Escherichia coli Deoxyribonucleic Acid Polymerase I, a Zinc Metalloenzyme

Springgate, Clark F.; Mildvan, Albert S.; Abramson, Ronald; Engle, James L.; Loeb, Lawrence A.

doi:10.1016/s0021-9258(19)43497-2

Cited by 189 publications

(32 citation statements)

References 21 publications

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“…The stimulus for these studies was the kinetic experiments of Chang and Bollum suggesting a role for zinc in catalysis by the terminal deoxynucleotidyltransferase of calf thymus (19) and our direct demonstration of the presence of zinc in homogeneous DNA polymerases from E . coli, sea urchin nuclei (6), and bacteriophage T4 (5) .…”

Section: Discussionmentioning

confidence: 99%

“…Most important, enzyme-bound zinc (1 g atom per mol of enzyme) is required for catalysis by E. coli DNA polymerase I (5) . The removal of Zn++ is accompanied by a proportional loss of polymerase activity, an effect which can be entirely reversed by incubating the apoenzyme with zinc (5) . Other DNA polymerases from chicken embryos (20) and from Rous' sarcoma virus (21) have been reported to be inhibited by o-phen .…”

Section: Discussionmentioning

confidence: 99%

“…At the start of incubation 0 .025 ml of phytohemagglutinin "M" (General Biochemicals Div ., Mogul Corp ., Chagrin Falls, Ohio) was added to each culture . The complete culture medium including serum contained 1-2 pM zinc as determined by atomic adsorption spectroscopy (5) .…”

Section: Lymphocyte Culturesmentioning

confidence: 99%

“…The incorporation of thymidine into DNA is impaired in livers of rats maintained on a zinc-deficient diet (1) : Zn++ appears to be required for the induction of DNA synthesis in kidney cultures (2) and in liver cells after partial hepatectomy of rats (3) as well as for the continuation of DNA synthesis in cultured chick embryos (4) . In addition, zinc is required for catalysis by Escherichia coli DNA polymerase 1 (5) . It is present in homogeneous preparations of DNA polymerases from E. coli, sea urchin nuclei (6), and T4 bacteriophage (5) .…”

Section: Introductionmentioning

confidence: 99%

“…In addition, zinc is required for catalysis by Escherichia coli DNA polymerase 1 (5) . It is present in homogeneous preparations of DNA polymerases from E. coli, sea urchin nuclei (6), and T4 bacteriophage (5) .…”

Section: Introductionmentioning

confidence: 99%

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Zinc Requirement for Dna Replication in Stimulated Human Lymphocytes

Williams

Loeb

1973

The Journal of Cell Biology

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114

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The requirement for Zn++ in DNA replication by phytohemagglutinin-stimulated human lymphocytes was studied . When 6 µM o-phenanthroline, a chelator with a high affinity for Zn++, is added to cultures of stimulated lymphocytes a nearly complete inhibition of thymidine incorporation results within a few hours . In contrast, the incorporation of uridine is only slightly reduced and the incorporation of leucine is unaffected . m-Phenanthroline, a nonchelating analogue, does not alter the rate of thymidine incorporation even when present in 10-fold greater amounts than o-phenanthroline . The inhibition of thymidine incorporation by o-phenanthroline could be entirely reversed by the addition of Zn++ to the cultures, or could be prevented by the prior addition of either Zn++ or Ni++ . All other divalent cations tested were incapable of reversing the o-phenanthroline inhibition of thymidine incorporation .

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Lymphocyte Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Zinc Requirement for Dna Replication in Stimulated Human Lymphocytes

Williams

Loeb

1973

The Journal of Cell Biology

Self Cite

114

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A Syndrome of Acute Zinc Loss

Henkin¹,

Patten²,

Re³

et al. 1975

Arch Neurol

261

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Oral administration of the amino acid histidine to six patients with progressive systemic sclerosis produced anorexia, taste ans smell dysfunction, changes in mentation, and cerebellar dysfunction in each patient; these changes were associated with significant decreases in serum zinc concentration and significant increases in urinary zinc excretion. Administration of zinc ion, even with continued histidine administration, returned each of the signs and symptoms to or toward normal within 8 to 24 hours in each patient at the same time that correction of the serum zinc concentration occurred. The signs and symptoms noted constitute a syndrome related to acute zinc loss.

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Artificial Nucleases

et al. 2001

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The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).

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Escherichia coli Deoxyribonucleic Acid Polymerase I, a Zinc Metalloenzyme

Cited by 189 publications

References 21 publications

Zinc Requirement for Dna Replication in Stimulated Human Lymphocytes

Zinc Requirement for Dna Replication in Stimulated Human Lymphocytes

A Syndrome of Acute Zinc Loss

Artificial Nucleases

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