli (STEC) and enterohemorrhagic E. coli (EHEC) have been declared as major causes of diarrhea since 1940 and continue to be related to sporadic cases of outbreaks (C. D. O. Souza et al, 2016;Gomes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Hygienic-health quality and microbiological hazard of clandestine Minas Frescal cheese commercialized in north Tocantins, Brazil

Oliveira

Santos

Dias

et al. 2021

Semina: Ciênc. Agrár.

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Cheese is a popular product that integrates the diet of the majority of the population, almost on a daily basis. It is rich in nutrients and, therefore, also an excellent substrate for the multiplication of microorganisms, including pathogens. The microbiological contamination of these products is highly relevant to the industry, resulting in an economic loss, and to public health, due to the risk of causing foodborne diseases. The aim of this study was to evaluate the hygienic-sanitary quality and the presence of bacterial pathogens in the clandestine Minas Frescal cheeses sold in the street open markets of Araguaína, TO, Brazil. Twenty-one samples were collected to evaluate the presence of total (TC) and thermotolerant (TTC) coliforms, Escherichia coli, and the pathotypes enteropathogenic E. coli (EPEC), and shiga toxin-producing E. coli (STEC) and enterohemorrhagic E. coli (EHEC), coagulase-positive staphylococci (CPS), Salmonella spp., and Listeria monocytogenes. The results showed that 100% of the cheese samples were in disagreement with the maximum standards of TC and TTC defined in the Brazilian legislation that regulates food quality control. In addition, 85.71% of the samples had values above the standard Brazilian maximum limit. In the study of diarrheagenic E. coli, 52.38%, 66.6%, and 4.76% of the samples were positive for EPEC, STEC, and EHEC, respectively, indicating fecal origin contamination of the samples and a potential consumer risk. No Salmonella spp. or Listeria monocytogenes were detected in these cheese samples. The high count of total and thermotolerant coliforms found in the samples demonstrates unsatisfactory sanitary conditions in the production, storage, and/or commercialization of this food product. The presence of EPEC, STEC, EHEC, and coagulase-positive staphylococci at high concentrations shows the health risk of the imminent consumption of Minas Frescal cheese.

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“…There are six pathogenic E. coli pathotypes, collectively known as diarrheagenic E. coli (DEC). They have a predilection for the host's enteral cells, causing intestinal infection and they are classified as: enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli (EHEC) or Shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC) and diffusely adherent E. coli (DAEC) (Souza et al, 2016). EPEC cause childhood diarrhea and its action mechanism is through the destruction of small intestine microvillus.…”

Section: Introductionmentioning

confidence: 99%

Genotypical characterization of thermotolerant coliforms isolated from food produced by a Solidarity Economic Venture of Bahia (Brazil)

et al. 2021

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Many Solidarity Economic Venture (SEV) are family farmers who seek to add value to production through artisanal processing, which can lead to food contamination. Thus, this study aimed to genotypically characterize thermotolerant coliforms (TtC) strains from food produced by local agribusinesses of SEV during January to April 2019. Samples from thirteen production units (PU) from the SEV were submitted to a microbiological analysis of thermotolerant coliforms (AFNOR 3M1/2 – 09/89), using a fast count method in Petrifilm™ dishes. The Polymerase Chain Reaction (PCR) technique was used to verify the following virulence genes (VGs) associated with Escherichia coli: stx, typical from enterohemorrhagic E. coli (EHEC); bfpA typical from entheropathogenic E. coli (EPEC) and elt and slt, typical from entherotoxigenic E. coli (ETEC). The results showed that two samples of queijadinha (typical Brazilian candy made with eggs and coconut) and one sample of cassava cake presented characteristic colonies TtC. This way, three strains were isolated in order to perform the PCR technique. However, the genes used in the reaction were not detected in the isolated strains. Therefore, it is suggested that the isolated strains are from E. coli pathotypes with different virulence genes than the ones analyzed belong other types of TtC, such as Enterobacter and Klebsiella. Although the virulence of genes has not been confirmed, the presence of TtC on food indicates hygiene flaws during production and, therefore, measurements to control and prevent contamination should be taken.

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Escherichia coli enteropatogênica: uma categoria diarreiogênica versátil

Cited by 13 publications

References 19 publications

Atividades Antioxidante E Antimicrobiana Em Hidrolisados De Pele De Rã-Touro

Atividades Antioxidante E Antimicrobiana Em Hidrolisados De Pele De Rã-Touro

Hygienic-health quality and microbiological hazard of clandestine Minas Frescal cheese commercialized in north Tocantins, Brazil

Genotypical characterization of thermotolerant coliforms isolated from food produced by a Solidarity Economic Venture of Bahia (Brazil)

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