Erythropoietin stimulates patellar tendon healing in rats

Uslu, Mustafa; Kaya, Ertuğrul; Yaykaşlı, Kürşat Oğuz; Oktay, Murat; İnanmaz, Mustafa Erkan; Işık, Cengiz; Erdem, Havva; Erkan, Melih Engin; Kandiş, Hayati

doi:10.1016/j.knee.2015.01.011

Cited by 12 publications

(15 citation statements)

References 39 publications

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“…Manipulation of this tissue environment has therefore been an effective avenue for promotion of tendon repair (Loiselle, Kelly, & Hammert, ). Many studies indicated that the time of administration, residence time, and synergistic roles significantly complicated the use of cytokines as a treatment strategy (Guerquin et al, ; Hudgens et al, ; Uslu et al, ). Our study showed that early stage application of Lr‐PRP is a better time point compared with late stage on tendinopathy.…”

Section: Discussionmentioning

confidence: 99%

Intratendon delivery of leukocyte‐rich platelet‐rich plasma at early stage promotes tendon repair in a rabbit Achilles tendinopathy model

Jiang

et al. 2020

J Tissue Eng Regen Med

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Tendinopathy is a great obstacle in clinical practice due to its poor regenerative capacity. The influence of different stages of tendinopathy on effects of leukocyte‐rich platelet‐rich plasma (Lr‐PRP) has not been elucidated. The aim of this study is to investigate the optimal time point for delivery of Lr‐PRP on tendinopathy. A tendinopathy model was established by local collagenase injection on the rabbit Achilles tendon. Then after collagenase induction, following treatments were applied randomly on the lesion: (a) 200 μl of Lr‐PRP at 1 week (PRP‐1 group), (b) 200 μl of saline at 1 week (Saline‐1 group), (c) 200 μl of Lr‐PRP at 4 weeks (PRP‐2 group), and (d) 200 μl of saline at 4 weeks (Saline‐2 group). Six weeks after collagenase induction, outcomes were assessed by magnetic resonance imaging, cytokine quantification, gene expression, histology, and transmission electron microscopy. Our results demonstrated that PRP‐1 group had the least cross‐sectional area and lesion percent of the involved tendon, as well as the lowest signal intensity in magnetic resonance imaging among all groups. However, the PRP‐2 group showed larger cross‐sectional area than saline groups. Enzyme‐linked immunosorbent assay indicated that PRP‐1 group had a higher level of interleukin‐10 but lower level of interleukin‐6 when compared with PRP‐2 and saline groups. Meanwhile, the highest expression of collagen (Col) 1 in PRP‐1 and Col 3, matrix metalloproteinase (MMP)‐1, and MMP‐3 in PRP‐2 was found. Histologically, the PRP‐1 showed better general scores than PRP‐2, and no significant difference was found between the PRP‐2 and saline groups. For transmission electron microscopy, PRP‐1 had the largest mean collagen fibril diameter, and the PRP‐2 group showed even smaller mean collagen fibril diameter than saline groups. In conclusion, intratendon delivery of Lr‐PRP at early stage showed beneficial effect for repair of tendinopathy but not at late stage. For translation of our results to clinical circumstances, further studies are still needed.

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Section: Discussionmentioning

confidence: 99%

Intratendon delivery of leukocyte‐rich platelet‐rich plasma at early stage promotes tendon repair in a rabbit Achilles tendinopathy model

Jiang

et al. 2020

J Tissue Eng Regen Med

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“…Our literature search revealed only a single study examining the effects of systemic erythropoietin on tendon healing. In the experimental study by Uslu et al [ 12 ], the rat patellar tendons were surgically cut and then repaired, and the biomechanical and histological effects of systemic erythropoietin administration (500 U/kg body weight, similar dose with the present study) were compared with controls (no treatment or saline administration). In that study, erythropoietin administration resulted in higher ultimate breaking force when compared to controls, both at 3 and 6 weeks, suggesting better tendon healing.…”

Section: Discussionmentioning

confidence: 89%

“…Its role in wound healing has been tested in many studies using different soft tissue injury models [ 8 – 11 ]. However, studies testing its role in the healing of tendon injuries are relatively scarce [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of erythropoietin on biomechanical properties of the Achilles tendon during the healing process: an experimental study

et al. 2016

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BackgroundThe aim of this study was to examine the potential biomechanical and histological benefits of systemic erythropoietin administration during the healing of Achilles tendon injury in a rat experimental model.MethodsEighty Sprague-Dawley female rats were included in this study. Animals were randomly assigned into two groups with 40 animals in each: erythropoietin group and control group. Then each group was further divided into four subgroups corresponding to four time points with 10 animals in each. A full-thickness cut was made on the Achilles tendon of each animal and then the tendon was sutured with modified Kessler method. Erythropoietin groups received intraperitoneal erythropoietin (500 IU/kg/day) every day at same time throughout the study period, and the control groups received saline in a similar manner. Animals were sacrificed at four time points, and tensile test was performed on each tendon sample to assess maximum load for each sample. In addition, histopathological examination and scoring was done.ResultsBoth groups had improvement on tensile test (maximum load) over time. However, groups did not differ with regard to maximum load in any of the time points. Similarly, groups did not differ with regard to any of the histopathological scores over time.ConclusionsThe findings of this study do not support the benefit of systemic erythropoietin administration in Achilles tendon healing process. Further evidence from larger experimental studies is required to justify any such potential benefit.Electronic supplementary materialThe online version of this article (doi:10.1186/s13018-016-0390-1) contains supplementary material, which is available to authorized users.

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“…These molecules promote the healing of T/L through autocrine and paracrine signals (Bedi et al, 2012). Erythropoietin promotes patellar tendon healing in rats (Uslu et al, 2015). The use of carrier hydrogels to transport growth factors to repair damaged T/L is promising and provides a new possibility for bioremediation.…”

Section: Engineering Methodsmentioning

confidence: 99%

Injectable hydrogels for tendon and ligament tissue engineering

Liu

Zhang

Chen

2020

J Tissue Eng Regen Med

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The problem of tendon and ligament (T/L) regeneration in musculoskeletal diseases has long constituted a major challenge. In situ injection of formable biodegradable hydrogels, however, has been demonstrated to treat T/L injury and reduce patient suffering in a minimally invasive manner. An injectable hydrogel is more suitable than other biological materials due to the special physiological structure of T/L. Most other materials utilized to repair T/L are cell‐based, growth factor‐based materials, with few material properties. In addition, the mechanical property of the gel cannot reach the normal T/L level. This review summarizes advances in natural and synthetic polymeric injectable hydrogels for tissue engineering in T/L and presents prospects for injectable and biodegradable hydrogels for its treatment. In future T/L applications, it is necessary develop an injectable hydrogel with mechanics, tissue damage‐specific binding, and disease response. Simultaneously, the advantages of various biological materials must be combined in order to achieve personalized precision therapy.

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Erythropoietin stimulates patellar tendon healing in rats

Cited by 12 publications

References 39 publications

Intratendon delivery of leukocyte‐rich platelet‐rich plasma at early stage promotes tendon repair in a rabbit Achilles tendinopathy model

Intratendon delivery of leukocyte‐rich platelet‐rich plasma at early stage promotes tendon repair in a rabbit Achilles tendinopathy model

The effect of erythropoietin on biomechanical properties of the Achilles tendon during the healing process: an experimental study

Injectable hydrogels for tendon and ligament tissue engineering

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