ESBL‐production in Escherichia coli and Klebsiella pneumoniae isolates from Nigeria

Hertz, Frederik Boëtius; Jansåker, Filip; Okon, Kenneth O.; Abdulmumin, Ibrahim S.; Onah, Joseph O.; Ladan, Joshua; Knudsen, Jenny Dahl

doi:10.1002/mbo3.816

Cited by 10 publications

(8 citation statements)

References 3 publications

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“…Several studies have reported that AmpC producer in K. pneumoniae ranged from 7.8% to 43%. [37][38][39] Similar to a previous study, resistance to carbapenem among Enterobacter spp. was high.…”

Section: Discussionsupporting

confidence: 81%

Evaluation of Resistance Mechanisms in Carbapenem-Resistant Enterobacteriaceae

Alizadeh¹,

Rezaee²,

Kafil³

et al. 2020

IDR

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Background: Carbapenem-resistant Enterobacteriaceae (CRE) is a major concern leading to morbidity and mortality in the world. CRE often is becoming a cause of therapeutic failure in both hospital and community-acquired infections. Aim: This study aimed to investigate the resistance mechanisms of CRE by phenotypic and molecular methods. Materials and Methods: Sixty CRE (50 Klebsiella pneumoniae, 6 Escherichia coli, and 4 Enterobacter spp.) were isolated from October 2018 to June 2019. Antimicrobial susceptibility testing was carried out using phenotypic methods. The carbapenem resistance mechanisms including efflux pump hyperexpression, AmpC overproduction, carbapenemase genes, and deficiency in OmpK35 and OmpK36 were determined by phenotypic and molecular methods, respectively. Results: Sixty CRE (50 Klebsiella pneumoniae, 6 Escherichia coli, and 4 Enterobacter spp.) were isolated from October 2018 to June 2019. Amikacin was found to be the most effective drug against CRE isolates. All isolates were resistant to imipenem and meropenem by the micro-broth dilution. AmpC overproduction was observed in all Enterobacter spp. and three K. pneumoniae isolates. No efflux pump activity was found. Carba NP test and Modified Hodge Test could find carbapenemase in 59 (98%) isolates and 57 (95%) isolates, respectively. The most common carbapenemase gene was bla OXA-48-like (72.8%) followed by bla NDM (50.8%), bla IMP (18.6%), bla VIM (11.8%), and bla KPC (6.7%). The ompK35 and ompK36 genes were not detected in 10 and 7 K. pneumoniae isolates, respectively. Conclusion: The amikacin is considered as a very efficient antibiotic for the treatment of CRE isolates in our region. Carbapenemase production and overproduction of AmpC are the main carbapenem resistance mechanisms in CRE isolates. Finally, Carba NP test is a rapid and reliable test for early detection of carbapenemase-producing isolates.

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Section: Discussionsupporting

confidence: 81%

Evaluation of Resistance Mechanisms in Carbapenem-Resistant Enterobacteriaceae

Alizadeh¹,

Rezaee²,

Kafil³

et al. 2020

IDR

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“…In our study, the detection rates of ESBL in E. coli- EOD and E. coli- LOD groups were 25.76 and 32.14%, respectively. These figures were significantly lower than those reported in other regions of China (45.4%) and in studies conducted overseas (53%) [ 29 , 30 ]. This is likely attributable to less frequent use of cephalosporins in the neonatal department of our hospital, more intensive monitoring of bacterial resistance, and to the regional differences.…”

Section: Discussioncontrasting

confidence: 73%

“…The widespread use of broad-spectrum antibiotics, especially the abuse of third-generation cephalosporins, is the main reason for the increase of ESBL producing strains. The mechanism of drug resistance of ESBL-positive E. coli strains is drug-induced target gene mutation; in addition, plasmid-mediated transmission among different strains leads to wide spread of drug-resistant strains [ 29 ]. In our study, the detection rates of ESBL in E. coli- EOD and E. coli- LOD groups were 25.76 and 32.14%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and antimicrobial susceptibility of invasive Escherichia coli infection in neonates from 2012 to 2019 in Xiamen, China

