Escherichia Coli Associated with Neonatal Diarrhoea in Piglets and Calves

Guiñée, P. A. M.; Jansen, W. H.; Wadström, Torkel; Sellwood, R.

doi:10.1007/978-94-009-8328-1_18

Cited by 91 publications

(67 citation statements)

References 44 publications

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“…The determination of O and H antigens was carried out by the method described by Guinée et al (14) employing all available O (O1 to O181) and H (H1 to H56) antisera. All antisera were obtained and absorbed with the corresponding cross-reacting antigens to remove the nonspecific agglutinins.…”

Section: Methodsmentioning

confidence: 99%

Serotypes, Virulence Genes, and Intimin Types ofShiga Toxin (Verotoxin)-Producing Escherichia coli Isolatesfrom Cattle in Spain and Identification of a New Intimin VariantGene( eae- ξ)

et al. 2004

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A total of 514 Shiga toxin-producing Escherichia coli (STEC) isolates from diarrheic and healthy cattle in Spain were characterized in this study. PCR showed that 101 (20%) isolates carried stx 1 genes, 278 (54%) possessed stx 2 genes, and 135 (26%) possessed both stx 1 and stx 2 . Enterohemolysin (ehxA) and intimin (eae) virulence genes were detected in 326 (63%) and in 151 (29%) of the isolates, respectively. STEC isolates belonged to 66 O serogroups and 113 O:H serotypes (including 23 new serotypes). However, 67% were of one of these 15 serogroups (O2, O4, O8, O20, O22, O26, O77, O91, O105, O113, O116, O157, O171, O174, and OX177) and 52% of the isolates belonged to only 10 serotypes (O4:H4, O20:H19, O22:H8, O26:H11, O77:H41, O105:H18, O113:H21, O157:H7, O171:H2, and ONT:H19). Although the 514 STEC isolates belonged to 164 different seropathotypes (associations between serotypes and virulence genes), only 12 accounted for 43% of isolates. Seropathotype O157:H7 stx 2 eae-␥1 ehxA (46 isolates) was the most common, followed by O157:H7 stx 1 stx 2 eae-␥1 ehxA (34 isolates), O113:H21 stx 2 (25 isolates), O22:H8 stx 1 stx 2 ehxA (15 isolates), O26:H11 stx 1 eae-␤1 ehxA (14 isolates), and O77:H41 stx 2 ehxA (14 isolates). Forty-one (22 of serotype O26:H11) isolates had intimin ␤1, 82 O157:H7 isolates possessed intimin ␥1, three O111:H-isolates had intimin type ␥2, one O49:Hstrain showed intimin type ␦, 13 (six of serotype O103:H2) isolates had intimin type and eight (four of serotype O156:H-) isolates had intimin . We have identified a new variant of the eae intimin gene designated (xi) in two isolates of serotype O80:H-. The majority (85%) of bovine STEC isolates belonged to serotypes previously found for human STEC organisms and 54% to serotypes associated with STEC organisms isolated from patients with hemolytic uremic syndrome. Thus, this study confirms that cattle are a major reservoir of STEC strains pathogenic for humans.

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Section: Methodsmentioning

confidence: 99%

Serotypes, Virulence Genes, and Intimin Types ofShiga Toxin (Verotoxin)-Producing Escherichia coli Isolatesfrom Cattle in Spain and Identification of a New Intimin VariantGene( eae- ξ)

et al. 2004

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“…O-and H-antigen determinations were carried out by the method described by Guinée et al (31) by using all available type O (O1 to O181) and type H (H1 to H56) antisera in the Laboratorio de Referencia de E. coli, Lugo, Spain. All antisera were obtained and absorbed with the corresponding cross-reacting antigens to remove the nonspecific agglutinins.…”

Section: Methodsmentioning

confidence: 99%

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157:H7 Isolates from Humans and Animals in Spain: Identification and Characterization of Two Predominating Phage Types (PT2 and PT8)

Mora

Blanco

et al. 2004

J Clin Microbiol

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Phage typing and DNA macrorestriction fragment analysis by pulsed-field electrophoresis (PFGE) were used for the epidemiological subtyping of a collection of Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains isolated in Spain between 1980 and 1999. Phage typing distinguished a total of 18 phage types among 171 strains isolated from different sources (67 humans, 82 bovines, 12 ovines, and 10 beef products). However, five phage types, phage type 2 (PT2; 42 strains), PT8 (33 strains), PT14 (14 strains), PT21/28 (11 strains), and PT54 (16 strains), accounted for 68% of the study isolates. PT2 and PT8 were the most frequently found among strains from both humans (51%) and bovines (46%). Interestingly, we detected a significant association between PT2 and PT14 and the presence of acute pathologies. A group of 108 of the 171 strains were analyzed by PFGE, and 53 distinct XbaI macrorestriction patterns were identified, with 38 strains exhibiting unique PFGE patterns. In contrast, phage typing identified 15 different phage types. A total of 66 phage type-PFGE subtype combinations were identified among the 108 strains. PFGE subtyping differentiated between unrelated strains that exhibited the same phage type. The most common phage type-PFGE pattern combinations were PT2-PFGE type 1 (1 human and 11 bovine strains), PT8-PFGE type 8 (2 human, 6 bovine, and 1 beef product strains), PT2-PFGE subtype 4A (1 human, 3 bovine, and 1 beef product strains). Nine (29%) of 31 human strains showed phage type-PFGE pattern combinations that were detected among the bovine strains included in this study, and 26 (38%) of 68 bovine strains produced phage type-PFGE pattern combinations observed among human strains included in this study, confirming that cattle are a major reservoir of strains pathogenic for humans. PT2 and PT8 strains formed two groups which differed from each other in their motilities, stx genotypes, PFGE patterns, and the severity of the illnesses that they caused.

