Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function

Abstract

Study design: Clonal typing of neurogenic clones. Objective: To determine whether neurogenic clones carried over weeks in the urine of asymptomatic children with neurogenic bladder were similar to known uropathogenic clones associated with disease. Setting: Michigan State University; VA Medical Center, Minneapolis, MN, USA. Methods: Escherichia coli isolates from the urine of 15 children previously followed were typed by multilocus sequence typing and compared to 2 human pyelonephritis genome strains, 29 pediatric or adult symptomatic urinary tract infection strains, 15 pediatric asymptomatic bacteriuria strains, 6 animal urinary tract infection strains and a neonatal meningitis-septicemia prototype K1 strain. Phylotypes and virulence genotypes were determined using PCR. Results: Twenty-nine E. coli isolates were classified into 15 clones. Six of 15 clones were the same sequence type or a close relative to a clone that caused disease in a human or animal. These clones were considered uropathogens. The remaining nine clones were not closely related to a clone that caused disease and were considered commensal clones. Uropathogens were predominantly group B2, exhibited more virulence genes and were carried for more weeks in the neurogenic bladder compared to commensal clones. Nine of 15 children carried one or more uropathogenic clones; 4 children carried one or more commensal clones and 2 children carried both uropathogenic and commensal clones. Conclusion: Children with neurogenic bladder most commonly carried commensal clones. Uropathogenic clones were associated with prolonged carriage, however, carriage was not associated with symptomatic disease or deterioration of the upper urinary tract.

Genetic Evaluation ofE. coliStrains Isolated from Asymptomatic Children with Neurogenic Bladders

“…A low percentage of the strains were the A or D phylotype. It is interesting to note that the proportions of all four phylotypes are comparable to those reported in another study of E. coli isolated from asymptomatic pediatric patients with neurogenic bladders [ 24 ]. It appears that among E. coli that are capable of long-term bladder colonization, many have a phylotype similar to that of both UPEC and commensal strains.…”

“…For comparison, the prevalence of E. coli phylotypes identified in other published studies is also included in each table. These prior studies have investigated E. coli in the pediatric neurogenic bladder [ 24 ], commensal E. coli strains [ 10 , 12 , 13 , 25 ], and E. coli from symptomatic UTIs [ 10 , 11 ]. Strains in the current study were similar to another study of E. coli colonizing urine of pediatric patients with neurogenic bladders [ 24 ].…”

Nonsurgical Management of the Neurogenic Bladder in Children

“…These are not suitable organisms to colonize the bladder and should be eradicated to minimize formation of urinary tract stones or biofilms [13]. In general, other bacteria, such as E. coli and Klebsiella sp., can safely colonize the bladder [14,15].…”

Physicochemical and antimicrobial properties of natural rubber latex films in the presence of vegetable oil microemulsions

“…For example, in most cases, E. coli causes urinary tract infections but extraintestinal pathogenic E . coli (ExPEC) was capable of causing meningitis . S. aureus which commonly found to cause minor skin infections can also be associated with the infective endocarditis disease as reported elsewhere …”