et al. 2021

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Background Escherichia coli (E. coli) is one of the important causative pathogens of neonatal invasive infection. The epidemiological and clinical profile of invasive E. coli infection in Chinese newborns is not well characterized. Methods Ninety-four infants with invasive E. coli infection were categorized into E. coli early onset disease (EOD) group (onset ≤72 h after birth) (n = 46) and E. coli late onset disease (LOD) group (onset > 72 h) (n = 48). We compared and analyzed the clinical characteristics and drug sensitivity profile of early-onset and late-onset E. coli invasive infection in neonates. Results The incidence of E. coli-EOD and E.coli-LOD was 0.45/1000 live births (LBs) and 0.47/1000 LBs, respectively. The incidence of gestational diabetes mellitus, perinatal fever, urinary tract infection, chorioamnionitis, and positive E. coli culture among mothers in the E. coli-EOD group were significantly higher than that in E. coli-LOD group. The incidence of premature birth, low-birth-weight, nosocomial infection, and hospitalization time were significantly higher in the E. coli-LOD group. The main disease in E. coli-EOD group was pneumonia (main clinical manifestation: dyspnea). The main disease in E. coli-LOD group was sepsis (main clinical manifestation: fever). The sensitivity rates of E. coli strains to ampicillin and piperacillin were low (25.00–28.79%); sensitivity to cephalosporins was also low except ceftazidime (lowest sensitivity rate: 57.14%). Sensitivity to compound preparations containing β-lactamase inhibitors was high, even for extended spectrum β-lactamase-positive strains (nearly 100%). Conclusion E. coli is an important cause of invasive infection of newborns in Xiamen, China. E. coli-EOD was largely attributable to perinatal factors, while E. coli-LOD was largely related to nosocomial infection. Compound preparations containing β-lactamase inhibitor or carbapenem antibiotics should be preferred for neonatal invasive infection by E. coli.

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“…Our results showed that 48.78% of analyzed Enterobacteriaceae presented the characteristic ESBL-production phenotype, being E. coli and K. pneumoniae the most common species which presented this phenotype; similar results have been reported in different countries [26,27]. Although K. pneumoniae has stood out as one of the most common producers of ESBLs, it should be noted that in recent years, ESBLproducing E. coli has gained importance worldwide [28,29].…”

Section: Esbl-producing Enterobacteriaceae Distributionsupporting

confidence: 87%

Detection of Multidrug-resistant Extended-spectrum Beta-lactamase-producing Enterobacteria from Community Infections in the city of Reynosa, Tamaulipas Mexico

Cruz-González¹,

Ramírez‐Moreno²,

Ortega-Balleza³

et al. 2020

Preprint

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Production of extended spectrum beta-lactamases (ESBL) is one of the main problems related to antimicrobial resistance worldwide, with the CTX-M, TEM and SHV types standing out as the most prevalent. These enzymes are usually related to plasmids which facilitates their horizontal genetic transmission. In the northeast region of Tamaulipas their clinical prevalence is unknown. Therefore, the aim of this work was to define the molecular epidemiology of ESBL-producing Enterobacteriaceae in clinical strains collected in Reynosa Tamaulipas, Mexico. A selection of 123 Enterobacteriaceae strains from different clinical patients were collected from August 2018 to December 2019. These strains were phenotypically identified by double disk synergy tests (DDST) and subsequently subjected to polymerase chain reaction for the detection and amplification of the blaTEM, blaSHV, blaCTX−M−1, blaCTX−M−2, blaCTX−M−9 and blaCTX−M−8/25 genes. Lastly, antimicrobial resistance profiles were determined by plate diffusion method and their capacity to transfer this sort of resistance by conjugation was assessed. Our results showed a prevalence of 48.78% (60/123) of ESBL-producing enterobacteria, with the blaTEM and blaCTX−M−1 genes most commonly detected in 76.67% (46/60) and 58.33% (35/60), respectively. Additionally, a 68.33% (41/60) of these ESBL-producing Enterobacteriaceae were multidrug-resistant, while 51.67% (31/60) were able to transfer some genes related to ESBL production, being blaCTX−M−1 the most common. This is the first study in the region that evaluates ESBL production in clinical Enterobacteriaceae strains, as well as the content of genes related to this phenotype and the ability to transfer this type of antimicrobial resistance.

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ESBL‐production in Escherichia coli and Klebsiella pneumoniae isolates from Nigeria

Cited by 10 publications

References 3 publications

<p>Evaluation of Resistance Mechanisms in Carbapenem-Resistant <em>Enterobacteriaceae</em></p>

<p>Evaluation of Resistance Mechanisms in Carbapenem-Resistant <em>Enterobacteriaceae</em></p>

Epidemiology and antimicrobial susceptibility of invasive Escherichia coli infection in neonates from 2012 to 2019 in Xiamen, China

Detection of Multidrug-resistant Extended-spectrum Beta-lactamase-producing Enterobacteria from Community Infections in the city of Reynosa, Tamaulipas Mexico

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