Erythropoietin prevents the increase in blood–brain barrier permeability during pentylentetrazol induced seizures

Üzüm, Gülay; Diler, A. Sarper; Bahçekapılı, Nesrin; Ziylan, Y. Ziya

doi:10.1016/j.lfs.2005.10.027

Cited by 58 publications

(25 citation statements)

References 30 publications

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“…PTZ (SIGMA, USA). This dose was selected as it achieves the most successful convulsive response with the lowest mortality (8).…”

Section: Drugs and Dosesmentioning

confidence: 99%

“…Patients with epilepsy may suffer from hepatic or renal dysfunctions that interfere with their antiepileptic drug treatment (7). PTZ induced seizures are still the most widely used animal seizure models employed in the research for epilepsy and new antiepileptic drugs (8). It is reported that free radical generation plays a crucial role in neuronal cell death in the PTZ induced seizures in rats (9).…”

Section: Introductionmentioning

confidence: 99%

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EffectsofLuteolinonLiver,KidneyandBraininPentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

Birman¹,

Dar²,

Kapucu³

et al. 2012

Balkan Med J

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Objective: Flavonoids are an important group of recognized antioxidants in plants. Luteolin (LUT) is a natural flavonoid in the plant kingdom. This study was aimed to investigate the effects of the LUT in the liver, kidney and brain of pentylentetrazol (PTZ)-induced seizure and the relationship between nitric oxide synthases (iNOS, eNOS) and matrix metalloproteinases (MMP2, MMP9).Materials and Methods: LUT (10 mg/kg) was given intraperitoneally during two weeks prior to seizure induction. A single dose PTZ 80 mg/kg i.p. was administered and seizures were observed and evaluated with regard to latency, frequency and stage for one hour.Results: Seizure frequen cy after PTZ administration was significantly decreased in LUT pretreated rats (p<0.05). An increase of immunhistochemical reactions of iNOS and MMP2, but a decrease of eNOS activity, were observed in rat hippocampus and peripheral tissues during the PTZ induced seizures. LUT pretreatment reversed the iNOS and MMP2 activity to the control levels and significantly increased the eNOS activity (p<0.001).Conclusion: LUT seems to have an effective role in reducing the seizure frequency and a protective role on peripheral organ injury in animal models of seizure. The protective effect of LUT in seizures and the seizure induced peripheral tissue damage warrant further investigations.

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“…PTZ (SIGMA, USA). This dose was selected as it achieves the most successful convulsive response with the lowest mortality (8).…”

Section: Drugs and Dosesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

EffectsofLuteolinonLiver,KidneyandBraininPentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

Birman¹,

Dar²,

Kapucu³

et al. 2012

Balkan Med J

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“…Other regions of the brain show BBB breakdown only under the influence of specific convulsants. It is shown that seizures induced by pentylenetetrazole primarily affect thalamus, hypothalamus, midbrain and cerebellum Uzüm et al, 2006) (Figure 3). Also, the GABA-receptor blocker bicuculline induces loss of barrier in hippocampus whereas kainic acid induces alterations in capillaries of the neocortical brain areas (Nitsch et al, 1983).…”

Section: Blood-brain Barrier Dysfunction In Epilepsy: Experimental Anmentioning

confidence: 99%

“…Although this approach has several pitfalls as evaluation is inherently subjective, it is still used nowadays, at least as a first approach to assess BBB permeability in vivo. Such assessment can be improved by semiquantitative analysis of brain slices by fluorescence microscopy or by quantitative determination of the dye in brain homogenates (Ahishali et al, 2010;Cardosa et al, 2010;Uzüm et al, 2006;Ilbay et al, 2003;You et al 2011). Most tracers are labeled by a fluorescent dye or radioactive label that helps the quantification of the molecule (Marchi & Teng, 2010).…”

Section: Detection Of Blood-brain Barrier Dysfunction In Epilepsymentioning

confidence: 99%

The Blood-Brain Barrier and Epilepsy

İlbay¹,

Dalçįk²,

Yardımoğlu³

et al. 2012

Epilepsy - Histological, Electroencephalographic and Psychological Aspects

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“…Epo can cross the blood-brain barrier (BBB) via a receptormediated mechanism [16] . Uzum et al [17] have shown that Epo pretreatment confines BBB leakage to the cerebellum and cortical areas and lessens the intensity of tonic-clonic seizures during pentylentetrazol-induced seizures.…”

Section: Introductionmentioning

confidence: 99%

Sıçanlarda Penisilin ile Oluşturulan Epileptiform Aktivitesi Üzerine Eritropoietinin Etkileri

BULUR¹,

ANKARALI²,

DEMİR³

et al. 2015

Kafkas Univ Vet Fak Derg

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Erythropoietin (Epo), a cytokine hormone produced in the kidney, promotes the formation of red blood cells in the bone marrow. The penicillin-induced epilepsy model is a commonly used experimental model for epilepsy research. The present study was conducted to elucidate the effect of Epo on penicillin-G (500 IU/2.5 µl dose, intracortically (i.c)) -induced epileptiform activity in anesthetized adult Wistar-Albino rats (n=39). The animals were randomly divided into four groups as three treatment groups (groups 1-3) and a control group (no drug application). Rats in groups 1, 2 and 3 were intraperitoneally administered 2.000, 4.000 and 6.000 IU Epo/ kg, respectively. The effects on penicillin G induced epilepsy were compared across groups using electrocorticography. Epo at 2.000 IU/kg did not cause a significant change (P>0.05) in epileptiform spike-wave activity (number/min) and/or amplitude (µV) values, whereas the average number of spike-waves per minute and seizure severity decreased significantly in the 4.000 and 6.000 IU/kg Epo groups compared with the control (P<0.05). Consequently, the results of the present study show that administration of Epo has a dosedependent antiepileptic effect in penicillin induced model of epilepsy in rats.

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Erythropoietin prevents the increase in blood–brain barrier permeability during pentylentetrazol induced seizures

Cited by 58 publications

References 30 publications

EffectsofLuteolinonLiver,KidneyandBraininPentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

EffectsofLuteolinonLiver,KidneyandBraininPentylentetrazol-Induced Seizures: Involvement of Metalloproteinases and NOS Activities

The Blood-Brain Barrier and Epilepsy

Sıçanlarda Penisilin ile Oluşturulan Epileptiform Aktivitesi Üzerine Eritropoietinin Etkileri

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