Escaping to the summits: Phylogeography and predicted range dynamics of Cerastium dinaricum, an endangered high mountain plant endemic to the western Balkan Peninsula

Kutnjak, Denis; Kuttner, Michael; Niketić, Marjan; Dullinger, Stefan; Schönswetter, Peter

doi:10.1016/j.ympev.2014.05.015

Cited by 53 publications

(70 citation statements)

References 53 publications

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“…The ndhJ-trnT region yielded the highest variability and was thus sequenced in this study. PCR and sequencing of ITS and the plastid ndhJ-trnT region were performed for one to two individuals per population (Table 1) as described by Kutnjak et al (2014), with the exception that sequencing was carried out at Eurofins Genomics (Ebersberg, Germany). Contigs were assembled, edited and sequences were aligned using Geneious Pro 5.5.9 (Kearse et al 2012).…”

Section: Sequencing and Phylogenetic Analyses Of Its And Ndhj-trntmentioning

confidence: 99%

“…The RGS of silica gel-dried leaves of C. subtriflorum and C. sylvaticum was estimated using flow cytometry as described by Kutnjak et al (2014) for one to five individuals per population (Table 1). Despite the fact that the coefficient of variation (CV) of the G0/G1 peak of the sample exceeded the 5% threshold in four populations, we included these data in further analyses as the peaks were clearly visible and the RGS values highly similar to the RGS values obtained in other populations.…”

Section: Relative Genome Size (Rgs) Estimation and Statistical Analysesmentioning

confidence: 99%

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Diversification of Cerastium sylvaticum and C. subtriflorum on the margin of the south-eastern Alps

Skubic

Schönswetter

2018

Plant Syst Evol

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The south-eastern Alps and the southerly adjacent areas are considered an important refugium for plant species during the Pleistocene glaciations; consequently, they harbour many endemic taxa. One of them is Cerastium subtriflorum, which in this area occurs sympatrically with morphologically similar, but more widespread C. sylvaticum. Here, we used amplified fragment length polymorphisms as well as ITS and plastid DNA sequences to explore phylogenetic relationships between the two species as well as their relationships to other Cerastium taxa previously deemed closely related. Our data show that both species are genetically well-differentiated sister taxa, but their relationship to other taxa remains unclear; both species rather form an independent evolutionary lineage within European Cerastium. Cerastium sylvaticum exhibits low genetic differentiation among populations in the investigated area, suggesting a single Pleistocene refugium in that part of Europe. The much more pronounced genetic differentiation within C. subtriflorum is likely a result of multiple Pleistocene refugia or more recent vicariance among the three disjunct partial distribution areas. Phylogenetic sequence data suggest hybridisation between the two taxa, resulting in a discordant phylogenetic position between ITS and plastid trees of one population of C. subtriflorum. Flow cytometric and multivariate morphometric analyses show that C. subtriflorum and C. sylvaticum differ in their genome size as well as in multiple morphological traits. We provide a taxonomic treatment for both species including an identification key and species descriptions.

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Section: Sequencing and Phylogenetic Analyses Of Its And Ndhj-trntmentioning

confidence: 99%

Section: Relative Genome Size (Rgs) Estimation and Statistical Analysesmentioning

confidence: 99%

Diversification of Cerastium sylvaticum and C. subtriflorum on the margin of the south-eastern Alps

Skubic

Schönswetter

2018

Plant Syst Evol

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“…We included four populations of Cerastium dinaricum previously analysed by Kutnjak et al (2014), including the population from the type locality, and additionally sampled four populations from Albania tentatively ascribed to C. dinaricum s.l., including the population from the type locality of C. hekuravense (Table 1, Fig. 1).…”

Section: Plant Materialsmentioning

confidence: 99%

“…AFLP fingerprinting was conducted as described by Kutnjak et al (2014). Two blanks (DNA replaced by water) were included to test for contamination, and four samples were used as replicates between the two PCR batches to test the reproducibility.…”

Section: Aflp Analysesmentioning

confidence: 99%

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Long neglected diversity in the Accursed Mountains of northern Albania: Cerastium hekuravense is genetically and morphologically divergent from C. dinaricum

2017

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using flow cytometry, differ. We propose a taxonomic treatment with lectotype designation for both taxa and provide descriptions and an identification key. Last but not least, these cold-adapted species mostly growing on northerly exposed humid screes are highly threatened due to the global warming and should be ranked endangered according to IUCN criteria. Cerastium hekuravense known only from three localities is likely one of the most endangered mountain plant species of the Balkan Peninsula.

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High genetic and morphological diversification of the Euphorbia verrucosa alliance (Euphorbiaceae) in the Balkan and Iberian peninsulas

Caković

Cresti

Stešević

et al. 2021

TAXON

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We explored the diversification of the southern European Euphorbia verrucosa alliance applying molecular (amplified fragment length polymorphism fingerprinting [AFLP], sequencing of the nuclear ribosomal internal transcribed spacer), karyological (relative genome size estimations, chromosome counts) and morphometric methods. The AFLP data inferred four main phylogenetic lineages corresponding to western-southern Balkan E. montenegrina, central Balkan E. serpentini, northern Balkan-central European-north Italian E. verrucosa and Iberian-southern French E. flavicoma. Genetic diversification is strongest within the Iberian and the Balkan peninsulas, suggesting Pleistocene persistence of the species in different micro-refugia. In contrast, weak genetic structure in E. verrucosa suggests Holocene (after last glacial maximum) expansion to central and western Europe, likely from a northern Balkan refugium. Karyological data provide evidence for tetraploidisation events in E. flavicoma and E. montenegrina, but not in E. verrucosa and E. serpentini. By integrating phylogenetic data with multivariate morphometric analyses, we propose a new taxonomic treatment for this group, mainly by recognising the Balkan endemics E. montenegrina and E. serpentini as independent species and by redefining the distributions of E. flavicoma and E. verrucosa. Our study underlines the importance of the Balkan and Iberian peninsulas as major Pleistocene refugia.

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Escaping to the summits: Phylogeography and predicted range dynamics of Cerastium dinaricum, an endangered high mountain plant endemic to the western Balkan Peninsula

Cited by 53 publications

References 53 publications

Diversification of Cerastium sylvaticum and C. subtriflorum on the margin of the south-eastern Alps

Diversification of Cerastium sylvaticum and C. subtriflorum on the margin of the south-eastern Alps

Long neglected diversity in the Accursed Mountains of northern Albania: Cerastium hekuravense is genetically and morphologically divergent from C. dinaricum

High genetic and morphological diversification of the Euphorbia verrucosa alliance (Euphorbiaceae) in the Balkan and Iberian peninsulas

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