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“…Colony blots were prepared, processed, and hybridized under high-stringency conditions as described previously (36 Serotyping. Determination of O and H antigens was carried out as described previously (18,34) with all available O-antigen (O1 to O181) and H-antigen (H1 to H56) antisera.…”

Section: Methodsmentioning

confidence: 99%

Virulence Markers of Enteroaggregative Escherichia coli Isolated from Children and Adults with Diarrhea in Brasília, Brazil

et al. 2003

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Escherichia coli strains isolated from sporadic cases of acute diarrhea in children and adults and from children without diarrhea were investigated for the presence of the pAA plasmid. Strains harboring the pAA plasmid were isolated at similar frequencies from children with (19.6%) and without (10.8%) diarrhea and from adults with diarrhea (11.8%). The genotypic and phenotypic virulence markers of these strains were further analyzed. Most of the strains were positive for EAST1 (73%), and this toxin was detected significantly more frequently in strains from children with diarrhea than in strains from adults with diarrhea (P < 0.05). Likewise, pic sequences were detected significantly more frequently in strains from children with diarrhea than in strains from adults with diarrhea (P < 0.005) and controls (P < 0.025). Furthermore, the association of pAA positivity (pAA ؉ ) and pic positivity (pic ؉ ) was more frequently found for strains from children with diarrhea than for strains from controls, indicating that pAA ؉ pic ؉ strains may represent a subset of pAA ؉ strains associated with disease in children. Most of the strains (82.5%) adhered to cells presenting the typical aggregative pattern. The frequency of occurrence of enteropathogenic E. coli (EPEC) serogroups in the strains from children with diarrhea was very high (56%), while none of the strains from adults with diarrhea belonged to EPEC serogroups. Extraintestinal virulence markers were very commonly found in strains from adults with diarrhea. The frequencies of occurrence of the adhesins AFA and SFA were significantly higher in strains from adults with diarrhea than in strains from children with diarrhea. More than one extraintestinal virulence marker was found in 58% of the strains from adults with diarrhea but in only 7.7% of the strains from children with diarrhea. Our results show that pAA ؉ strains isolated from children and adults with diarrhea present very different profiles when enteroaggregative E. coli virulence markers, serotypes, and extraintestinal virulence markers are considered.Several studies have reported on the association of enteroaggregative Escherichia coli (EAEC) with diarrheal disease in children (3,4,13,17,21,23,33). The EAEC pathotype has been defined by its aggregative pattern of adherence to tissue culture cells, but this definition seems to cover heterogeneous groups of strains, as indicated in volunteer studies (26) and investigations of the natural occurrence of the disease (3, 13, 14, 17). Nataro et al. (26) carried out a volunteer study with four different EAEC strains and showed that three of them failed to elicit diarrhea. Several epidemiological studies reported on an association of EAEC strains with sporadic diarrhea, either acute or persistent, and outbreaks in different geographic locations (3,13,17,21,23,33). Furthermore, Steiner et al. (44) reported on an association of EAEC with growth impairment even in the absence of diarrheal symptoms. However, in some case-control investigations, EAEC strains were isolated...

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Escherichia Coli Associated with Neonatal Diarrhoea in Piglets and Calves

Cited by 91 publications

References 44 publications

Serotypes, Virulence Genes, and Intimin Types ofShiga Toxin (Verotoxin)-Producing Escherichia coli Isolatesfrom Cattle in Spain and Identification of a New Intimin VariantGene( eae- ξ)

Serotypes, Virulence Genes, and Intimin Types ofShiga Toxin (Verotoxin)-Producing Escherichia coli Isolatesfrom Cattle in Spain and Identification of a New Intimin VariantGene( eae- ξ)

Phage Types and Genotypes of Shiga Toxin-Producing Escherichia coli O157:H7 Isolates from Humans and Animals in Spain: Identification and Characterization of Two Predominating Phage Types (PT2 and PT8)

Virulence Markers of Enteroaggregative Escherichia coli Isolated from Children and Adults with Diarrhea in Brasília, Brazil

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